Determination of Minimum Inhibitory Concentration (MIC) and percentage Bacterial Growth Inhibition of essential oils against Gram Positive Bacterial pathogens

  • S. Nagalakshmi Department of Biochemistry, Sacred Heart College (Autonomous), Tirupattur, Vellore district, Tamil Nadu, India.
  • P. Saranraj Department of Microbiology, Sacred Heart College (Autonomous), Tirupattur, Vellore district, Tamil Nadu, India.
  • P. Sivasakthivelan Department of Agricultural Microbiology, Faculty of Agriculture, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu, India.

Abstract

Essential oils and volatile constituents extracted from Aromatic plants are frequently used in folk medicine for prevention and treatment of different human diseases. The urge to develop alternative treatment strategies follows three different directions. In the present study, an attempt has been planned to determine the Minimum Inhibitory Concentration (MIC) and Percentage Growth Inhibition of Essential oils against two Gram positive bacterial pathogens, Staphylococcus aureus and Bacillus subtilis. The Essential oils selected for the present study was collected from Sidha Medicine Shop, Tirupattur, Vellore district, Tamil Nadu, India. The Broth dilution method was used for the determination of Minimum Inhibitory Concentration (MIC) of Essential oils. The Minimum Inhibitory Concentration (MIC) studies were conducted by using various concentrations of Essential oils viz., 25 µl/ml, 50 µl/ml, 75 µl/ml and 100 µ/ml. The Essential oils exhibits inhibitory activity against Gram positive bacteria in all the concentrations. Among the seven Essential oils tested, Mahualongif oil has showed maximum percentage bacterial growth inhibition when compared to other Essential oils. The inhibitory activity of Mahualongif oil was observed more in Staphylococcus aureus when compared to Bacillus subtilis. For Staphylococcus aureus, lowest inhibitory percentage was noticed in Pungam oil and for Bacillus subtilis lowest inhibitory percentage was noticed in Coconut oil.


Keywords: Essential oils, Minimum inhibitory concentration (MIC), Percentage bacterial growth inhibition, Bacillus subtilis and Staphylococcus aureus.

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Author Biographies

S. Nagalakshmi, Department of Biochemistry, Sacred Heart College (Autonomous), Tirupattur, Vellore district, Tamil Nadu, India.

Department of Biochemistry, Sacred Heart College (Autonomous), Tirupattur, Vellore district, Tamil Nadu, India.

P. Saranraj, Department of Microbiology, Sacred Heart College (Autonomous), Tirupattur, Vellore district, Tamil Nadu, India.

Department of Microbiology, Sacred Heart College (Autonomous), Tirupattur, Vellore district, Tamil Nadu, India.

P. Sivasakthivelan, Department of Agricultural Microbiology, Faculty of Agriculture, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu, India.

Department of Agricultural Microbiology, Faculty of Agriculture, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu, India.

References

1) Barnes, J., Anderson, J and Phillipson, J. D. Herbal Medicines, 3rd Pharmaceutical Press, London, 2007; 63(1):23 - 26.
2) Sekhri, K. Antimicrobial Resistance Understanding Solutions and Future Development. International Journal of Pharmaceutical and Biological Science, 2013; 4(2):338-343.
3) Rota, M. C., Herrera, A., Martinez, R. M., Sotomayor, J. A and Jordan, M. J. Antimicrobial activity and chemical composition of Thymus vulgaris, Thymus zygis and Thymus hyemalis. Food Control, 2008; 19:681 - 687.
4) Bakkali, F., Averbeck, S., Averbeck, D and Idaomar, M. Biological Effects of Essential oils – A Review. Food Chemistry and Toxicology, 2008; 46:446 - 475.
5) Vukovic, N., Milosevic, T., Sukdolak, S and Solujic, S. Antimicrobial activities of Essential oil and Methanol extract of Teucrium montanum. Complementary and Alternative Medicine, 2007; 4:17 - 20.
6) Miguel, M. G., Duarte, J., Figueiredo, A. C., Barroso, J. G and Pedro, L. G. Thymus carnosus Boiss. - Effect of harvesting period, collection site and type of plant material on essential oil composition. Journal of Essential Oil Research, 2005; 17(4):422 -426.
7) Angioni, A., Barra, A., Coroneo, V., Dessi, S. and Cabras, P., Chemical composition, seasonal variability, and antifungal activity of Lavandula stoechas L. ssp. Stoechas essential oils from stem/leaves and flowers. Journal of Agriculture Food Chemistry, 2006; 54(12):4364–4370.
8) Freires, I. A., Denny, C., Benso, B., Alencar, S. M and Rosalen, P. Antibacterial activity of essential oils and their isolated constituents against carcinogenic bacteria: A Systematic Review. Molecules, 2015; 20:7329 - 7358.
9) Yap, P. S. X., Yiap, B. C., Ping, H. C. and Lim, S. H. E. Essential oils - A new horizon in combating bacterial antibiotic resistance. The Open Microbiology Journal, 2014; 8:6 - 14.
10) Ericsson, H. M. and J.C. Sherris. 1971. Antibiotic sensitivity testing. Report of an International Collaborative Study. Acta. path. Microbiol. Scand., Sec. B, Suppl. No.217.
11) Lezcano, N., Nunez, M., Espim, I., Dupre, A., Pinna, P., Molicotti, G., Fadda and Zanetti, S. (2018). Department of Science Biomedich Degli University Study, Sassari Italy.
12) Singh, N. Impact of Current Transplantation Practices on the Changing Epidemiology of Infections in Transplant Recipients. Lancet Infectious Diseases, 2019; 3:156–161.
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How to Cite
Nagalakshmi, S., Saranraj, P., & Sivasakthivelan, P. (2019). Determination of Minimum Inhibitory Concentration (MIC) and percentage Bacterial Growth Inhibition of essential oils against Gram Positive Bacterial pathogens. Journal of Drug Delivery and Therapeutics, 9(3), 33-35. https://doi.org/10.22270/jddt.v9i3.2596