Polyherbal Formulation Concept for Synergic Action: A Review
Formulations restrain 2 or more than 2 herbs are called polyherbal formulation. Drug formulation in Ayurveda is based on 2 principles: Use as a single drug and use of more than one drug. The last is known as polyherbal formulation. The idea of polyherbalism is peculiar to Ayurveda even though it is tricky to explain in term of modern parameters. The Ayurvedic literature Sarangdhar Samhita tinted the idea of polyherbalism to attain greater therapeutic efficacy. Polyherbal formulation has been used all around the earth due to its medicinal and therapeutic application. It has also recognized as polyherbal therapy or herb-herb combination. The active phytochemical constituents of individual plants are inadequate to attain the desirable therapeutic effects. When polyherbal and herbo-mineral formulations combining the multiple herbs in a meticulous ratio, it will give an enhanced therapeutic effect and decrease the toxicity. The active constituents used from individual plant are inadequate to provide attractive pharmacological action. There are evidences that crude plant extracts often have greater potency rather than isolated constituents. In traditional medicine whole plants or mixtures of plants are used rather than isolated compounds. Due to synergism, polyherbalism confers some benefits which are not accessible in single herbal formulations. Polyherbal formulations express high effectiveness in numerous diseases with safe high dose. Based on the nature of the interaction, there are 2 mechanisms on how synergism acts (i.e., pharmacodynamics and pharmacokinetic). In words of pharmacokinetic synergism, the capacity of herb to ease the absorption, distribution, metabolism and elimination of the other herbs is focused. Pharmacodynamics synergism on the other hand, studies the synergistic effect when active constituents with similar therapeutic activity are targeted by diverse mechanism of action. The present review encompasses all the significant features of polyherbal formulation.
Keywords: Polyherbal formulation, Ayurveda, Active constituents, Pharmacodynamics, pharmacokinetic
2. Farnsworth NR, Akerele O, Bingle AS, Sojarto DD, Guo Z. Medicinal plant in therapy. Bulletin of the world health organization. 1985; 63:965-981.
3. Abraham R, Paridhavi M. A review of comprehensive study on medicinal plants of polyherbal formulation- Churna. Asian J Pharm Clin Res 2013; 6(4):11-18.
4. Kunwar RM, Shrestha KP, Bussmann RW. Traditional herbal medicine in far-west Nepal: a pharmacological appraisal. J Ethnobiol Ethnomed 2010; 6:35.
5. Ngo LT, Okogun JI, Folk WR. 21st century natural product research and drug development and traditional medicines. Nat Prod Rep 2014; 30(4):584-92.
6. Che CT, Wang ZJ, Chow MSS, Lam CWK. Herb-herb combination for therapeutic enhancement and advancement: Theory, practice and future perspectives. Molecules. 2013; 18(5):5125–41.
7. Risberg K, Fodstad Ø, Andersson Y. Synergistic anticancer effects of the 9.2.27PE immunotoxin and ABT-737 in melanoma. PLoS One 2011;6 (9)
8. Ramaiah M, Chakravathi G, Yasaswini K. In vitro biological standardization, formulation and evaluation of directly compressed polyherbal anthelmintic tablets. Pharmacogn J 2013; 5(3):130-4.
9. Ajazuddin, Saraf S. Evaluation of physicochemical and phytochemical properties of Safoof-E-Sana, a Unani polyherbal formulation. Pharmacognosy. Res 2010; 2(5):318–22.
10. Reddy MS, Reddy DRK, Prasad NAV. Herbal and pharmaceutical drugs enhanced with probiotics. 2000, US006080401A
11. Bhope SG, Nagore DH, Kuber VV, Gupta PK, Patil MJ. Design and development of a stable polyherbal formulation based on the results of compatibility studies, Pharmacognosy Res. 2011; 3(2): 122-129.
12. Benzie IFF, Wachtel-Galor S. Herbal Medicine: Bimolecular and Clinical Aspects, 2nd edn. CRC Press/Taylor & Francis 2011.
13. Spinella M. The importance of pharmacological synergy in psychoactive herbal medicines. Altern Med Rev. 2002; 7:130-137.
14. Chorgade MS. Drug Discovery and Development: Drug development Vol. 2. John Wiley and Sons Inc; Hoboken, New Jersey: 2007.
15. Parasuraman S, Thing GS, Dhanaraj SA. Polyherbal formulation: concept of ayurveda Pharmacogn Rev 2014; 8(16): 73-80.
16. Sarwar M, Attitalla IH and Abdollahi M. A review on the recent advances in pharmacological studies on medicinal plants: Animal studies are done but clinical studies needs completing. Asian J Anim Vet Adv 2011; 6(8): 867-883.
17. Kavitha AN, Deepthi V, Nayeem N. Design, formulation and evaluation of a polyherbal ointment for its wound healing activity. Pharmacophore 2013; 4 (5):175-180.
18. Munshi R, Bhalerao S, Kalekar S. Proceedings. J Ayurveda Integr Med 2012; 3:168 72. 59.
19. Rastogi S, Chiappelli F, Ramchandani MH, Singh RH, editors. Evidence based Practice in Complimentary and Alternative Medicine Perspective, Protocols, Problems and Potential in Ayurveda. New York City: Springer; 2012.
20. Lee H-S, Lee B-C, Ku S-K Effect of DHU001, a Polyherbal Formula, on Dinitrofluorobenzene-induced Contact Dermatitis (Type I allergy), Toxicol Res. 2010; 26(2):123-130.
21. Zeng KW, Zhang T, Fu H, Liu GX, Wang XM. Modified Wu-Zi-Yan-Zong prescription, a traditional Chinese polyherbal formula, suppresses lipopolysaccharide-induced neuroinflammatory processes in rat astrocytes via NF-κB and JNK/p38 MAPK signaling pathways. Phytomedicine. 2012 15; 19(2):122-129.
22. Yang Z, Grinchuk V, Ip SP, Che C-T, Fong HHS, Lao L, et al. Anti-Inflammatory Activities of a Chinese Herbal Formula IBS-20 In Vitro and In Vivo. Evid Based Complement Alternat Med 2012; 2012:1–12.
23. Fulzele SV, Satturwar PM, Joshi SB, Dorle AK. Studies on anti-inflammatory activity of a polyherbal formulation - Jatyadi ghrita. Indian Drugs 2002; 39:42-4.
24. Tripathi YB, Reddy MM, Pandey RS, Subhashini J, Tiwari OP, Singh BK, Reddanna P. Anti-inflammatory properties of BHUx, a polyherbal formulation to prevent atherosclerosis, Inflammopharmacology 2004; 12(2):131-152.
25. Medeiros KCP, Monteiro JC, Diniz MFFM, Medeiros IA, Silva BA, Piuvezam MR .Effect of the activity of the Brazilian polyherbal formulation: Eucalyptus globulus Labill, Peltodon radicans Pohl and Schinus terebinthifolius Radd in inflammatory models, Brazilian Journal of Pharmacognosy 2007; 17(1):23-28.
26. Deorukhakar SR, Dethe A, Vohra RR, Ghosh R, Chaudhary J, Kadam V Antiinflammatory Activity of a Polyherbal Formulation, Indian J Pharm Sci. 2008; 70(6):785-787.
27. Rasool M, Sabina EP. Anti-inflammatory effect of the Indian Ayurvedic herbal formulation Triphala on adjuvant-induced arthritis in mice, Phytother Res. 2007; 21(9):889-894.
28. Abdul L, Abdul R, Sukul RR, Nazish S. Anti-inflammatory and Antihistaminic Study of a Unani Eye Drop Formulation, Ophthalmol Eye Dis. 2010; 2: 17-22.
29. Jung KH, Choi HL, Park S, Lee G, Kim M, Min JK, Min BI, Bae H..The effects of the standardized herbal formula PM014 on pulmonary inflammation and airway responsiveness in a murine model of cockroach allergen-induced asthma, J Ethnopharmacol. 2014; 155(1):113-22.
30. Asad M, Prasad K, Thomas L, Kamath JV. Evaluation of anti-arthritic and anti-inflammatory activity of sudard, a poly herbal formulation. Iranian Journal of Pharmacology & Therapeutics 2007; 6(1):71-75.
31. Khanna N, Sharma SB. Anti-inflammatory and analgesic effect of herbal preparation: Septilin. Indian J Med Sci 2000; 55(4):195-202.
32. Kweifio-Okai G. Anti-inflammatory activity of a Ghanaian antiarthritic herbal preparation, J Ethnopharmacol, 1991;33(3):263-267s
33. Jagtap AG, Shirke SS, Phadke AS. Effect of polyherbal formulation on experimental models of inflammatory bowel diseases J Ethnopharmacol. 2004; 90(2-3):195-204.
34. Aswatha Ram HN, Sriwastava NK, Makhija IK, Shreedhara CS. Anti-inflammatory activity of Ajmodadi churna extract against acute inflammation in rats, J Ayurveda Integr Med. 2012; 3(1):33-37.
35. Senthilvet G., Jegadeesan M., Austin A., Thirugnanasambanthan P., Mayisurem E., Balasubramanian M., Narayanan N. and Viswanathan S. Effect of polyherbal formulation (Diarun plus) on streptozotocin induced experimental diabetes, Int J Tropical Med 2006; 1 (2):88-92,
36. Qadri NM, Zakir-ur-Rehman, Rizvi HA. Evaluation of herbal drug for hypoglyce¬mic activity in normal, hyperglycemic and alloxan-induced diabetic rabbits. Pakistan J Chem. 2011; 1(1):32-37.
37. Ogbonnia SO, Mbaka GO, Adekunle A, Anyika EN, Gbolade OE. Effect of a poly-herbal formulation Okudiabet on alloxan- induced diabetic rats Agriculture and Biology Journal of North America 2010; 1(2):139-145
38. Ghorbani A, Shafiee-Nick R, Rakhshandeh H, Borji A. Antihyperlipidemic effect of a polyherbal mixture in streptozotocin-induced diabetic rats. J Lipids 2013; 2013:675759.
39. Madkour FF, Hassan MM, Khalil WF, AbdoW. Wound Healing Activity of Brown Algae plus Polyherbal Extract in Normal and Alloxan-induced Diabetic Rats. J Adv Veter Res 2013; 3:102-108.
40. Sayeed SR, Ahmed H, Rahman S, Ahmad I, Rahman M, Hossan S, Rahmatullah M. Polyherbal formulation for lowering blood glucose levels: evaluation of a combination of foeniculum vulgare and brassica alba seeds. World Journal of Pharmacy and Pharmaceutical Sciences 2015; 4(10):79-85.
41. Latha P, Srikanth A, Ashok kumar CK, Geethanjali B, Janaki Prasanna S, Swathi M, Priyanka DS. Evaluation of antidiabetic potential of marketed polyherbal formulation (ayurslim) on high fat diet-streptozotocin induced obese diabetic rats, International Journal of Biological & Pharmaceutical Research. 2012; 3(4):524-530.
42. Subhasree N, Kamella AK, Kaliappan I, Agrawal A, Dubey GP. Antidiabetic and antihyperlipidemic activities of a novel polyherbal formulation in high fat diet/streptozotocin induced diabetic rat model, Indian J Pharmacol 2015; 47(5):509-513.
43. Gauttam VK, Kalia AN. Development of polyherbal antidiabetic formulation encapsulated in the phospholipids vesicle system, J Adv Pharm Technol Res. 2013; 4(2):108-117.
44. Peterson CT, Denniston K, Chopra D.Therapeutic uses of Triphala in Ayurvedic medicine. J Altern Complement Med 2017; 23(8): 607-614.
45. Saravanan R and Pari L. antihyperlipidemic and antiperoxidative effect of diasulin, a Polyherbal formulation in alloxan induced hyperglycemic rats, BMC complementary and Alternative medicine, 2005; 5:14.
46. Patel SS, Shah RS, Goyal RK. Antihyperglycemic, antihyperlipidemic and antioxidant effect of Dihar, a polyherbal ayurvedic formulation in streptozotocin induced diabetic rates, Indian J Exp Biol 2009; 47:564-570.
47. Balaji V, Williams AA, Sathish S, Mayilvanan C, Narasimhan A, Balasubramanian K. Antidiabetic Potentials of a Novel Polyherbal Preparation Formulated According to Principles of Siddha System of Medicine. Journal of evidence-based complementary & alternative medicine, 2013; 18 (1):29-42.
48. Jung HW, Jung JK, Ramalingam M, Yoon C-H, Bae HS, Park Y-K. Anti-diabetic effect of Wen-pi-tang-Hab-Wu-ling-san extract in streptozotocin-induced diabetic rats. Indian J pharmacol 2012; 44(1):97-102.
49. Hu W, Yeo J-H, Jiang Y, Heo S Wang M-H. The antidiabetic effects of an herbal formula composed of Alnus hirsuta, Rosa davurica, Acanthopanax senticosus and Panax schinseng in the streptozotocin-induced diabetic rats. Nutr Res Pract 2013;7(2):103-108.
50. Jain S, Pandhi P, Singh AP, Malhotra S. Efficacy of standardised herbal extracts in type 1 diabetes - an experimental study. Afr J Trad CAM 2006; 3(4):23-33.
51. Tatke PA, Nidhiya ISR, Deshpande SG, Safety profile of a polyherbal formulation (Gynocare capsules) in female rats by subchronic oral toxicity study. Toxicol Int 2012; 19(2):106-111
52. Akhtar MS, Zafar M, Irfan HM, Bashir S. Hypoglycemic effect of a compound herbal formulation (Ziabeen) on blood glucose in normal and alloxan-diabetic rabbits. Diabetologia Croatica 2012; 41-3:87-94.
53. Sharma PR, Laware SL. in-vitro antidiabetic effect of polyherbal formulation, Indo American Journal of Pharm Research2015; 5(06):2402-2407.
54. Baldi A, Goyal S. Hypoglycemic effect of polyherbal formulation in alloxan induced diabetic rats, Pharmacologyonline 2011; 3:764-773.
55. Bhattacharya SK, Satyan KS, Chakrabarti A. effect of Trasina, an ayurvedic herbal formulation, on pancreatic islet superoxide dismutase activity in hyperglycaemic rats. Indian J Exp Biol 1997; 35(3):297-9.
56. Petchi RR, Vijaya C, Parasuraman S. Antidiabetic activity of polyherbal formulation in Streptozotocin- nicotinamide induced diabetic wistar rats. J Tradit Complement Med 2014; 4(2):108–117.
57. Babu PS, Stanely Mainzen Prince P. Antihyperglycaemic and antioxidant effect of hyponidd, an ayurvedic herbomineral formulation in streptozotocin-induced diabetic rats. J Pharm Pharmacol. 2004; 56(11):1435-42.
58. Pari L, Saravanan G. Antidiabetic effect of cogent db, an herbal drug in alloxan-induced diabetes mellitus. Comp Biochem Physiol C Toxicol Pharmacol 2002; 131(1):19-25.
59. Saravanan R, Pari L. Antihyperlipidemic and antiperoxidative Effect of Diasulin, a polyherbal formulation in alloxan induced Hyperglycemic rats. Bmc complement Altern Med. 2005; 22:5:14.
60. Chang MS, Oh MS, Kim DR, Jung KJ, Park S, Choi SB, Ko BS, Park SK. Effects of okchun-san, an herbal formulation, on blood glucose levels and body weight in a model of type 2 diabetes. J ethnopharmacol 2006; 103(3):491-495.
61. Mandlik RV, Desai SK, Naik SR, Sharma G, Kohli RK. Antidiabetic activity of a polyherbal formulation (drf/Ay/5001). Indian J Exp Biol 2008; 46(8):599-606.
62. Yadav H, Jain S, Prasad GB, Yadav M. Preventive effect of Diabegon, a polyherbal preparation, during progression of Diabetes induced by high-fructose feeding in rats. J Pharmacol Sci 2007; 105(1):12-21.
63. Thakkar NV, Patel JA. Pharmacological evaluation of Glyoherb A polyherbal formulation on streptozotocin-induced diabetic Rats. Int J Diabetes Dev Ctries 2010; 30(1):1-7.
64. Yadav D, Chaudhary AA, Garg V, Anwar MF, Rahman MM, Jamil SS, Khan HA, Asif M. In vitro toxicity and antidiabetic Activity of a newly developed polyherbal formulation (mac-st/001) in streptozotocin-induced diabetic wistar Rats. Protoplasma. 2013; 250(3):741-749.
65. Kesavanarayanan KS, Sathiya S, Kalaivani P, Ranju V, Sunil AG, Saravana Babu C, Kavimani S, Prathiba D. Dia-2, A polyherbal formulation ameliorates hyperglycemia and Protein-oxidation without increasing the body weight in Type ii diabetic rats. Eur Rev Med Pharmacol Sci. 2013; 17(3):356-369.
66. Chan JY, Lam FC, Leung PC, Che CT, Fung KP. Antihyperglycemic And antioxidative effects of a herbal formulation Of radix astragali, radix codonopsis and cortex lycii in A mouse model of type 2 diabetes mellitus. Phytother res. 2009; 23(5): 658-665.
67. Joshi CS, Priya ES, Venkataraman S. Hypoglycemic and Antilipidperoxidative effects of a polyherbal formulation, Diakyur, in experimental animal models. J Health Sci 2007; 53(6):734-739.
68. Bangar OP, Earald EE, Asghar S, Ahmad S. Antidiabetic Activity of a polyherbal formulation (karnim plus). Int J Green Pharm 2009; 3(3):211-214.
69. Katiyar DK, Mehrotra A, Pant KK. Hypoglycemic and antioxidant effects of a polyherbal formulation in alloxan Induced diabetic rats. Ann ayurvedic med 2012; 1(4):134-139.
70. Lanjhiyana S, Garabadu D, Ahirwar D, Rana CA, Ahirwar B, Lanjhiyana SK. Pharmacognostic standardization and Hypoglycemic evaluations of novel polyherbal formulations. Der Pharm Lett 2011; 3(1):319-333.
71. Khan AI, Balaji V, Williams AA, SathishS, Mayilvanan C, Balasubramanian K. A novel polyherbal preparation for the management of type-2 diabetes mellitus: a clinical Study. Int J Pharm Pharm Sci 2012; 4(3):495-500.
72. Kant S, Sahu M, Sharma S, Kulkarni KS. Effect of Diabecon (d-400), an ayurvedic herbomineral formulation on diabetic retinopathy. Indian J Clin Pract 2002; 12(9):49-56.
73. Yajnik VH, Acharya HK, Vithlani MP, Yajnik NV. Efficacy and safety of diabecon (d-400), an herbal formulation, in diabetic patients. Indian Pract 1993; 46(12):917-922.
74. Viswanathan V, Kesavan R, Kavitha KV, Kumpatla S. A pilot study on the effects of a polyherbal formulation cream on diabetic foot ulcers. Indian J Med Res 2011; 134:168-173.
75. Said O, Fulder S, Khalil K, Azaizeh H, Kassis E, Saad B. Maintaining a physiological blood glucose level with glucolevel, A combination of four anti-diabetes plants used in the traditional Arab herbal medicine. Evid Based Complement Alternat Med 2008; 5(4):421-428.
76. Pari L, Ramakrishnan R, Venkateswaran S. Antihyperglycaemic effect of Diamed, a herbal formulation, in experimental diabetes in rats, journal of pharmacy and pharmacology J Pharm Pharmacol 2001; 53(8):1139-43.
77. Sateesh B , Veeranjaneyulu A. Toxicological studies on ayurvedic formulation Mersina in albino rates Arch Pharm Sci & Res 2009; 1(2):130-137
78. Guruvayoorappan C, Sudha G. Phytopharmacological evaluation of Byesukar for hypoglycaemic activity and its effect on lipid profile and hepatic enzymes of glucose metabolism in diabetic rats, Ann ohepatol 2008; 7(4):358-363.
79. Bera TK, De D, Chatterjee K, Ali MK, Ghosh D. Effect of Diashis, a polyherbal formulation, in streptozotocin-induced diabetic male albino rats. Int J Ayurveda Res 2010; 1(1):18-24.
80. Amarachinta PR, Jamil K. Preclinical evaluation of polyherbal formulations: hypoglycemic activity in rats. Int J Ayur Herb Med 2012; 2(2):218-228
81. Bhusari VS, Bhusari GS. Effect of Madhumeh an antibiotic polyherbal formulation on carbohydrate metabolism and antioxidant defence in streptozotocin-nicotinamide (stz-nico) induced diabetic rats. Int J Pharm Life Sci. 2012; 3(5):1690-1695.
82. Sengupta K, Mishra AT, Rao MK, Sarma KVS, Krishnaraju AV, Trimurtulu G. Efficacy and tolerability of a novel herbal formulation for weight management in obese subjects: a randomized double blind placebo controlled clinical study. Lipids health Dis 2012; 11: 122.
83. Sruthi T, Satyavati D, Upendar K, Pradeep Kumar C. Antidiabetic activity and anti-oxidant activity of Niddwin, a polyherbal formulation in alloxan induced diabetic rats, Int J Pharm Pharma Sci 2014; 6(2):273-277.
84. Patrick-iwuanyanwu KC, Amadi U, Charles IA, Ayalogu EO. Evaluation of acute and sub-chronic oral toxicity study of baker cleansers bitters-a polyherbal drug on experimental rats. Excli J 2012; 11:632-640.
85. Shalam M, Harish MS, Farhana SA. Prevention of dexamethasone- and fructose-induced insulin resistance in rats by SH-01D, an herbal preparation. Indian J Pharmacol 2006;38(6):419-422
86. Nishteswar K, bhuvad S. Mehahara dashemani-a novel group developed based on sushruta’s mehahara drugs with special referenceto diabetes, Global J Res Med Plants & Indigen Med 2015; 4(6):111-124.
87. Mutalik S, Chetana M, Sulochana B, Devi PU, Udupa N. Effect of Dianex, an herbal formulation on experimentally induced diabetes mellitus. Phytother Res 2005; 19(5):409 -415.
88. Rathore B, Dixit P, Londhe J, Ghaskadbi S, Devasagayam TPA. Recent advances in Indian herbal drug research Guest Editor: Thomas Paul Asir Devasagayam Indian herbal medicines: possible potent therapeutic agents for rheumatoid arthritis. J Clin Biochem Nutr 2007; 41(1):12-17.
89. Gopumadhavan S, Rafiq M, Venkataranganna MV, Mitra SK. Antihistaminic and antianaphylactic activity of HK-07, a herbal formulation, Indian J Pharmacol 2005;37(5):300-303.
90. Won Jung H, Jung JK, Weon CC, Kang JS, Park YK. Antiallergic effect of KOB03, a polyherbal medicine, on mast cell-mediated allergic responses in ovalbumin-induced allergic rhinitis mouse and human mast cells. J Ethnophar¬macol 2012; 142(3):684-693.
91. Abdul L, Abdul R, Sukul RR, Nazish S. Anti-inflammatory and antihistaminic study of a unani eye drop formulation. Ophthalmol Eye Dis 2010; 2:17-22.
92. Gohil PV, Mehta AA. Evaluation of Mast Cell Stabilizing and Anti-Anaphylactic Activity of Polyherbal Formulation, Advances in Biological Research 2011; 5(6):304-308.
93. Kajaria D, Tripathi JS, Tiwari SK., Pandey BL. Anti-histaminic, mast cell stabilizing and bronchodilator effect of hydroalcoholic extract of polyherbal compound-Bharangyadi. Anc sci life. 2012; 31(3): 95-100.
94. Jayaprakasam B, Yang N , Wen M-C , Wang R, Goldfarb J, Sampson H , Li X-M. Constituents of the anti-asthma herbal formula ashmitm synergistically inhibit IL-4 and IL-5 secretion by murine TH2 memory cells and eotaxin by human lung fibroblasts in vitro. J Integrat Med 2013; 11(3): 195-205.
95. Thomas M, Sheran J, Smith N, Fonseca S, Lee AJ. AKL-1, a botanical mixture for the treatment of asthma: a randomised, double-blind, placebo-controlled, cross-over study. BMC Pulm Med. 2007; 7: 4.
96. Wong EL, Sung RY, Leung TF, Wong YO, Li AM, Cheung KL, Wong CK, Fok TF, Leung PC. Randomized, double-blind, placebo-controlled trial of herbal therapy for children with asthma. J Altern Complement Med 2009; 15(10):1091-1097.
97. Srinivasarao D, Jeyraaj IA, Jeyraaj R. Evolution of activity of ‘pentapala 04’ a herbal formulation of the lung lipids against ova albumin and aluminium hydroxide induced lung damage in rats. Ancient Sci Life 2004; 23:9-13.
98. Sengupta K, Kolhapure SA. Evaluation of efficacy and safety of Bresol (HK-07) tablets in upper and lower respiratory tract allergic diseases in children. Medicine Update 2004; 12(3):37-46.
99. Mitra SK, Gopumadhavan S, Venkataranganna MV, Anturlikar SD. Antiasthmatic and antianaphylactic effect of E-721B, a herbal formulation, Indian J Pharmacol 1999; 31:133-137.
100. Kajaria DK, Gangwar M, Sharma AK, Tripathi YB, Tripathi JS, Tiwari S. Evaluation of in vitro antioxidant capacity and reducing potential of polyherbal drug- Bha¬rangyadi. Anc Sci Life 2012; 32(1):24-8.
101. Pluemsamran T, Tripatara P, Phadungrakwittaya R, Akarasereenont P, Laohapand T, Panich U. Redox Mechanisms of AVS022 , an Oriental Polyherbal Formula, and Its Component Herbs in Protection against Induction of Matrix Metalloproteinase-1 in UVA-Irradiated Keratinocyte HaCaT Cells. Evid Based Complement Alternat Med 2013; 2013:739473.
102. Alexandru V, Gaspar A, Savin S, Toma A, Tatia R, Gille E. Phenolic content, antioxidant activity and ef¬fect on collagen synthesis of a traditional wound healing polyherbal formula. University Studies Vasile Goldiş, Life Sciences Series 2015; 25(1):41-6.
103. Arjuman A, Nair V, Gopalakrishna HN , Nandini M. Evaluation of the antioxidant potential of NR-ANX-C (a polyherbal formulation) and its individual constituents in reversing haloperidol-induced catalepsy in mice. Indian J Pharmacol 2007; 39(3):151-154.
104. Mitra SK, Venkataranganna MV, Sundaram R, Gopumadhavan S. Antioxidant activity of AO-8, a herbal formulation in vitro and in vivo experimental models. Phytother Res. 1999; 13(4):300-3.
105. Ghosh A, Kundu A, Seth A, Singh AK, Maurya SK. Preliminary evaluation of hepatoprotective potential of the polyherbal formulation, J Intercult Ethnopharmacol. 2015; 4(2):118-124.
106. Parasuraman S, Kumar EP, Kumar A, Emerson SF. Free radical scavenging property and diuretic effect of Triglize, a polyherbal formulation in experimental models, J Pharmacol Pharmacother 2010; 1(1):38-41.
107. Anandjiwala S, Bagul MS, Parabia M, Rajani M. evaluation of free radical scavenging activity of an ayurvedic formulation, Panchvalkala. Indian J Pharm Sci 2008; 70(1):31-35.
108. Singhal S, Rathore AS, Lohar V, Dave R, Dave J. Pharmacological Evaluation of “Sugar Remedy,” A Polyherbal Formulation, on Streptozotocin-Induced Diabetic Mellitus in Rats. J Tradit Complement Med. 2014 Jul-Sep; 4(3):189-195.
109. Hamid KS, Reza KA, Ranjbar SH, Esfehani MM, Kamalinejad M, Larijani B. A systematic review of the antioxidant, anti-diabetic, and anti-obesity effects and safety of triphala herbal formulation. Journal of Medicinal Plants Research 2013; 7(14):831-844.
110. Samarakoon SM, Chandola HM, Shukla VJ. Evaluation of antioxidant potential of amalakayas rasayana: a polyherbal ayurvedic formulation. Int J Ayurveda Res 2011; 2(1):23-28.
111. Kamtekar S, Keer V, Patil V. Estimation of Phenolic content, Flavonoid content, Antioxidant and Alpha amylase Inhibitory Activity of Marketed Polyherbal Formulation. J AppPharm Sci 2014; 4 (09):061-065.
112. D'Souza P, Amit A, Saxena VS, Bagchi D, Bagchi M, Stohs SJ. Antioxidant properties of aller-7, a novel polyherbal formulation for allergic rhinitis.Drugs Exp Clin Res 2004; 30(3):99-109.
113. Kumar MR, Reddy AG, Anjaneyulu Y, Reddy GD. Oxidative Stress Induced by Lead and Antioxidant Potential of Certain Adaptogens in Poultry. Toxicol Int 2010; 17(2):45–48.
114. Anturlikar SD, Rafiq M, Azeemuddin M, Viswanatha GL, Jagadeesh M, Rao KSS, Patki PS. Free radical scavenging and hepatoprotective activity of HD-03/ES in experimental models, J Exp Inte Med 2012; 2(2):161-166
115. Akhtar MS, Asjad HMM, Bashir S, Malik A, Khalid R, Gulzar F, Irshad N. Evaluation of antioxidant and hepatoprotective effects of khamira Gaozaban Ambri Jadwar Ood Saleeb Wala (KGA). Bangladesh J Pharmacol. 2013; 8:44-48.
116. Aziz N, Mehmood MH, Mandukhal SR, Bashir S, Raoof S, Gilani AH. Antihypertensive, antioxidant, antidyslipidemic and endothelial modulating activities of a polyherbal formulation (POL-10). Vascul Pharmacol 2009; 50(1-2):57-64.
117. Jain DP, Pancholi SS, Patel R. Synergistic antioxidant activity of green tea with some herbs. J Adv Pharm Technol Res. 2011; 2(3): 177-183.
118. Szeto Y-T, Lei P-L ,Ngai KL , Yiu AT-W, Chan CS-P, Kok EW-F, Leong C-W. An in vitro study of the antioxidant activities and effect on human DNA of the Chinese herbal decoction ‘Liu Wei Di Huang. Int J Food Sci Nutr 2009; (60)8:662-667.
119. Sethiya NK, Maruga Raja MKM, Mishra SH. Antioxidant markers based TLC-DPPH differentiation on four commercialized botanical sources of Shankhpushpi (A Medhya Rasayana): A preliminary assessment. J Adv Pharm Technol Res 2013; 4(1):25-30.
120. Babu PS, Ignacimuthu S. Antihyperlipidemic and antioxidant effect of hyponided in the brain of streptozotocin induced diabetic rates. Int J Bio Chem 2007; (4):196-204.
121. Gupta P, Saraogi R, Khare M. Free redicals scavenging activity of polyherbal formulation (diasweta), Int J Phytopharmacol 2013; 4 (1): 24-28.
122. Jain Neha, Mishra RN. Antioxidant activity of Trikatu megaExt. Int J Res Pharma BiomedSci 2011; 2 (2):624-628.
123. Jagetia GC, Rao SK, Baliga MS, Babu KS. The Evaluation of Nitric Oxide Scavenging Activity of Certain Herbal Formulations in vitro: A Preliminary Study. Phytother Res 2004; 18:561-565.
124. Balaraman R, Bafna PA, Kolhapure, SA. Antioxidant activity of DHC-1- a herbal formulation. J Ethnopharmacol 2004; 94: 135-141.
125. Sati B, Sati H, BhattPC. Formulation and Anti Oxidant Evaluation of Ayurvedic Poly Herbal Preparation (Avaleha). Int. J. Pharm. Sci. Rev. Res., 2013; 20(1):43-46.
126. Cecchini S, Paciolla M, Caputo AR, Bavoso A Antioxidant Potential of the Polyherbal Formulation ImmuPlus A Nutritional Supplement for Horses Veterinary Medicine International 2014.
127. Makhija IK, Ram HNA, Shreedhara CS, Vijay Kumar S, Devkar R. In vitro antioxidant studies of Sitopaladi Churna, a polyherbal Ayurvedic formulation. Free Radicals and Antioxidants 2011; 1(2):37-41.
128. Shirwaikar A, Ram HNA, Mohapatra P. Antioxidant and antiulcer activity of aqueous extract of a polyherbal formulation, Indian J Exp Biol 2006; 44:474-480.
129. Shah VN, Shah MB, Bhatt PA. In vivo and In vitro Antioxidant and Hepatoprotective effects of Classical ayurvedic formulation Punarnavashtak kwath against Ethanol induced hepatotoxicity. Pharma J 2010; 2(16):43-52.
130. Kumar A, Kulkarni SK. Effect of BR-16A (Mentat®), a polyherbal formulation on drug-induced catalepsy in mice. Indian J Exp Biol 2006; 44:45-48
131. Qin F, Wu XA, Tang Y, Huang Q, Zhang ZJ, Yuan JH. Meta-analysis of randomized controlled trials to assess the effectiveness and safety of free and easy Wanderer plus, a polyherbal preparation for depressive disorders. J Psychiatr Res 2011; 45(11):1518-1524.
132. Vadthy J, Satyavati D, Dasari R,Pradeep Kumar C, Sruthi T. Evaluation of antidepressant activity of smrithi: a polyherbal formulation, Indo American J Pharm Res 2013;3(6):4471-4476.
133. Ramanathan M, Balaji B, Justin A. Behavioural and neurochemical evaluation of Perment an herbal formulation in chronic unpredictable mild stress induced depressive model. Indian J Exp Biol 2011; 49(4):269-75.
134. Bhattacharya SK1, Bhattacharya A, Chakrabarti A. Adaptogenic activity of Siotone, a polyherbal formulation of Ayurvedic rasayanas. Indian J Exp Biol. 2000; 38(2):119-28.
135. Antunes E, Gordo WM, de Oliveira JF, Teixeira CE, Hyslop S, De Nucci G.The relaxation of isolated rabbit corpus cavernosum by the herbal medicine Catuama and its constituents. Phytother Res 2001; 15(5):416-21.
136. Ma Z, Ji W, Qu R, Wang M, Yang W, Zhan Z, Fu Q, Ma A. Metabonomic Study on the Antidepressant-Like Effects of Banxia Houpu Decoction and Its Action Mechanism. Evidence-Based Complementary and Alternative Medicine 2013.
137. Hidaka T, Yonezawa R, Saito S. Kami-shoyo-san, Kampo (Japanese traditional medicine), is effective for climacteric syndrome, especially in hormone-replacement-therapy-resistant patients who strongly complain of psychological symptoms. J Obstet Gynaecol Res 2013; 39(1):223-8.
138. Afsal M, Karale S, Kamath JV. Evaluation of anticonvulsant and antidepressant activity of EPIC-Q tab: an ayurvedic polyherbal formulation, journal of pharmaceutical and scientific innovation 2014; 3(4):397-400.
139. NizamudeenT, Ramanjaneyulu J, Veeresh Babu D, Narayana Swamy VB. A Study on Antidepressant Activity of Medha gulika-a Polyherbal Formulation in Experimental Animal Models, Asian J Pharm Tech 2015;5(2):115-12.
140. Shreevathsa M, Ravishankar B, Dwivedi R. Anti depressant activity of Mamsyadi Kwatha: An Ayurvedic compound formulation. Ayu 2013; 34(1):113-117.
141. Anju D, Iswar H, Jaikumar S. Antidepressant activity of a polyherbal formulation R013, in experimental animal model. Int J Phytopharmacol 2014; 5(4):267-271.
142. Achliya GS, Wadodkar SG, Dorle AK. Evaluation of CNS activity of Bramhi Ghrita. Indian J Pharmacol 2005; 37(1):33-36.
143. Devaraj VC, Krishna BG, Viswanatha GL, Kamath JV, Kumar S. Hepatopro¬tective activity of Hepax-A polyherbal formulation. Asian Pac J Trop Biomed 2011; 1(2):142–6.
144. Mistry S, Dutt KR, Jena J. Formulation and evaluation of hepatoprotective poly¬herbal formulation containing indigenous medicinal plants. Int J Pharmtech Res2012;4(3):1238–41
145. Dandagi PM, Patil M B, Mastiholimath VS, Gadad AP, Dhumansure RH. Development and evaluation of hepatoprotective polyherbal formulation containing some indigenous medicinal plants. Indian J Pharm Sci 2008; 70(2):265-268.
146. Mayuren C, Reddy VV, Priya SV, Devi VA. Protective effect of livactine against ccl4 and paracetamol induced hepato-toxicity in adult Wister rats. N Am J Med Sci 2010 Oct; 2(10):491-495.
147. Kapur V, Pillai KK, Hussian SZ, Balani DK. Hepatoprotective activity of “jigrine" on liver damage caused by alcohol- carbontetrachloride and paracetamol in rats. Indian J Pharmacol 1994; 26:35-40.
148. Tatiya AU, Surana SJ, Sutar MP, Gamit NH. Hepatoprotective effect of poly herbal formulation against various hepatotoxic agents in rats. Pharmacognosy Res. 2012 Jan-Mar; 4(1):50-56.
149. Bhattacharyya D , Pandit S, Mukherjee R, Das N, Sur TK. hepatoprotective effect of himoliv, a polyherbal formulation in rats. IndianJ Physiol Pharmacol 2003; 47 (4):435-440
150. Venkatesh B, Uplanchiwar V, Modi, Gahane A , Jain SK , Tiwari S. Hepatoprotective effect of L-35-A polyherbal formulation, Pharmacologyonline 2010; 3:431-438.
151. Mitra SK, Seshadri SJ, Venkataranganna M V, Gopumadhavan S, Udupa UV, Sarma DNK. Effect of Hd-03-an herbal formulation in galactosamine induced hepatopathy in rats. Indian J Physiol Pharmacol 2000; 44 (1):82-86
152. Bafna PA, Balaraman R. Protective effect of DHC-1, a polyherbal formulation, against ccl4 induced liver damage. HygeiaJ D Med, 2013; 5 (1);10-18.
153. KumarEP, Vijay Rajan R , Anil Kumar D , Parasuraman S, Emerson SF. Hepatoprotective activity of Clearliv a polyherbal formulation in wistar rats. Archives of Medicine and Health Sciences 2013; 1(2):120-125.
154. Achliya GS, Wadodkar SG, Dorle AK. Evaluation of hepatoprotective effect of amalkadi ghrita against carbon tetrachloride-induced hepatic damage in rats. J Ethnopharmacol 2004; 90(2-3):229-232
155. Venkateswaran S, Pari L, Viswanathan P, Menon VP. Protective effect of Livex, a harbal formulation against erythromycin estolate induced hepatotoxicity in rats. J Ethnopharmacol 1997; 57(3):161-7.
156. Rajesh MG, Latha MS. Preliminary evaluation of the antihepatotoxic activity of Kamilari, a polyherbal formulation. J Ethnopharmacol 2004; 91(1):99-104.
157. Shah VN, Shah MB, Bhatt PA. Hepatoprotective activity of punarnavashtak kwath, an Ayurvedic formulation, against CCl4-induced hepatotoxicity in rats and on the HepG2 cell line. Pharm Biol 2011;49(4):408-15
158. Singh DP, Mani D. Protective effect of Triphala Rasayana against paracetamol-induced hepato–renal toxicity in mice. J Ayurveda Integr Med. 2015 Jul-Sep; 6(3):181–186.
159. Girish C, Koner BC, Jayanthi S, Rao KR, Rajesh B, Pradhan SC. Hepatoprotective activity of six polyherbal formulations in paracetamol induced liver toxicity in mice. Indian J Med Res 2009;129(5):569-78
160. Arsul VA, Wagh SR, Mayee RV. Hepatoprotective activity of Livergen, a polyherbal formulation against carbon tetrachloride induced hepatotoxicity in rats. Int J Pharm Pharma Sci 2011; 3(3):228-231.
161. Patra HS; Pradhan NR; Basak DK Haemato-pathological studies on the efficacy of livol in carbon tetrachloride induced hepatopathy in goats Indian J Indig Med. 1992; 8(2):17-27
162. Kenjale R, Shaikh MF, Sathaye S. Evaluation of hepatoprotective effect of polyherbal formulation – Livomyn. , Int J Res Pharm Sci 2011; 2(3):338-343.
163. Shah JS, Patel JR. Anti-ulcer activity of Lucer against experimentally induced gastric ulcers in rats. Ayu 2012; 33(2):314-316.
164. Bhujbal MM, Patoladi Quath in the management of skin disorder. Deerghayu 1999; 58:72-76,
165. Gyawali S, Khan GM, Lamichane S, Gautam J, Ghimire S, Adhikari R, Lamsal R. Evaluation of anti-secretory and anti-ulcerogenic activities ofavipattikar churna on the peptic ulcers in experimental rats. J Clin Diagn Res 2013; 7(6): 1135-1139.
166. Srinivasan D, Ramaswamy S, Sengottuvelu S. Prokinetic effect of polyherbal formulation on gastrointestinal tract. Pharmacognosy Magazine 2009; 5(17):37-42.
167. Vimal SK, Sharma D, Bhatnagar M. Anti-bacterial activity of herbal extracts, EuMiL® and antibiotics against Helicobacter muridarum. Phcog J 2010; 2(11):436-441.
168. Viswanathan V, Kesavan R, Kavitha KV, Kumpatla S. A pilot study on the effects of a polyherbal formulation cream on diabetic foot ulcers. Indian J Med Res 2011; 134(2):168–173.
169. Inoue H, Yamazaki S, Shimizu M, Uozaki H, Goto T, Ohnishi S, Koike K. Liver Injury Induced by the Japanese Herbal Drug Kamishoyosan. Gastroenterol Hepatol (N Y). 2011; 7(10):692–695.
170. Vanisree AJ, Mitra K, Shyamala DAS. Antiulcerogenic effect of UL-409 against experimentally induced gastric ulcer in rats. Indian J Pharmacol 1996; 28:265-268.
171. Dhuley JN. Words protective effect of rhinax, an herbal formulation against physical and chemical factors induced gastric and duodenal ulcers in rats. Indian J Pharmacol 1999; 31(2): 128-132.
172. Annamalai AR, Mananvalan R. Effects of Trikatu and its individual components and piperine on gastrointestinal tracts: Trikatu a bioavailable enchancer, Indian Drugs1990; 27 (12):595-604.
173. Suchalatha, S, Thirugnanasambandam, P, Maheswaran, E, Shyamala DSS. Role of Arogh, a polyherbal formulation to mitigate oxidative stress in experimental myocardial infarction. Indian J Exp Biol 2004; 42:224-226.
174. Upaganlawar A, Gandhi H, B alaraman R. Isoproterenol induced myocardial infarction: protective role of natural products, J Pharmacol Toxicol 2011; 6 (1):1-17.
175. Oh J, Lee H, Lim H, Woo S, Shin SS, Yoon M. The herbal composition GGEx18 from Laminaria japonica, Rheum palmatum, and Ephedra sinica inhibits visceral obesity and insulin resistance by upregulating visceral adipose genes involved in fatty acid oxidation. Pharm Biol 2015; 53 (2):301-312,
176. Kamali SH, Khalaj AR, Hasani-Ranjbar S, Esfehani MM, Kamalinejad M, Soheil O, Kamali SA. Efficacy of ‘Itrifal Saghir’, a combination of three medicinal plants in the treatment of obesity; A randomized controlled trial. Daru 2012; 20(1):33.
177. Singh K, Patki PS, Mitra SK. Evaluation of clinical efficacy of ayurslim on body weight, body mass index, lipid profile and skin fold thickness: a phase iv clinical study. The Antiseptic2005; 105 (5):241-243.
178. Ignjatovic V, Ogru E, Heffernan M, Libinaki R, Lim Y, Ng F. Studies on the Use of ‘Slimax’, a Chinese Herbal Mixture, in the Treatment of Human Obesity. Pharm Biol 2000; 38(1);30-35.
179. Toromanyan E, Aslanyan G, Amroyan E, Gabrielyan E, Panossian A. Efficacy of Slim339 in reducing body weight of overweight and obese human subjects. Phytotherapy Research 2007; 21(12):1177-1181.
180. Kaur G, Kulkarni SK. Differential effect of polyherbal, antiobesity preparation, OB-200G in male and female mice and monosodium –glutamate related rates. Indian J Pharmacol Exp Biol 2001; 39:551-557.
181. Sumanth M, Swetha S. Anti hypercholesterolaemic activity of Lipovedic and its mechanism. Int J Green Pharm2013; 155-161.
182. Mishra LC. scientific Basis for Ayurvedic Therapies, CRC PRESS, London, New York, Washington, D.C 2004, 161-163.
183. Sarker MR, Imam H, Bhuiyan MS, Choudhuri KMS. In vitro assessment of prasarani sandhan, a traditional polyherbal ayurvedic medicine, for immunostimulating activity in splenic cells of balb/c mice. Int J Pharm Pharm Sci 2014; 6(9):531-534.
184. Sabina EP, Rasool MK, Mathew L. In vivo and in vitro immunomodulatory effects of indian ayurvedic herbal formulation triphala on experimental induced inflammation, Pharmacologyonline 2009; 2:840-849.
185. Chatterjee S Das SN. Immunopotentiation by Immu21 in immunocompromised rat. Ancient science of Life 1997; XVI 3:200-205.
186. Sharma SB, Ray S. Effect of herbal preparation on immune response of immunosuppressed mice. Indian J Physiol Pharmacol 1997; 41(3):293-6.
187. Kshirsagar M, Palaniyamma D, Gopumadhavan S, Patki PS. Clinical Efficacy and Safety of Septilin Tablets in Respiratory Tract Infections: A Meta-analysis. Indian J Clin Pract 2010; 20 (8): 595-600.
188. Kulkarni SK. BR-16A (Mentat) - Multicomponent Herbal Psychotropic Formulation. Drugs of the Future, 1996; 21(6):585-
189. Preeti T, Patil PA, Majagi SI. Effect of mentat and its selected ingredients on 3-nitropropionic acid induced neuronal damage in wistar rats. Pharmacologyonline2010; 1:856-863
190. Zee-Cheng RK. Shi-quan-da-bu-tang (ten significant tonic decoctions), SQT. A potent Chinese biological response modifier in cancer immunotherapy, potentiation and detoxification of anticancer drugs. Methods Find Exp Clin Pharmacol 1992; 14(9):725-36.
191. Sarker MR, Nahar S, Shahriar M, Seraj S, Choudhuri SK. Preliminary study of the immunostimulating activity of an ayurvedic preparation, Kanakasava, on the splenic cells of BALB/c mice in vitro. Pharm Biol 2012; 50(11): 1467–1472.
192. Ilodigwe EE, Ndunagu LU, Ajaghaku DL, Utoh-Nedosa UA. Evaluation of the wound healing activity of a polyherbal remedy. Annals Bioll Res 2012; 3 (11):5393-5398.
193. Fahimi S, Abdollahi M, Mortazavi SA, Hajimehdipoor H, Abdolghaffari AH, Rezvanfar MA. Wound healing activity of a traditionally used poly herbal product in a burn wound model in rats. Iran Red Crescent Med J 2015; 17(9):e19960.
194. Kumar MS, Kirubanandan S, Sripriya R, Sehgal PK. Triphala promotes healing of infected full-thickness dermal wound. J Surg Res 2008; 144(1):94-101.
195. Khalil EA, Afifi FU, Al-Hussaini M. Evaluation of the wound healing effect of some jordanian traditional medicinal plants formulated in Pluronic F127 using mice (mus musculus). J Ethnopharmacol 2007; 109:104-112.
196. Krishna M, Vijay L, Mayank P, Shah M, Gajera V. Evaluation of wound healing activity of polyherbal formulation in rats. Res J Pharmacog Phytochem 2011; 3 (3):112-115.
197. Pawar PL, Nabar BM. effect of plant extracts formulated in different ointment bases on mdr strains. Indian J Pharm Sci 2010; 72(3): 397-401.
198. Nema A, Pathak AK, Choubey A, Singh R, Shivhare Y. Evaluation of a polyherbal preparation for wound healing activity. Res J Pharmacol Pharmacody 2010; 2(5), 340-342.
199. Sathish HS, Jyothi T, Ashok BK, Vaghela DB, Bhuyan C, Ravishankar B. Wound healing activity of Jati Kalpa Ghrita in albino rats. Ayurpharm Int J Ayur Alli Sci 2013; 2 (8): 242-247.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeÂ The Effect of Open Access).