In-vitro anti-inflammatory activity of different extracts of flowers of Tridex procumbens
AIM- The main of study is to evaluate the In-vitro anti-inflammatory activity of different extracts of flowers of Tridex procumbens. Material & Methods-Successive solvent extraction method was followed for extraction by using different solvents i.e. petroleum ether, chloroform, methanol, butanol and water. Preliminary phytochemical screening method was performed for the presence of different phytoconstituents. For the evaluation of anti-inflammatory activity, protein denaturation and proteinase inhibitory method were performed. Diclofenac sodium was used as standard. Result- Among all the extracts, methanolic extract of Tridex procumbens showed 80.23% inhibition. The petroleum ether, chloroform, n-butanolic and water extract had shown the 12.23, 23.33, 20.45 and 36.20% inhibition respectively at 500 µg/ml concentration. The Diclofenac sodium showed 83.28 % inhibition against denaturation of protein. The different extracts were evaluated by using this method. Methanolic extract of Tridex procumbens showed 74.50% inhibition at 500 µg/ml. The petroleum ether, chloroform, n-butanolic and water extracts had shown the 30.16%, 41.22%, 43.28% and 31.97% inhibition respectively at 500 µg/ml. The Diclofenac sodium showed 79.21% inhibition against proteinase inhibitory activity at 100 µg/ml. Conclusion- Besides from the obvious therapeutic importance, methanolic extract would be useful in understanding the mechanism of diseases with higher levels of cellular and molecular level. These components could serve as lead molecules for development of prospective anti-arthritic agents. Further detailed studies are required to isolate the active phytoconstituents from methanolic extract which is responsible for anti-arthritic activity.
Keywords: Protein denaturation model, proteinase inhibitory method, methanolic extract, anti-inflammatory activity, Tridex procumbens
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