Investigation of phytoconstituents of Cardiospermum halicacabum and its efficacy as a potential anti-cancer drug candidate
On the basis of the traditional knowledge of treating solid tumor using Cardiospermum halicacabum gained attention to carry out the present work. The preliminary screening for determination of cytotoxicity of successive extract of C halicacabum (hexane, chloroform, ethyl acetate and ethanol) was performed through brine shrimp lethality assay and yeast growth rate cytotoxicity assay. Among the extracts, chloroform extract of C halicacabum exhibited highest cytotoxicity which was further investigated for anti-proliferative and cytotoxicity property in A-549 lung cancer cell line. Correspondingly, dose dependent effects on reduction of cell proliferation with prominent morphological abnormalities of A-549 cells were observed under treatment with chloroform extract. Hemocompatibility assessment of chloroform extract, by hemolysis assay, revealed its compatibility towards RBC which in turn may prevent heamolytic anemia (myelosuppression) the most adverse effects of cancer chemotherapy. Phytoconstituents of chloroform extract responsible for the cytotoxicity, anti-proliferative and hemocompatibiltiy was assessed by several chromatographic methods such as TLC, Column chromatography and HPLC which revealed the presence of flavonoids in chloroform extract of C halicacabum. Apparently the isolated flavonoids, in consistence with chloroform extract, exhibited similar effect on inhibition of proliferation of tumor cell line A-549. Therefore it could be evidenced from the current study that the C halicacabum, with its predisposed flavonoids, possesses anti-cancer property with least adverse effect on heamolysis. However detailed investigation on regulation of tumor cell proliferation and hemocompatability is required to bring C halicacabum as a potential candidate for cancer therapy.
Keywords: C halicacabum, Anti-cancer, Toxicity, Brine Shrimp Lethality Assay, Hemolysis.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).