Intervention and Mechanism of Dexmedetomidine on A549 Cells Injured by Hypoxia/Reoxygenation

  • Hui Gao Ischemia/Reperfusion Injury Research Institute of Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China
  • Ziyin Luo Ischemia/Reperfusion Injury Research Institute of Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China
  • Zhuolin Zhou Ischemia/Reperfusion Injury Research Institute of Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China
  • Yiming Wu Division of Cardiovascular Medicine University of Iowa Carver College of Medicine Iowa City, American 50309
  • Wantie Wang Ischemia/Reperfusion Injury Research Institute of Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China

Abstract

Objective: To investigate the effect and mechanism of dexmedetomidine (DEX) on hypoxia /reoxygenation (H/R) injury of A549 cells.


Methods: A549 cells were cultured and randomly divided into four groups (n=10): Normoxic group;DEX group; H/R injury group;H/R injury+DEX intervention group. Observe the morphological changes of cells; Cell viability was detected by cck-8 assay. TUNEL assay was used to detect apoptosis index (AI).Expressions of GRP78, CHOP, JNK, caspase-12, caspase-3 proteins and mRNA were detected by Western Blot and RT-PCR;Detect the activity of caspase-3.


Results: Compared with the H group, the OD value and AI value in the HD group were significantly up-regulated, apoptotic cells were significantly decreased, the expressions of CHOP, caspase-12, p-JNK and caspase-3 proteins and mRNA were significantly decreased, the GRP78 protein and mRNA increased, and the caspase-3 activity was significantly decreased, the differences were statistically significant (P<0.01).


Conclusion: Dexmedetomidine has a protective effect on A549 cells after H/R injury, which may be related to its inhibition of apoptosis induced by excessive endoplasmic reticulm stress.


Keywords: Dexmedetomidine; Hypoxia/Reoxygenationinjury;Apoptosis;Endoplasmicreticulm stress.

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Author Biographies

Hui Gao, Ischemia/Reperfusion Injury Research Institute of Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China

Ischemia/Reperfusion Injury Research Institute of Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China

Ziyin Luo, Ischemia/Reperfusion Injury Research Institute of Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China

Ischemia/Reperfusion Injury Research Institute of Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China

Zhuolin Zhou, Ischemia/Reperfusion Injury Research Institute of Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China

Ischemia/Reperfusion Injury Research Institute of Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China

Yiming Wu, Division of Cardiovascular Medicine University of Iowa Carver College of Medicine Iowa City, American 50309

Division of Cardiovascular Medicine University of Iowa Carver College of Medicine Iowa City, American 50309

Wantie Wang, Ischemia/Reperfusion Injury Research Institute of Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China

Ischemia/Reperfusion Injury Research Institute of Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China

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How to Cite
Gao, H., Luo, Z., Zhou, Z., Wu, Y., & Wang, W. (2019). Intervention and Mechanism of Dexmedetomidine on A549 Cells Injured by Hypoxia/Reoxygenation. Journal of Drug Delivery and Therapeutics, 9(3), 99-106. https://doi.org/10.22270/jddt.v9i3.2607