In silico approach of receptor-ligand binding and interaction: Established phytoligands from Tagetes errecta Linn. against bacterial β-glucosidase receptor

  • Madhumita Lahiri Department of Botany, Serampore College, University of Calcutta, William Carey Road, Hooghly, West Bengal, India
  • Partha Talukdar Department of Botany, Serampore College, University of Calcutta, William Carey Road, Hooghly, West Bengal, India
  • Soumendra Nath Talapatra Department of Biological Science, Seacom Skills University, Kendradangal, Shantiniketan, Birbhum – 731236, West Bengal, India

Abstract

The medicinal plant, Tagetes errecta Linn. is a common ornamental plant and leaves of this plant are containing phytochemicals (volatile oil) that inhibit the growth of bacteria, fungi and known natural antimicrobial agents. The objective of the present study was to detect receptor-ligand binding energy and interaction through molecular docking for phytoligands established in the leaves of T. errecta against β-glucosidase receptor (PDB ID: 3AHZ). Molecular docking was performed by using PyRx (Version 0.8) for the structure-based virtual screening and visualized the interaction in the molecular graphic laboratory (MGL) tool (Version 1.5.6). Among 25 phytochemicals and 2 synthetic compounds (Carbendazim and 2-Amino-2-hydroxymethyl-propane-1,3-diol), binding energy value was obtained highest in Bicyclogermacrene (-6.4 Kcal/mol) and lowest in Octanol (-4.4 Kcal/mol) and Carbendazim and 2-Amino-2-hydroxymethyl-propane-1,3-diol showed -6.7 Kcal/mol and -3.5 Kcal/mol all of these showed no hydrogen bonding. The binding interaction of target protein with this phytocompound found binding at the mouth of the active site may be treated as competitive inhibitor. In conclusion, phytocompound Bicyclogermacrene can be alternative of synthetic fungicide as per binding energy value and interaction. It is suggesting further pharmacological and toxicological assay with this phytocompound after isolation from ornamental plant (T. errecta).

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Author Biographies

Madhumita Lahiri, Department of Botany, Serampore College, University of Calcutta, William Carey Road, Hooghly, West Bengal, India

Department of Botany, Serampore College, University of Calcutta, William Carey Road, Hooghly, West Bengal, India

Partha Talukdar, Department of Botany, Serampore College, University of Calcutta, William Carey Road, Hooghly, West Bengal, India

Department of Botany, Serampore College, University of Calcutta, William Carey Road, Hooghly, West Bengal, India

Soumendra Nath Talapatra, Department of Biological Science, Seacom Skills University, Kendradangal, Shantiniketan, Birbhum – 731236, West Bengal, India

Department of Biological Science, Seacom Skills University, Kendradangal, Shantiniketan, Birbhum – 731236, West Bengal, India

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How to Cite
Lahiri, M., Talukdar, P., & Talapatra, S. N. (2019). In silico approach of receptor-ligand binding and interaction: Established phytoligands from Tagetes errecta Linn. against bacterial β-glucosidase receptor. Journal of Drug Delivery and Therapeutics, 9(1-s), 125-131. https://doi.org/10.22270/jddt.v9i1-s.2246