Extraction, Qualitative and Quantitative Determination of Secondary Metabolites of Aerial Parts of Clematis heynei and Solanum virginianum

  • Pravin Morankar SMBT College of Pharmacy, Ghoti-Sinnar Road, Dhamangaon, Maharashtra 422403
  • Alok Pal Jain Sarvepalli Radhakrishnan University, NH-12 Hoshangabad Road, Misrod, Bhopal, Madhya Pradesh 462026

Abstract

The increasing interest in powerful biological activity of secondary metabolites outlined the necessity of determining their contents in medicinal plants. In the last few years, there has been an exponential growth in the field of herbal medicine and gaining popularity both in developing and developed countries because of their natural origin and less side effects. Clematis heynei (C. heynei, Ranunculaceae) is commonly known as Deccan clematis, Murhar, Morvel, Ranjaee and it is a somewhat woody climber very sparsely distributed in deciduous forests of Western Ghats, India. In the Indian system of medicine ‘Ayurveda’ this plant is used to eliminate malarial fever and headache. Different plant parts were used for treating various diseases. Solanum virginianum L. (S. virginianum, Solanaceae, Solanum xanthocarpum Schrad. & H. Wendl.) is a diffuse and very prickly under shrub. It is found growing commonly in various regions of the world on sandy soils and is distributed throughout India. The plant is used traditionally to treat asthma, chest pain, leucoderma, scorpion bite, and sterility in women. The aim of the present study is to examine C. heynei and S. virginianum aerial parts of plant for phytochemical profile. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids, fixed oil and fats. The total phenolics content of methanolic and aqueous extract of C. heynei was (0.592, 0.292 mg/100mg), followed by flavonoids (1.371, 0.723mg/100mg) respectively. The total phenolics content of methanolic extract of S. virginianum was (0.345mg/100mg), followed by flavonoids (0.978mg/100mg). The present study concluded that the crude extract of C. heynei and S. virginianum is a rich source of secondary phytoconstituents which impart significant antioxidant potential. The findings of the present study will be helpful to phytochemists, pharmacologists and pharmaceutical industries.


 

Downloads

Download data is not yet available.

Author Biographies

Pravin Morankar, SMBT College of Pharmacy, Ghoti-Sinnar Road, Dhamangaon, Maharashtra 422403

SMBT College of Pharmacy, Ghoti-Sinnar Road, Dhamangaon, Maharashtra 422403

Alok Pal Jain, Sarvepalli Radhakrishnan University, NH-12 Hoshangabad Road, Misrod, Bhopal, Madhya Pradesh 462026

Sarvepalli Radhakrishnan University, NH-12 Hoshangabad Road, Misrod, Bhopal, Madhya Pradesh 462026

References

1. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999; 12:564 82.
2. Mradu G, Saumyakanti S, Sohini M, Arup M. HPLC Profiles of Standard Phenolic Compounds Present in Medicinal Plants. Int J Pharmacog Phytochem Res 2012; 4: 162-167.
3. Nwokocha A, Blessing IO, Agabagwa, Okoli BE. Comparative phytochemical screening of Jatropha L. species in the Niger Delta. Res J Phytochem 2011; 5:107-14.
4. Majaw S, Moirangthem J. Qualitative and quantitative analysis of Clerodendron colebrookianum Walp. Leaves and Zingiber cassumunar Roxb. Rhizomes. Ethnobotanical leaflet 2009; 13:578-89.
5. Njoku PC, Akumefula MI. Phytochemical and nutrient evaluation of Spondias mombin leaves. Pak J Nutri 2007; 6:613 5.
6. Linga Rao M, Savithramma N. Quantification of primary and secondary metabolites of Svensonia hyderobadensis– A rare medicinal plant. Int J Phar Phram Sci 2012; 4:519 21.
7. Rita P, Animesh DK. An updated overview on Solanum xanthocarpum Schrad and Wendl. Int J Res Ayurveda Pharm 2011;2(3):730-5.
8. Reddy NM, Reddy RN. Solanum xanthocarpum chemical constituents and medicinal properties: A review. Sch Acad J Pharm 2014;3(2):146-9.
9. Kayani S, Ahmad M, Zafar M, Sultana S, Khan MP, Ashraf MA, et al. Ethnobotanical uses of medicinal plants for respiratory disorders among the inhabitants of Gallies - Abbottabad, Northern Pakistan. J Ethnopharmacol 2014;156:47-60
10. Kumar PR. Ethno medicinal plants used for oral health care in India. Int J Herb Med 2014;2(1):81-7.
11. Rana S, Prakash V, Sagar A. Antibacterial activity of Solanum xanthocarpum leaf extract. Int J Curr Microbiol Appl Sci 2016;5(4):323-8.
12. Prashith Kekuda TR, Raghavendra HL, Rajesh MR, Avinash HC, Ankith GN, Karthik KN. Antimicrobial, insecticidal and antiradical activity of solanum virginianum l. (solanaceae). Asian J Pharm Clin Res 2017; 10(11):163-167.
13. Mahekar PD, Yadav SR. Medicinal plants of South Western Maharashtra. Biodiversity of India (Ed. - T. Pullaiah) 2006; 4: 75-563.
14. Devarkar VD. Plants of great medicinal value from korku ethnomedicine. Biosci Disc 2010; l (1): 21-25,.
15. Song ZH, Zhao YY, Duan JL, Wang X. Review of chemical constituents and pharmacological actions of Clematis species. Nat Prod Res Dev1996; 7:67-72.
16. Liu LF, Ma XL, Wang YX, Li FW, Li YM, Wan ZQ, Tang QL. Triterpenoid saponins from the roots of Clematis chinensis Osbeck. J Asian Nat Prod Res 2009;11:389-396.
17. Huang WW. Advances in studies on chemical constituents and pharmacological effect of Clematis L. Chinese Trad Herb Drug 2002;33:285-290.
18. Zhi ZD, Xian WY, Hao H, Xiang HC, Xiao DL A new flavone C-glycoside from Clematis rehderiana. Molecules 2010; 15:672-679.
19. Thapliyal RP, Bahuguna RP. Clemontanoside-275 C, a saponin from Clematis montana. Phytochemistry1993; 33:671-673.
20. Kawata YH Kizu Y, Miyaichi T, Tomimori L. Studies on the constituents of Clematis species. VIII. Triterpenoid saponins from the aerial part of Clematis tibetana KUNTZ. Chem Pharma Bull 2001; 49:635-638.
21. Southwell IA, Tucker DJ. Protoanemonin in Australian Clematis. Phytochemistry1993; 33:1099-1102.
22. Mukherjee PK. Quality control of herbal drugs. 2nd Ed. Business Horizons; 2007.
23. Khandelwal KR Practical pharmacognosy technique and experiments. 23rd Ed. Nirali Prakashan; 2005.
24. Kokate CK. Practical pharmacognosy. 4th Ed. Vallabh Prakashan; 1994.
25. Olufunmiso OO, Afolayan AJ. Phenolic content and antioxidant property of the bark extract of Ziziphus mucronata willd. Subsp. mucronata willd, BMC Complement Alternative Medicine 2011; 11:130.
Statistics
103 Views | 140 Downloads
How to Cite
Morankar, P., & Jain, A. P. (2019). Extraction, Qualitative and Quantitative Determination of Secondary Metabolites of Aerial Parts of Clematis heynei and Solanum virginianum. Journal of Drug Delivery and Therapeutics, 9(1-s), 260-264. https://doi.org/10.22270/jddt.v9i1-s.2346