In-vitro studies on inhibition of alpha amylase and alpha glucosidase by plant extracts of alternanthera Pungens kunth
The aim of this work was to evaluate the alpha amylase and alpha glucosidase inhibitory activities of ethanolic and aqueous extract of whole plant of Alternanthera Pungens Kunth. Diabetes mellitus is a group of metabolic disorder characterized by hyperglycemia this may be due to defects in insulin secretion, insulin action or both. Alpha amylase and alpha glucosidase inhibitors are used to control hyperglycemia in type 2 diabetes mellitus. The dried whole plant was subjected for extraction with ethanol (95% v/v) by continuous hot soxhletion apparatus and cold maceration with distilled water (0.25 % chloroform). The ethanolic and aqueous extracts of plant were used to study alpha amylase and alpha glucosidase activity, where Acarbose was used as a positive control. A significant effect of ethanolic and aqueous extract in alpha amylase and alpha glucosidase were observed. The alpha amylase inhibition activity of ethanolic and aqueous extracts of Plant showed IC50 value of 6.96±1.47 and 7.54±0.73 µg/mL respectively when compared with acarbose (IC50 value 7.77±1.42) and in alpha glucosidase assay, the inhibition activity of plant extracts have IC50 values 76.78±1.76 and 70.62±1.28_µg/mL respectively when compared to acarbose (IC50 value 81.85±1.46). As the extracts contains flavonoids which could be good candidate for inhibition of alpha amylase and alpha glucosidase enzymes. The present study supports the traditional use of this plant in treatment of Diabetes.
Keywords: Diabetes mellitus, Alternanthera Pungens Kunth, Alpha-Amylase, Alpha Glucosidase.
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