Assessment of anti-oxidant activity of Ficus benghalensis leaves: an In-vitro design
Background: Medicinal plants represent a rich source of natural anti-oxidants. The traditional medicine involves the use of different plant extracts of bioactive constituents. Ficus benghalensis is a large evergreen tree found throughout India. It is commonly called Banyan tree. It is used in traditional system of medicine like Ayurveda and homoeopathy. Ficus is a huge tropical deciduous or evergreen tree with more than 800 species.
Objective: Anti-oxidant effect of ethnomedicinal important plant species Ficus benghalensis (Family-Moraceae) was screened. The aim of this study was to assess the in-vitro potential of methanolic extract of Ficus benghalensis leaves as a natural anti-oxidant.
Method: Assessment of in vitro anti-oxidant activity of methanolic leaves extract of Ficus benghalensis was determined by DPPH free radical scavenging assay.
Results: The methanolic extract of Ficus benghalensis leaves demonstrates a remarkable amount of phenolic content (54 μg/ml of GAE/100gm of extract) by using Folin-ciocalteu reagent. In-vitro anti-oxidant activity of methanolic leaves extract of Ficus benghalensis was determined by DPPH free radical scavenging assay, which showed significant anti-oxidant activity compared to standard anti-oxidant (ascorbic acid). A direct correlation between total phenol and anti-oxidant activity indicates polyphenols are the main anti-oxidants.
Conclusion: These findings suggest the excellent medicinal bio-activity of Ficus benghalensis and explain the popularity of this plant in the folk medicine as a remedy for different disorders, thus supporting its folklore application as preventive remedy as an anti-oxidant. The present study shows that Ficus benghalensis leaves have radical scavenging activity and can be considered as good sources of natural anti-oxidants for medicinal and commercial uses.
Keywords: Ficus benghalensis, anti-oxidant, DPPH scavenging activity, Folin-ciocalteu reagent.
2. Uma B, Prabhakar K. In vitro antimicrobial activity and phytochemical analysis of Ficus religiosa and Ficus benghalensis against diarrhoeal enterotoxigenic E. Coli. Ethnobotanical leaflets 2009; 13:472-4.
3. Cerutti PA. Oxidant stress and carcinogenesis. Eur. J. Clin. Invest 1991; 21:1-11.
4. Muhammad AA, Ayesha Z, Tauheeda R, Aziz-ur-R, Samina A, Durre S, Muhammad J, Sabahat Z S, Tayyaba S, Muhammad A. Evaluation of comparative antioxidant potential of Aqueous and organic fractions of Ipomoea carnea. Journal of Medicinal Plants Research 2010; 4(18):1883-7.
5. Halliwel B, Gutteridge JMC. Free radicals in biology and medicine. London: Oxford University Press 1998.
6. Kinsella JE, Frankel E, German B, Kanner J. Possible mechanisms for the protective role of antioxidants in wine and plant foods. Food Technol 1993; 47:85-9.
7. Aswar M, Aswar U, Wagh A, Watkar B, Vyas M, Gujar KN. Antimicrobial Activity of Ficus benghalensis. Pharmacologyonline 2008; 2:715-28.
8. Ahmad S, Rao H, Akhtar M, Ahmad I, Hayat MM, Iqbal Z, Rahmanur N. Phytochemical composition and Pharmacological prospectus of Ficus benghalensis Linn. (Moraceae)-A review. Journal of Medicinal Plants Research 2011; 5(28):393-6400.
9. Bhalerao AS, Poojari CA, Sharma SA. Ethnobotany, Phytochemical Properties and Pharmacological Review of Ficus benghalensis Linn. World Journal of Pharmacy and Pharmaceutical Sciences 2015; 4(08):372-81.
10. Gupta VK, Sharma SK. In vitro antioxidant activities of aqueous extract of Ficus benghalensis Linn. Root. Int. J. Bio. Chem 2010; 4(03):134-40.
11. Narender Boggula. Phytochemical evaluation and in vitro anti bacterial activity of dried seeds of Abrus precatorius. International journal of pharmaceutical sciences review and research 2017; 44(1):101-7.
12. Milan Stanković S. Total phenolic content, flavonoids concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujevac J Sci 2011; 33:63-72.
13. Singleton VL, Rossi Jr JA. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Viticult 1965; 16:144-58.
14. Hatano T, Kagawa H, Yasuhara T, Okuda T. Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects. Chem. Pharm. Bull 1988; 36:1090-2097.
15. Yanala Suresh Reddy, Krishna Mohan Chinnala, K. Vamshi Sharath Nath, E. Madhan Mohan, B. Preethi, B. Narender. In vitro evaluation of anti-oxidant activity of different extracts of Justicia gendarussa leaf. Scholars Research Library, Der Pharma Chemica 2015; 7(8):21-4.
16. Narender Boggula. In vitro evaluation of anti-oxidant activity of different extracts of Aerva lanata leaves. World Journal of Pharmacy and Pharmaceutical Sciences 2016; 5(8):756-61.
17. Pellati F, Benvenuti S, Magro L, Melegari M, Soragni F. Analysis of phenolic compounds and radical scavenging activity of Echinacea spp. J Pharm Biomed Anal. 2004; 35:289-301.
18. Narender Boggula. Evaluation of in vitro anti oxidant activity of dried roots of abrus precatorius. World Journal of Pharmaceutical Research 2017; 6(3):1619-28.
19. Narender Boggula, Himabindu Peddapalli. Phytochemical analysis and evaluation of in vitro anti oxidant activity of Punica granatum leaves. International Journal of Pharmacognosy and Phytochemical Research 2017; 9(8):1110-8.
20. Jao CH, Ko WC. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging by protein hydrolysates from tuna cooking juice. Fishery Sci 2002; 68:430-5.
21. Awika JM, Rooney LW, Wu X, Prior RL. Cisneros zevallos L. Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products. J Agric Food Chem 2003; 51:6657-62.
22. Andarwulan N, Fardiaz D, Wattimena GA, Shetty K. Antioxidant activity associated with lipid and phenolic mobilization during seed germination of Pangium edule Reinw. J. Agric. Food Chem 1999; 47:3158-63.
23. Tsaliki E, Lagouri V, Doxastakis G. Evaluation of the antioxidant activity of lupin seed flour and derivatives (Lupinus albus ssp. Graecus). Food Chem 1999; 65:71-5.
24. Javanmardi J, Stushnoff C, Locke E, Vivanco JM. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chem 2003; 83(4):547-50.
25. Maillard MN, Berset C. Evolution of antioxidant activity during kilning, role of insoluble bound phenolic acids of barley and malt. J Agric Food Chem 1995; 43:1789-93.
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