In-vitro anti-inflammatory activity of different extracts of flowers of Tridex procumbens

  • Sanjay Giri Department of Pharmacology, Oriental University, Indore (M.P.)-India
  • Neetesh K Jain Department of Pharmacology, Oriental University, Indore (M.P.)-India
  • Anis Shaikh Institute of Pharmacy, Vikram University, Ujjain (M.P.)-India

Abstract

AIM- The main of study is to evaluate the In-vitro anti-inflammatory activity of different extracts of flowers of Tridex procumbens. Material & Methods-Successive solvent extraction method was followed for extraction by using different solvents i.e. petroleum ether, chloroform, methanol, butanol and water. Preliminary phytochemical screening method was performed for the presence of different phytoconstituents. For the evaluation of anti-inflammatory activity, protein denaturation and proteinase inhibitory method were performed. Diclofenac sodium was used as standard. Result- Among all the extracts, methanolic extract of Tridex procumbens showed 80.23% inhibition. The petroleum ether, chloroform, n-butanolic and water extract had shown the 12.23, 23.33, 20.45 and 36.20% inhibition respectively at 500 µg/ml concentration. The Diclofenac sodium showed 83.28 % inhibition against denaturation of protein. The different extracts were evaluated by using this method. Methanolic extract of Tridex procumbens showed 74.50% inhibition at 500 µg/ml. The petroleum ether, chloroform, n-butanolic and water extracts had shown the 30.16%, 41.22%, 43.28% and 31.97% inhibition respectively at 500 µg/ml. The Diclofenac sodium showed 79.21% inhibition against proteinase inhibitory activity at 100 µg/ml. Conclusion- Besides from the obvious therapeutic importance, methanolic extract would be useful in understanding the mechanism of diseases with higher levels of cellular and molecular level. These components could serve as lead molecules for development of prospective anti-arthritic agents. Further detailed studies are required to isolate the active phytoconstituents from methanolic extract which is responsible for anti-arthritic activity.


Keywords: Protein denaturation model, proteinase inhibitory method, methanolic extract, anti-inflammatory activity, Tridex procumbens

Downloads

Download data is not yet available.

Author Biographies

Sanjay Giri, Department of Pharmacology, Oriental University, Indore (M.P.)-India

Department of Pharmacology, Oriental University, Indore (M.P.)-India

Neetesh K Jain, Department of Pharmacology, Oriental University, Indore (M.P.)-India

Department of Pharmacology, Oriental University, Indore (M.P.)-India

Anis Shaikh, Institute of Pharmacy, Vikram University, Ujjain (M.P.)-India

Institute of Pharmacy, Vikram University, Ujjain (M.P.)-India

Crossmark
Statistics
457 Views | 266 Downloads
How to Cite
1.
Giri S, Jain NK, Shaikh A. In-vitro anti-inflammatory activity of different extracts of flowers of Tridex procumbens. JDDT [Internet]. 10Jan.2019 [cited 18Apr.2024];8(6-A):111-5. Available from: https://jddtonline.info/index.php/jddt/article/view/2298
Issue
Vol 8 No 6-A (2018): VOLUME 8, ISSUE 6-A 2018
Section
Research
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).