Estimation of total Phenol and flavonoid contents of Citrus limon L Burmf leaves from North Eastern region of India.
Citrus plants belonging to the family Rutaceae, are traditionally used by the local people India to treat scurvy, rheumatism, stomachic, diarrhea, liver disorders and used as an antioxidant. They are valuable source of phenolic and other biologically active compounds. Phenolic compounds have been reported to have multiple biological effects. In this paper quantitative determination of phenols and flavonoids of Citrus limon L Burmf leaves extracts was carried out using spectrophotometric methods. The plant material was collected from Assam, north eastern region of India. Total phenolic content was determined by Folin-Ciocalteu method and aluminium chloride method was used for flavonoid determination. Acetone and methanol extracts of leaves showed quite high amount of phenol as well as flavinoid contents compared to ethanol and ethyl acetate extracts. However, all the extracts exhibited significant results.
Keywords: Citrus limon, Phenol, Flavonoid, Catechol and Quercetin.
2. Citrus fruit biology, tehnology and evaluation. Ladaniya: Academic Press; 2008.
3. Mohammadian MA, Mobram ZI, Sajedi RH, Bioactive compounds and antioxidant capacities in the flavedo tissue of two citrus cultivars under low temperature, Braz J Plant Physiol, 2011; 23(3):203-208.
4. Mukhim C, Nath A, Deka B, Swer TL, The Bioscan, 2015; 10(2):535-537.
5. Varies PS, Indian medicinal plants. Kottakkal: Arya Vaidya Sala; 2006; 2:97-100.
6. Munne S, Parwate D, Ingle V, NagpurkarV, Evaluation of the anthelmintic activity of citrus limon juice sacs, Int J Pharma Professional’s Research, 2011; 2(2).
7. Barua P, Yield, fruit quality and water productivity of drip fertigated Assam Lemon (Citrus limon). Internat J Agric Engg., 2013; 6(2):339-344.
8. Chaliha BP, Barua AD, Siddappa GS, Assam limon as a source of pactin, Indian Food Packer, 1963, XVII(3).
9. Sarmah N, Kumari S, Comparative study of antibacterial activity of ripen and unripen Indigenous Citrus union of Assam, India, Int J Advancements in Research & Technology, 2013; 2(9):25-31.
10. Deb Roy S, Bania R, Chakraborty J, Goswami R, Laila R, Ahmed SA, Pharmacognostic, phytochemical, physicochemical property and antimicrobial activity studies of lemon peel oil, Nat Prod Plant Resource, 2012; 2(3):431-435.
11. Muthiah PL, Umamaheswari M, Kumar AK, In vitro antioxidant activities of leaves, fruits and peel extracts of citrus, International Journal of Phytopharmacy Research Article, 2012; 2(1):13-20.
12. E. Khatiwora, V. Adsul, V. Arbale, S. Gaikwad, Spectroscopic determination of total phenol and flavonoid contents of citrus limon peel from northeastern India, JDDT, 2017; 7 (1):21-24.
13. Malik EP, Singh MB, Plant Enzymology and Hittoenzymology. 1st Ed. New Delhi: Kalyani Publishers; 1980, 286.
14. Mervat MM, H El-Far, AA Taie, Antioxidant activities, total anthrocynins, phenolics and flavonoids contents of some sweet potato genotypes under stress of different concentrations of sucrose and sorbitol, Australian J Basic Applied Sc, 2009; 3:3609-3616.
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