Extraction and wound healing potential of Nerium Indicum M, Artocarpus Heterophyllus Lam, Murraya Koenigii L, Punica Granatum L on albino rats using burn wound model

  • Renuka Shukla SVN Institute of Pharmaceutical Sciences, Swami Vivekanand University, Sagar, Madhya Pradesh, India
  • Varsha Kashaw SVN Institute of Pharmaceutical Sciences, Swami Vivekanand University, Sagar, Madhya Pradesh, India

Abstract

Objective: The objective of present work was to extract the active constituents and evaluate the wound healing potential of Nerium indicum, Artocarpus heterophyllus, Murraya koenigii, Punica granatum on albino rats using burn wound model. Poly-herbal ointment and gel formulation was developed and compare with the extract of all plants. These plants has shown anti-asthmatic, anti-diabetic, anti-inflammatory, anti-microbial, anti-leukemic, anti-mycobacterial, antibacterial, antioxidant, antiulcer, antiviral, anticancer, antimalarial, anti-inflammatory and wound healing properties. Materials and Methods: Solvent extraction method has been utilized for the separation of the active constituents of interest by using 70% ethanol as a solvent. Poly-herbal ointment and gel formulations has been prepared by mixing the extract of Nerium Indicum, Artocarpus heterophyllus, Murraya koenigii and Punica granatum in different ratio with the ointment base (10-15%) contains PEG and for gel formulation Carbapol-940 base (10-15%). Burn wound model of albino rats has been utilized for the assessment of wound healing potential of the plant extract as well as developed formulations. Results and Conclusion: The studies on burn wound healing model reveals that F2 poly-herbal ointment formulation show better burn wound healing potential in comparison to all other formulations, F-1 ointment formulation as well as extracts of all plants. There was no mortality observed in the tenure of study. Discussion: These data has indicated that ointment and gel poly-herbal formulation of Nerium Indicum, Artocarpus Heterophyllus, Murraya Koenigii and Punica Granatum has been utilized for wound healing purpose and it is safer for topical application. No toxicity and mortality has been observed during the experimental tenure. 


Keywords: Wound healing, Nerium indicum, Murraya koenigii, Punica granatum, Artocarpus heterophyllus, burn wound model.

Downloads

Download data is not yet available.

Author Biographies

Renuka Shukla, SVN Institute of Pharmaceutical Sciences, Swami Vivekanand University, Sagar, Madhya Pradesh, India

SVN Institute of Pharmaceutical Sciences, Swami Vivekanand University, Sagar, Madhya Pradesh, India

Varsha Kashaw, SVN Institute of Pharmaceutical Sciences, Swami Vivekanand University, Sagar, Madhya Pradesh, India

SVN Institute of Pharmaceutical Sciences, Swami Vivekanand University, Sagar, Madhya Pradesh, India

References

1. Kumar S, Sripriya R, Raghavan VH. Wound healing potential of Cassia fistula on infected albino rat model, Journal of Surgical Research 2006; 23:283-89.
2. Rashmi Shukla, Varsha Kashaw. Wound healing prospective of Pongamia glabra, Piper nigrum and Momordica charantia on albino rats using anemic burn wound model. Journal of Drug Delivery & Therapeutics. 2018; 8(6-s):146-154.
3. Enoch S, John LD. Basic Science of wound healing. Ethnopharmacology. 2005; 23:37-42.
4. Guo S, Dipietro LA. Factors affecting wound healing. Journal of Dental Research. 2010 89(3):219-229.
5. Rashmi Shukla, Varsha Kashaw. Evaluation of wound healing prospective of Momordica charantia, Pongamia glabra and on anemic albino rats using incision wound model Piper nigrum. Asian Journal of Pharmacy and Pharmacology. 2019; 5(2):401-408.
6. Nagargoje AN, Phad SS. A Review on phytochemistry and pharmacology of Nerium indicum Mill. Plant. International Journal of Pharmaceutical Sciences Review. 2003; 21(2):148-151.
7. Dey P, Chaudhuri TK. Pharmacological aspects of Nerium indicum Mill, A comprehensive review. Pharmacognosy Review 2014; 8(16):156-162.
8. Bnouham M, Mekhfi H, Legssyer A, Ziyyat A. Medicinal plants used in the treatment of diabetes in Morocco. Int J Diabetes Metab. 2002; 10:33–50.
9. Tantiado RG. Survey on ethnopharmacology of medicinal plants in Iloilo, Philippines. Int J Biosci Biotechnol. 2012; 4:11-26.
10. Tahraoui A, El-Hilaly J, Israili ZH, Lyoussi B. Ethnopharmacological survey of plants used in the traditional treatment of hypertension and diabetes in south-eastern Morocco (Errachidia province). J Ethnopharmacol. 2007; 110:105–17. [PubMed: 17052873]
11. Elevitch CE, Manner HI. Artocarpus heterophyllus (jackfruit). Species Profiles for Pacific Island Agroforestry 2006; 10: 1-25.
12. Akhilhari H, Revikumar KG, Divya D. Artocarpus. A Review of its Phyto-chemistry and Pharmacology. Journal of Pharma Search. 2014; 9(1):7-13.
13. Mohammed HPM, Rashid K, Senthil CK. Artrocarpus Heterophyllus- review study on Potential Activity. Journal of Tropical Biology and Conservation. 2018; 15: 61-80.
14. Burci LM, Silva CB, Rondon JN, Silva LM, Sergio FA, Miguel OG, Dias JFG, Miguel MD. Acute and subacute (28 days) toxicity, hemolytic and cytotoxic effect of Artocarpus heterophyllus seed extracts. 2018. Toxicology Reports. https,//doi.org/10.1016 /j.toxrep.2018.02.006.
15. Baliga MS, Shivshankara AR, Haniadka R, Dsouza J, Bhat HP. Phytochemistry, nutritional and pharmacological properties of Artocarpus heterophyllus Lam (jackfruit). A review. Food Research International. 2011; 44 (7):1800-1811.
16. Abu Bakar MF, Abdul Karim F, Perisamy E. Comparison of phytochemicals and antioxidant properties of different fruit parts of selected Artocarpus species from Sabah, Malaysia. Sains Malays. 2015; 44(3):355–363.
17. Ojwang RA, Muge EK, Mbatia BN, Mwanza BK, Ogoyi DO. Compositional, elemental, phytochemical and antioxidant characterization of Jackfruit (Artocarpus heterophyllus) Pulps and Seeds from Selected Regions in Kenya and Uganda. European Journal of Medical physics. 2018; 23(3):1-12.
18. Shirote PJ, Deepali SS, Thorat SS. Isolation, phytochemical screening and biological evaluation of Punica granatum leaves extract. European Journal of Pharmaceutical and Medical Research. 2018; 5(7):271-275.
19. Sangeetha R, Jayaprakash A. Phytochemical Screening of Punica granatum Linn. Peel Extracts. Journal of Academic and Industrial Research. 2015; 4:160-162.
20. Mansourian A, Boojarpour N, Ashnagar S, Bejtollahi JM, Shamshiri AR. The comparative study of antifungal activity of Syzygium aromaticum, Punica granatum and nystatin on Candida albicans-an in-vitro study. 2014; 24(4):163-168.
21. Li Y, Yang F, Zheng W. Punica granatum (pomegranate) leaves extract induces apoptosis through mitochondrial intrinsic pathway and inhibits migration and invasion in nonsmall cell lung cancer in vitro. Biomed Pharmacother. 2016; 80:227-35.
22. Ali Redha AA, Hasan AM, Mandeel Q. Phytochemical Determinations of Pomegranate (Punica granatum) Rind and Aril Extracts and their Antioxidant, Antidiabetic and Antibacterial Activity. Nat Prod Chem Res. 2018; 64:15-20.
23. Amer OSO, Dkhil MA, Hikal WM, Al Quraishy S. Antioxidant and Anti-Inflammatory Activities of Pomegranate (Punica granatum) on Eimeria papillata-Induced Infection in Mice. Bio Med Research International. 2015; 2015:219670.
24. Singh S, More PK, Mohan SM. Curry leaves (Murraya koenigii Linn. Sprengal )-a miracle plant. Indian Journal of Scientific Research. 2014; 4:46-52.
25. Rajendran MP, Pallaiyan BB, Selvaraj N. Chemical composition, antibacterial and antioxidant profile of essential oil from Murraya koenigii (L.) leaves. Avicenna J Phytomed. 2014; 4(3):200-214.
26. Jadhav VS, Ghawate VB. Evaluation of combined wound healing activity of ethanolic extracts of leaves of Murraya koenigii and Nyctanthes arbortristis on rats. Drug Invention Today. 2017; 9(2): 24-27.
27. Shubhangi ES, Monali DT. Formulation and evaluation of herbal ointment containing Neem and Turmeric extract. Journal of Scientific and Innovative Research. 2016; 5(4):149-151.
28. Asija R, Dhaker PC, Nema N. Formulation & evaluation of voriconazole ointment for topical delivery. Journal of Drug Discovery and Therapeutics. 2015; 26(3):7-14.
29. Sawant SE, Tajane MD. Formulation and evaluation of herbal ointment containing Neem and Turmeric extract. Journal of Scientific and Innovative Research. 2016; 5(4):149-151.
30. Mishra NN, Kesharwani A, Agarwal A, Polachira SK, Nair R, Gupta SK. Herbal Gel Formulation Developed for Anti-Human Immunodeficiency Virus (HIV)-1 Activity Also Inhibits In-vitro HSV-2 Infection. Viruses. 2018; 2410(11):E580. doi: 10.3390/v10110580.
31. Shahtalebi MA, Asghari GR, Rahmani F, Shafiee F, Jahanian-Najafabadi A. Formulation of Herbal Gel of Antirrhinum majus Extract and Evaluation of its Anti-Propionibacterium acne Effects. Adv Biomed Res. 2018; 7:53. doi: 10.4103/abr.abr_9_17. eCollection 2018.
32. Aslani, A., Zolfaghari, B., & Fereidani, Y. Design, formulation, and evaluation of a herbal gel contains melissa, sumac, licorice, rosemary, and geranium for treatment of recurrent labial herpes infections. Dental Research Journal. 2018; 15(3):191-200.
33. Nakae H, Inaba H. Effectiveness of electrolyzed oxidized water irrigation in a burn-wound infection model. J Trauma. 2000; 49:511–514.
34. Pawar RSP, Chourasiya PK, Rajak H, Singour PK, Toppo FA, Jain A, Wound healing activity of Sida cordifolia Linn. in rats, 2013; 45(5): 474-478.
35. Woessner JF., Jr The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Arch Biochem Biophys. 1961; 93:440–447.
36. Gnanamani A, Priya KS, Radhakrishnan N, Babu M. Antibacterial activity of two plant ex-tracts on eight burn pathogens. J Ethnophar-macol. 2003; 86: 59-61.
37. Moyer KE, Saggers GC, Allison GM, Mackay DR. Ehrlich HP. Effects of interleukin-8 on granulation tissue maturation, J. Cell. Physiol. 2002; 193(2):173-179.
38. Chmit M, Kanaan H, Habib J, Abbass M, McHeik A, Chokr A. Antibacterial and antibiofilm activities of polysaccharides, essential oil, and fatty oil extracted from Laurus nobilis growing in Lebanon. Asian Pac. J. Trop. Med. 2014; 7S1: S546-S552.
39. Tabanca N, Avonto C, Wang M, Parcher A. Ali, Demirci B, Raman V, Khan IA. Comparative investigation of Umbellularia californica and Laurus nobilis leaf essential oils and identification of constituents active against Aedes aegypti. Journal of Agriculture Food Chemistry. 2013; 61(50):12283-12291.
Statistics
111 Views | 136 Downloads
How to Cite
Shukla, R., & Kashaw, V. (2019). Extraction and wound healing potential of Nerium Indicum M, Artocarpus Heterophyllus Lam, Murraya Koenigii L, Punica Granatum L on albino rats using burn wound model. Journal of Drug Delivery and Therapeutics, 9(1-s), 337-346. https://doi.org/10.22270/jddt.v9i1-s.2360

Most read articles by the same author(s)