Immunomodulatory Activity of Different Extracts of Stem Bark of Alstonia scholaris Linn
Aim- The main of the study is to evaluate the immunomodulatory activity of different extracts of stem bark of Alstonia scholaris. Material & Methods- Different extracts were prepared by successive solvent extraction methods according to polarity of solvents. Preliminary phytochemical screenings were performed for the presence of various active phytochemicals. For the dose determination of different extracts, an OECD guideline was used and 200, 400 mg/kg were used for activity. for immunomodulatory activity, SRBC, phagocytic index and cyclophosphamide model were used. Result- The cell-mediated immune response of different extracts of Alstonia scholaris were assessed by DTH reaction, i.e. foot pad reaction. The chloroform and methanolic extract of Alstonia scholaris produced a significant, dose-related increase in DTH reactivity in rats. Chloroform and methanolic extracts (400 mg/kg) showed highest phagocytic index 6.44±0.57 & 6.67±0.48 respectively. The phagocytic index of control (group I) was 3.78±0.22. In case of cyclophosphamide induced myelosupression, there was decrease in the WBCs count in the control group. In treatment groups, the RBCs, HB and WBC count was found to be increased with p<0 .01and p< 0.001 at 200 & 400 mg/kg dose respectively on 11th day. Conclusion-On the basis of the results obtained in the present study it can be concluded that stem bark of Alstonia scholaris had the potential to stimulate the humoral immune response and cell-mediated immune response and it may be a potential candidate in several immuno-suppressed clinical conditions.
Keywords: DTH Response, Alstonia scholaris, Stem Bark, Carbon Clearance, Phagocytic Index
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