Novel Method development and Validation of Bortezomib in Bulk and Pharmaceutical dosage form by RP- HPLC

  • Pasupuleti Laxmi Prasanna Asst. Prof, Department of pharmaceutical Analysis, Vikas college of pharmaceutical sciences, Rayanigudem, Suryapet(M), Suryapet (Dist), Telangana-508213
  • Pedapanga Sandhya Asst. Prof, Department of pharmaceutical Analysis, Vikas college of pharmaceutical sciences, Rayanigudem, Suryapet(M), Suryapet (Dist), Telangana-508213
  • Pittala Geetha Asst. Prof, Department of pharmaceutical Technology, Vikas college of pharmaceutical sciences, Rayanigudem, Suryapet(M), Suryapet (Dist), Telangana-508213
  • Vallapatla M Anuhya Asst. Prof, Department of pharmaceutical Technology, Vikas college of pharmaceutical sciences, Rayanigudem, Suryapet(M), Suryapet (Dist), Telangana-508213

Abstract

A simple, rapid, precise and accurate RP-HPLC method was developed and validated for the determination of Bortezomib, in bulk and pharmaceutical dosage form. The separation is achieved on RP-HPLC using a PDA detector by incorporation of enpower 2 software with a flow rate of 1.0ml/min using a mixture of Methanol and water (15:85% v/v) as mobile phase. The column used was Hypersil C18 (4.6×150mm, 5µ) at a wave length of 284nm. The retention time of the Bortezomib was 3.515min.. The linearity of the drug was 25-125µg/m and the method precision for the determination of assay was below 2.0% RSD. The proposed method was validated and applied for the estimation of Bortezomib in quality control of bulk and pharmaceutical dosage forms.


Keywords: Bortezomib, RP-HPLC, validation.

Downloads

Download data is not yet available.

Author Biographies

Pasupuleti Laxmi Prasanna, Asst. Prof, Department of pharmaceutical Analysis, Vikas college of pharmaceutical sciences, Rayanigudem, Suryapet(M), Suryapet (Dist), Telangana-508213

Asst. Prof, Department of pharmaceutical Analysis, Vikas college of pharmaceutical sciences, Rayanigudem, Suryapet(M), Suryapet (Dist), Telangana-508213

Pedapanga Sandhya, Asst. Prof, Department of pharmaceutical Analysis, Vikas college of pharmaceutical sciences, Rayanigudem, Suryapet(M), Suryapet (Dist), Telangana-508213

Asst. Prof, Department of pharmaceutical Analysis, Vikas college of pharmaceutical sciences, Rayanigudem, Suryapet(M), Suryapet (Dist), Telangana-508213

Pittala Geetha, Asst. Prof, Department of pharmaceutical Technology, Vikas college of pharmaceutical sciences, Rayanigudem, Suryapet(M), Suryapet (Dist), Telangana-508213

Asst. Prof, Department of pharmaceutical Technology, Vikas college of pharmaceutical sciences, Rayanigudem, Suryapet(M), Suryapet (Dist), Telangana-508213

Vallapatla M Anuhya, Asst. Prof, Department of pharmaceutical Technology, Vikas college of pharmaceutical sciences, Rayanigudem, Suryapet(M), Suryapet (Dist), Telangana-508213

Asst. Prof, Department of pharmaceutical Technology, Vikas college of pharmaceutical sciences, Rayanigudem, Suryapet(M), Suryapet (Dist), Telangana-508213

References

1. Dr. Kealey and P.J Haines, Analytical Chemistry, 1stedition, Bios Publisher, (2002), PP 1-7.
2. A.BraithWait and F.J.Smith, Chromatographic Methods, 5thedition, Kluwer Academic Publisher, (1996), PP 1-2.
3. Andrea Weston and Phyllisr. Brown, HPLC Principle and Practice, 1st edition, Academic press, (1997), PP 24-37.
4. Yuri Kazakevich and Rosario Lobrutto, HPLC for Pharmaceutical Scientists, 1stedition, Wiley Interscience A JohnWiley & Sons, Inc., Publication, (2007), PP 15-23.
5. Chromatography, (online). URL:http://en.wikipedia.org/wiki/Chromatography.
6. Meyer V.R. Practical High-Performance Liquid Chromatography, 4 Ed. England, John Wiley & Sons Ltd, (2004), PP 7-8.
7. Sahajwalla CG a new drug development, vol 141, Marcel Dekker Inc., New York, (2004), PP 421–426.
8. Introduction to Column. (Online),URL:http://amitpatel745.topcities.com/index_files/study/co lumn care.pdf
9. Detectors used in HPLC (online )URL:http://wiki.answers.com/Q/What_detectors_are_used_ in_HPLC
10. Detectors (online) ,URL:http://hplc.chem.shu.edu/NEW/HPLC_Book/Detectors/det_uvda.ht ml
11. URL:https://www.ninlaro.com/
12. URL:http://businesswireindia.com/news/news-details/us-fda-approves-takedas-ninlaro- Bortezomib-first-only-oral-proteasome-inhibitor-treat-multiple-myeloma/46254
13. URL:www.medkoo.com
14. URL:https://en.wikipedia.org/wiki/Bortezomib
Statistics
136 Views | 140 Downloads
How to Cite
Laxmi Prasanna, P., Sandhya, P., Geetha, P., & Anuhya, V. M. (2019). Novel Method development and Validation of Bortezomib in Bulk and Pharmaceutical dosage form by RP- HPLC. Journal of Drug Delivery and Therapeutics, 9(1), 17-21. https://doi.org/10.22270/jddt.v9i1.2259