Evaluation of antifungal activity of Magnesium oxide (MgO) and Iron oxide (FeO) nanoparticles on rot causing fungi
The antifungal activity of Magnesium oxide (MgO) and iron oxide (FeO) nanoparticles prepared by bio safe method was evaluated for Penicillium expansum, Aspergillus niger, Alternaria alternata, Mucor plumbeus, Penicillium chrysogenum, Trichothecium roseum and Rhizoctonia solani. It was observed from the study that all the concentrations of nanoparticles brought about significant inhibition in the spore germination and mycelial growth of all the rot causing fungi. However, the highest inhibition in the germination of all the test fungi was observed at higher concentrations followed by lower concentrations of nanoparticles. It was observed from the present study that MgO and FeO nanoparticles showed signiﬁcant antimycotic activity against all the tested fungal pathogens. However, highest reduction in spore germination was observed against Mucor plumbeus whereas least reduction of spore germination was observed against Aspergillus niger at different concentration of nanoparticles of MgO respectively. Likewise, the maximum inhibition in the fungal growth was observed against Alternaria alternate and least inhibition in zone of fungal growth due to MgO nanoparticle was found against Mucor plumbeus respectively. Similarly, highest inhibition in spore germination was found against Penicillium expansum and least inhibition in spore germination was found against Aspergillus niger at different concentrations of nano FeO. Likewise, the maximum inhibition in the fungal growth was found against Rhizoctonia solani and Trichothecium roseum and least inhibition in zone of fungal growth was found against Penicillium expansum and Mucor plumbeus at 0.1mg/ml, 0.25mg/ml and 0.5 mg/ml concentrations of FeO nanoparticles respectively. Activity index was recorded highest against P. chrysogenum (0.97) in case of MgO and against T. roseum (0.97) in case of FeO nanoparticles respectively.
Keywords: Spore germination, mycelial growth, rot causing fungi, tomato, brinjal, MgO and FeO nanoparticle
2. Mansoori GA. Principles of Nanotechnology – Molecular-Based Study of Condensed Matter in Small Systems, World Scientific Pub. Co., Hackensack, NJ. 2005.
3. Mansoori GA. George TF. Zhang G. Assoufid L. Molecular Building Blocks for Nanotechnology, Springer, New York, 2007.
4. Park HJ. Kim SH. Kim HJ. Choi SH. A new composition of nanosized silica silver for control of various plant diseases. Plant Pathol. J. 2006; 22:25-34.
5. Raghupatri RK. Koodali RT. Manna AC. Size dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles. Langmuir. 2011; 27:4020-4028.
6. Kiraly Z. Klement Z. Solymosy F. Voros J. Methods in Plant Pathology with special reference to breeding for resistance to breeding for resistance, Elsevier publishing company, New York.1974. p. 212
7. Perez C. Pauli M. Bazerque P. An antibiotic assay by the well agar method. Acta Biolog, Med. Experiment. 1990; 15:113-115.
8. Norrel SA. Messley KE. Microbilogy Laboratory Manual Principles and Applications, Prentice Hall, Upper Saddle River. New Jersey. 1997. 85-90.
9. Singariya P. Kumar P. Mourya KK. Antimicrobial activity of friut coat (calyx) of Withania somnifera against some multi drug resistant microbes. Int. J. Biol. Pharmaceut. Res. 2012; 2:252-258.
10. Morones JK. Elechiguerra JL. Camacho A. Holt K. Kouri JB. Ramirez JT. Yacaman J. The bacterial effect of silver nanoparticles. Nanotechnology. 2005; 16(10):2346-2353.
11. Kim SW. Kim KS. Lamsal K. Kim YJ. Kim SB. Jung M. Sim SJ. Kim HS. Chang SJ. Kim JK. Lee YS. An in vitro study of the antifungal effect of silver nanoparticles on oak wilt pathogen, Raffaelea sp. J. Microbiol. Biotechnol. 2009; 19:760-764.
12. Jo YK. Kim BH. Jung G. Antifungal activity of silver ions and nanoparticles on Phytopathogenic fungi. Plant Disease. 2009; 03:1037-1043.
13. Parizi MA. Moradpour Y. Roostaei A. Khani M. Negahdari M. Rahimi G. Evaluation of the antifungal effect of magnesium oxide nanoparticles on Fusarium oxysporum F. Sp. lycopersici, pathogenic agent of tomato. European Journal of Experimental Biology, 2014; 4(3):151-156.
14. Bhainsa KC. D’Souza SF. Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigates. Colloids Surf B Biointerfaces. 2006; 47(2):160-164.
15. Vahabi K. Mansoori GA. Karimi S. Biosynthesis of silver nanoparticles by fungus Trichoderma reesei. Inscience J. 2011; 1:65-79.
16. Wani AH. Shah MA. A unique and profound effect of iron oxide and ZnO nanoparticles on some pathogenic fungi. J. Appl. Pharmaceut. Sci. 2012; 2:40-44.
17. Wani AH. Amin M. Shahnaz M. Shah MA. Antimycotic activities of MgO, FeO and ZnO on some pathogenic fungi. Int. J. Manuf. Mater. Mech. Eng. 2012; 2(4):59-70.
18. Pulit J. Banach M. Szczyglowska R. Bryk M. Nanosilver against fungi. Silver nanoparticles as an effective biocidal factor. Acta Biochim. Pol. 2013; 60(4):795-798.
19. Nehra P. Chauhan RP. Garg N. Verma K. Antibacterial and antifungal activity of chitosen coated iron oxide nanoparticles, Br. J. Biomed. Sci. 2017. https://doi.org/10.1080/09674845.2017.1347362.
20. Parveen S. Wani AH. Shah MA. Devi HS. Bhat MY. Koka JA. Preparation, Characterisation and antifungal activity of iron oxide nanoparticles. Microbial Pathogenesis. 2018; 115:287-292.
21. Abdeen S. Issac RR. Geo S. Somalekshmi S. Rose A. Praseetha PK. Evaluation of antimicrobial activity of biosynthesized iron and silver nanoparticles using the fungi Fusarium oxysporum and Actinomyces sp. on human pathogens. Nano Biomed. Eng. 2013; 5(1):39-45.
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