Evaluation of Ocimum tenuiflorum and Syzygium aromaticum phenolic ethereal oils for In-vitro anti-inflammatory and anti-bacterial activities

  • N.K. Manaswini Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.
  • S Nazneen Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.
  • Garige Baba Shankar Rao Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.
  • Boggula Narender Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.
  • Bakshi Vasudha Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.
  • Manda Ram Mohan Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.

Abstract

Aim: The present study is aimed to evaluate in-vitro anti-inflammatory and anti-bacterial activity of phenolic ethereal oils like Tulsi (Ocimum tenuiflorum) and Clove (Syzygium aromaticum).


Materials and methods: A total of 500 g of fresh leaves and dried flower buds of Tulsi and Clove were subjected to hydro-distillation method for 6 h using Clevenger’s apparatus. The isolated ethereal oils were used for testing the in-vitro anti-inflammatory activity by using albumin denaturation assay, proteinase inhibitory activity at a concentration of 20, 40, 60, 80 and 100 µl/ml and anti-bacterial activity against two gram positive microorganisms (Bacillus subtilis and Staphylococcus aureus) and two gram negative microorganisms (Salmonella typhi and Escherichia coli) at concentrations 50 µl/ml, 100 µl/ml and 200 µl/ml by adopting cup plate method.


Results:  The isolated ethereal oils exhibited significant in-vitro anti-inflammatory effect and also inhibited the growth of both Gram positive and Gram negative microorganisms at 50 µl/ml, 100 µl/ml and 200 µl/ml concentrations.


Conclusion: The findings of this study showed that the effectiveness of Phenolic ethereal oils isolated from Clove and Tulsi. Clove (Syzygium aromaticum) showed enhanced anti-inflammatory and anti-bacterial activity compared to Tulsi (Ocimum tenuiflorum). The present study provides evidence that Ocimum tenuiflorum and Syzygium aromaticum; Phenolic ethereal oils contain medicinally important bioactive components justifying its traditional use.


Keywords: Phenolic ethereal oils, anti-inflammatory activity, anti-bacterial activity, Ociumm tenuiflorum, Syzygium aromaticum.

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Author Biographies

N.K. Manaswini, Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.

Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of        Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.

S Nazneen, Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.

Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of        Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.

Garige Baba Shankar Rao, Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.

Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of        Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.

Boggula Narender, Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.

Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of        Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.

Bakshi Vasudha, Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.

Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of        Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.

Manda Ram Mohan, Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.

Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag Group of Institutions, Venkatapur, Ghatkesar, Medchal, Telangana, India.

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How to Cite
Manaswini, N., Nazneen, S., Shankar Rao, G. B., Narender, B., Vasudha, B., & Mohan, M. R. (2019). Evaluation of Ocimum tenuiflorum and Syzygium aromaticum phenolic ethereal oils for In-vitro anti-inflammatory and anti-bacterial activities. Journal of Drug Delivery and Therapeutics, 9(2), 93-96. https://doi.org/10.22270/jddt.v9i2.2383