Role of Roof plate-specific SPONDIN3 Mutation in the Determination of Obesity Phenotypes/Fat Distribution and Susceptibility to Cardiovascular disease in Sudanese Patients in Khartoum State

  • Ashraf M.A. Alkinain Shendi University College of Medical Laboratory, Clinical Chemistry, Shendi, River Nile State, Sudan
  • Kamal Eldin Ahmed Abdelsalam Faculty of Medical Laboratory Sciences, Seconded to Shaqra University, Saudi Arabia
  • Mutaz Ibrahim Hassan Faculty of Medical Laboratory Sciences, Seconded to Shaqra University, Saudi Arabia
DOI https://doi.org/10.22270/jddt.v9i2.2535

Abstract

Background: Obesity is a major risk factor for the development of cardiovascular disease. A growing database of clinical evidence implicates intra-abdominal adiposity as a powerful driving force for elevated cardiometabolic risk (1). Addressing intra-abdominal adiposity should play a central role in future strategies aimed at improving cardiovascular outcomes in patients with abdominal obesity and its associated cardiometabolic risk in Sudan.


Objectives: It is to find the mutation in R-SPONDIN3gene and its association to both of fat deposition around the abdomen and susceptibility to cardiovascular disease in Sudanese patients in Khartoum State.


Material and methods: Conventional PCR was done to detect R- SPONDIN3 in 300 participants (males and females) classified into three groups. The first group will include one hundred participants with abdominal obesity, the second group will include one hundred participants already diagnosed with CVD entangled with obesity (positive control group), while the third group will include one hundred healthy lean volunteers (negative control group). Data was analyzed using SPSS Version 22 software. P value < 0.05 was considered as statistically significant.


Results: In this study, the results of Conventional PCR were significantly different in (P <0.001) in Heart group subjects as compared to healthy controls and obese group. Comparison between the different studied groups according to gene expression showed significant differences (P <0.001) mean value of gene expression in healthy group subjects was 1.0 ± 0.0, Obesity group was 2.44 ± 0.50 and heart group subjects was 4.54 ± 0.87 respectively .


Conclusion: clinically, detect R- SPONDIN3 mutation in patients with diagnosed with CVD entangled with obesity and amount of the gene expressed cleared different between obese and CVD subjects entangled with obesity.


Keywords: R-SPONDIN3 gene, abdominal Obesity, CVD.

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Author Biographies

Ashraf M.A. Alkinain, Shendi University College of Medical Laboratory, Clinical Chemistry, Shendi, River Nile State, Sudan

Shendi University College of Medical Laboratory, Clinical Chemistry, Shendi, River Nile State, Sudan

Kamal Eldin Ahmed Abdelsalam, Faculty of Medical Laboratory Sciences, Seconded to Shaqra University, Saudi Arabia

Faculty of Medical Laboratory Sciences, Seconded to Shaqra University, Saudi Arabia

Mutaz Ibrahim Hassan, Faculty of Medical Laboratory Sciences, Seconded to Shaqra University, Saudi Arabia

Faculty of Medical Laboratory Sciences, Seconded to Shaqra University, Saudi Arabia

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1.
Alkinain A M, Abdelsalam KEA, Hassan MI. Role of Roof plate-specific SPONDIN3 Mutation in the Determination of Obesity Phenotypes/Fat Distribution and Susceptibility to Cardiovascular disease in Sudanese Patients in Khartoum State. JDDT [Internet]. 15Mar.2019 [cited 2Mar.2021];9(2):118-24. Available from: http://jddtonline.info/index.php/jddt/article/view/2535
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Vol 9 No 2 (2019): Volume 9, Issue 2 March-April 2019
Section
Research
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