Pharmacological Activity of a Polyherbal Formulation by Haemoglobin Glycosylation Assay
Present study involves the development of a polyherbal formulation by using four different herbs i.e. Chirata (Swertia chiratia), Haldi (Curcuma longa), Neem (Azadirachta indica), Gudmar (Gymnema sylvestre), Ashwagandha (Withania somnifera), Gokharu (Pedalium murex), Methi (Trigonella foenum-graecum), Jammun (Syzygium cumini), relating to antidiabetic activity. Freshly collected and authenticated herbs were characterized by studying its morphological and pharmacognostic character. Antidiabetic and antioxidant activity of the formulation was determined by in vitro haemoglobin glycosylation assay and H2O2 radical scavenging method respectively. In the above study it was found that ethanolic extract of polyherbal formulation possess promising antidiabetic and antioxidant activity which can be consider for further biological investigation.
Keywords: polyherbal formulation, Swertia chiratia, Curcuma longa, Azadirachta indica, Gymnema sylvestre, Withania somnifera, Pedalium murex, Trigonella foenum-graecum, Syzygium cumini
2. American Diabetes Association., Classification and diagnosis of diabetes, Diabetes Care 38, Suppl, 2015; S8–S16: doi: 10.2337/dc15-S005.
3. Shelke DP, Shendarkar GR, Daswad AK, Pohare JG, Wangawar SN, Design and development of polyherbal formulation with antioxidant potential, Journal of Medicinal Plants Studies, 2017; 5(2):207-213.
4. Rahim NFA, Muhammad, Abdullah N, Talip AB, Dusuks NJS, Synergistic Effect of Polyherbal Formulations on DPPH Radical Scavenging Activity, Journal of Science and Technology, 2018; 10:116-121.
5. Sharma PR, Laware SL, IN-VITRO ANTIDIABETIC EFFECT OF POLYHERBAL FORMULATION, Indo American Journal of Pharmaceutical Research 2015; 5(6):2402-2407.
6. Sindhu SN, Vaibhavi K, Anshu M, Evaluation of In Vitro Anti diabetic Activity of Selected Plant Extracts, International Journal of Pharmaceutical Science Invention, 2013; 2(4):12-19.
7. Jyoti M, Agarwal K, Sharma V, Saini P, Preparation and Standardization of A Polyherbal Formulation, J Adv Scient Res, 2012; 3(2):84-85.
8. Madhuri VR, Begum SP, Begum HS, Kumar PA, Mahesh S, Karimulla S, Nagarajan G, Evaluation of in vitro anti-diabetic activity on ethanolic extract of aerial parts of Murrayakoenigii, Indian Journal of Research in Pharmacy and Biotechnology, 2016; 4(3):2320 – 3471.
9. Kavitha P, Sowmia C, SCREENING OF PHYTOCHEMICAL AND IN-VITRO ANTIOXIDANT PROPERTY OF A POLYHERBAL FORMULATION, IJPSR 2016; 7(11):4608-4614.
10. Shreedhara CS, Ram HNA, Zanwar SB, Gajera FP, IN VITRO ATIOXIDAT POTETIAL OF ETHAOLIC EXTRACT OF MOMORDICA DIOICA ROXB (CUCURBITACEAE), Pharmacologyonline, 2011; 3:622-633.
11. Padmanabhan P, Jangle S, In-vitro Antioxidant Potential of a Herbal Preparation Containing Four Selected Medicinal Plants, 2012,1,(2) 52-63.
12. Nair SS, Kavrekar V, Mishra A, Evaluation of In Vitro Anti diabetic Activity of Selected Plant Extracts, International Journal of Pharmaceutical Science Invention, 2013; 2(4):12-19.
13. Ajithadas A, Synthesis and Characterization of Silver Nanoparticles of Insulin Plant (costus pictus D. Don) Leaves, Asian Journal of Biomedical and Pharmaceutical Sciences, 2014; 4(34):1-6.
14. Babu S, Prince, GREEN SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES USING Pterocarpus marsupium AND ASSESSMENT OF ITS IN-VITRO ANTI DIABETIC ACTIVITY, J Pharm Pharmacol, 2004; 56:1435-1442.
15. Rohit K, Anania A, Diksha, Reena G, Sanchit M, Saurabh S, Meenu M, In Vitro Antioxidant and Antimicrobial Activity of Polyherbal Formulation, International Journal of Green Pharmacy, 2018; 80-83.
16. Savant D, Panchal P, Evaluation of Antidiabetic and Antioxidant activities of Madhumardanchuran, IJPRD, 2013; 5(3) .
17. James, Auta SA, Goje R, IN VITRO STUDY ON INHIBITION OF GLYCOSYLATION OF METHANOLIC LEAF EXTRACT OF Hibiscus cannabinus, Science World Journal, 2011; 6 (3):7-9.
18. Efrilia, Iskandar T, Khairina, Suhartono E, Antidiabetic and Antioxidant Activity of Jackfruit (Artocarpus Heterophyllus) Extract, Journal of Medical and Bioengineering, 2015; 4(4):318-323.
19. Mulla WA, Salunkhe VR, Kuchekari SB, Qureshi MN, Free Radical Scavenging Activity of Leaves of Alocasia indica, J. Pharm. Sci., 2009; 71 (3):303-307.
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