Aglaia malabarica induced apoptosis evaluation in plant meristem, erythrocytes and human cancer cell lines
Apoptosis forms the basis of the drugs targeting cancer cells. The present study aims to explore apoptosis inducing ability of the evergreen forest plant Aglaia malabarica in three model cell systems. Allium cepa root meristem treated with the plant extract showed considerable decrease in the mitotic index and also revealed increased cytological aberration in a dose dependent manner on treatment with the leaf extract. Evans blue staining of the meristem confirmed the apoptotic death induction by the plant extract in a dose dependent way. Erythrocytes were subjected to treatment with the plant extract and remarkable apoptotic signs like membrane blebs, membrane distortions etc. were observed. HeLa cells were also characterized by the formation of echinoid bodies, membrane blebs, nuclear disintegration, fragmented nucleus etc. after treatment with A. malabarica extract. The quantitative estimation of cell viability was done using MTT assay and morphological alterations was studied by AO/EB double staining. The plant is proved to be of high potential in inducing apoptosis, in rapidly dividing cells and thereby opens a new possibility in drug preparation against uninhibited cell proliferation.
Keywords: Aglaia, apoptosis, eryptosis, chromatin aberration, MTT, HeLa
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