Intervention and Mechanism of Dexmedetomidine on A549 Cells Injured by Hypoxia/Reoxygenation
Objective: To investigate the effect and mechanism of dexmedetomidine (DEX) on hypoxia /reoxygenation (H/R) injury of A549 cells.
Methods: A549 cells were cultured and randomly divided into four groups (n=10): Normoxic group;DEX group; H/R injury group;H/R injury+DEX intervention group. Observe the morphological changes of cells; Cell viability was detected by cck-8 assay. TUNEL assay was used to detect apoptosis index (AI).Expressions of GRP78, CHOP, JNK, caspase-12, caspase-3 proteins and mRNA were detected by Western Blot and RT-PCR;Detect the activity of caspase-3.
Results: Compared with the H group, the OD value and AI value in the HD group were significantly up-regulated, apoptotic cells were significantly decreased, the expressions of CHOP, caspase-12, p-JNK and caspase-3 proteins and mRNA were significantly decreased, the GRP78 protein and mRNA increased, and the caspase-3 activity was significantly decreased, the differences were statistically significant (P<0.01).
Conclusion: Dexmedetomidine has a protective effect on A549 cells after H/R injury, which may be related to its inhibition of apoptosis induced by excessive endoplasmic reticulm stress.
Keywords: Dexmedetomidine; Hypoxia/Reoxygenationinjury;Apoptosis;Endoplasmicreticulm stress.
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