Evaluation of Ocimum tenuiflorum and Syzygium aromaticum phenolic ethereal oils for In-vitro anti-inflammatory and anti-bacterial activities
Aim: The present study is aimed to evaluate in-vitro anti-inflammatory and anti-bacterial activity of phenolic ethereal oils like Tulsi (Ocimum tenuiflorum) and Clove (Syzygium aromaticum).
Materials and methods: A total of 500 g of fresh leaves and dried flower buds of Tulsi and Clove were subjected to hydro-distillation method for 6 h using Clevenger’s apparatus. The isolated ethereal oils were used for testing the in-vitro anti-inflammatory activity by using albumin denaturation assay, proteinase inhibitory activity at a concentration of 20, 40, 60, 80 and 100 µl/ml and anti-bacterial activity against two gram positive microorganisms (Bacillus subtilis and Staphylococcus aureus) and two gram negative microorganisms (Salmonella typhi and Escherichia coli) at concentrations 50 µl/ml, 100 µl/ml and 200 µl/ml by adopting cup plate method.
Results: The isolated ethereal oils exhibited significant in-vitro anti-inflammatory effect and also inhibited the growth of both Gram positive and Gram negative microorganisms at 50 µl/ml, 100 µl/ml and 200 µl/ml concentrations.
Conclusion: The findings of this study showed that the effectiveness of Phenolic ethereal oils isolated from Clove and Tulsi. Clove (Syzygium aromaticum) showed enhanced anti-inflammatory and anti-bacterial activity compared to Tulsi (Ocimum tenuiflorum). The present study provides evidence that Ocimum tenuiflorum and Syzygium aromaticum; Phenolic ethereal oils contain medicinally important bioactive components justifying its traditional use.
Keywords: Phenolic ethereal oils, anti-inflammatory activity, anti-bacterial activity, Ociumm tenuiflorum, Syzygium aromaticum.
2. Mohamed E, Ibrahim M, Hend E. Introduction of Ocimum tenuiflorum plant to the Egyptan Cultivation. International Journal of PharmaTeach Research 2016; 9:18-24.
3. Joshi RK, Hoti SL. Chemical composition of the essential oil of Ocimum tenuiflorum L. from North West Karnataka, India. Plant Science Today 2014; 1:99-102.
4. Diego F, Cortes R, Claudia R, Fernandes S. Clove: a precious spice. Asian pac J Trop Biomed 2014; 4:90-6.
5. Dagli N, Dagli R, Mahmoud RS, Baroudi K. Essential oils their therapeutic properties, and implication in dentistry: A review. J Int Soc Prev Community Dent 2015; 5:335-40.
6. Muchalal M, Crouzet J. Volatile components of clove essential oil (Eugenia caryophyllus spreng) Neutral fraction. Agric Biol Chem 1985; 49:1583-9.
7. Sangita C, Priyanka C, Protapaditya D, Sanjib B. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pacific Journal of Tropical Biomedicine 2012; 178-80.
8. Oyedepo O, Femurewa AJ. Anti‐protease and membrane stabilizing activities of extracts of Fagrazanthoxiloides, Olaxsubscorpioides and Tetrapleura tetraptera. Int J of Pharmacong 1995; 33:65‐9.
9. Nyiligira E, Viljoen VM, Vuuren SF. Essential oil composition and in vitro antimicrobial and anti-inflammatory activity of South African Vitex species. South African Journal of Botany 2004; 70:611–7.
10. Ashraf A, Mostafa A, Khalid S, Turki M. Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi Journal of Biological Sciences 2018; 25:361-6.
11. Narender Boggula. Phytochemical evaluation and in vitro anti bacterial activity of dried seeds of Abrus precatorius. International journal of pharmaceutical sciences review and research 2017; 44(1):101-7.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).