Formulation and evaluation of chitosan films containing sparfloxacin for the treatment of periodontitis

  • Santoshi Naik Department of Pharmaceutics, Yenepoya Pharmacy College and Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore.
  • Prasiddhi Raikar Department of Product Development, Tetramed Biotek, Baner, Pune.
  • Mohammed Gulzar Ahmed Department of Pharmaceutics, Yenepoya Pharmacy College and Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore.

Abstract

In the present study an attempt has been made to formulate and evaluate a sustained release periodontal film of Sparfloxacin with biodegradable, cost effective polymer Chitosan. The objective of the study was to formulate intra-pocket periodontal films, which could be easily placed into the periodontal pocket, and thus be capable of delivering therapeutic concentrations of drug. Sparfloxacin is an antibiotic, showing wide spectrum antibacterial activity against a number of periodontal pathogens. Hence Sparfloxacin is selected as model for site specific delivery, i.e., into periodontal pocket for the treatment of periodontitis. In the present investigation Chitosan films containing Sparfloxacin were prepared by solution casting method using acetic acid. The copolymers HPMC K4M, Sodium CMC and Eudragit RL 100 in the concentrations of 10%, 20% and 30% w/w of Chitosan were added into the polymeric solution. Propylene glycol was used as plasticizer. FT-IR and UV spectroscopic methods revealed no interaction between Sparfloxacin and polymers. The drug loaded films were evaluated for their thickness, weight variation, content uniformity, tensile strength, percent elongation, percentage moisture loss, surface pH, folding endurance, in- vitro drug release studies, in - vitro antibacterial activity and stability studies. Periodontal films showed initial burst release of drug on 1st day and then the release was sustained for a period of 8 days. In – vitro antibacterial activity was carried out on staphylococcus aureus and the antibacterial activity was retained for 96 hours. In - vitro release from periodontal films was fit to kinetic models to reveal drug release kinetics.


Keywords: Periodontitis, Sparfloxacin, Bio-adhesive polymers.

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Author Biographies

Santoshi Naik, Department of Pharmaceutics, Yenepoya Pharmacy College and Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore.

Department of Pharmaceutics, Yenepoya Pharmacy College and Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore.

Prasiddhi Raikar, Department of Product Development, Tetramed Biotek, Baner, Pune.

Department of Product Development, Tetramed Biotek, Baner, Pune.

Mohammed Gulzar Ahmed, Department of Pharmaceutics, Yenepoya Pharmacy College and Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore.

Department of Pharmaceutics, Yenepoya Pharmacy College and Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore.

References

1. Sawarkar. S.P., Kadam. A. Novel Treatment Approaches for Maintenance of Dental Hygiene. Pharma Times. 2016; 48(3).
2. D. F. Kinane, and J. Lindhe. Pathogenesis of periodontitis. In J. Lindhe , T. Karring, and N. P. Lang (eds.), Clinical Periodontology and Implant Dentistry, Munksgaard, Copenhagen, 1998:189-225.
3. American Academy of Periodontology. “Proceedings of International Workshop for the classification of periodontal diseases”, Ann. Periodontal., in press. 2000.
4. Schwach-Abdellaouia K, Vivien-Castionib N, Gurny R. Local delivery of antimicrobials for the treatment of periodontal diseases. European Journal of Pharmaceutics and Biopharmaceutics. 2000; 50:83-99.
5. Southard GL, Godowski KC. Subgingival controlled release of antimicrobial agents in the treatment of periodontal disease. International Journal of Antimicrobial Agents. 1998; 9:239-253.
6. Seymour R.A, Heasmen PA. Pharmacological control of periodontal disease. II. Antimicrobial agents. Journal of Dentistry. 1995; 23(1):5-14.
7. Mayanagi G, Sato T, Shimauchi H, Takahashi N. Microflora profiling of subgingival and supragingival plaque of healthy and periodontitis subjects by nested PCR. International Congress Series. 2005; 1284:195-196.
8. Wanga PL, Shirasub S, Shinoharaa M, Daitob M, Oidoc M, Kowashic Y, Ohuraa K. Induction of apoptosis in human gingival fibroblasts by a Porphyromonas gingivalis protease preparation. Archives of Oral Biology. 1999; 44:337-342.
9. V. Parthasarathy,R. Manavalan, R. Mythili, Chennankara T. Siby, and M. Jeya Ethyl Cellulose and Polyethylene Glycol-Based Sustained-Release Sparfloxacin Chip: An Alternative Therapy for Advanced Periodontitis. Drug Development and Industrial Pharmacy. 2002; 28(7):849–862.
10. Borude A. D, Mahale N. B. Formulation and Evaluation of dental implant of Moxifloxacin HCl for the treatment of Periodontitis. International Journal of Pharmacy and Biological Science. 2013; 3(4):49-55.
11. S. Rani, N.Singh. Formulation and characterization of Periodontal films containing Azithromycin and Serratiopeptidase. Asian Journal of Pharmaceutical and Clinical Research. 2018; 11(5): 205-209.
12. Katiyar Aviral, Prajapati S. K., Akhtar Ali, Vishwakarma Sanjay Kumar. Formulation and Evaluation of dental film for Periodontitis. International Research Journal of Pharmacy. 2012; 3(10):143-148.
13. Ashwin Kumar J, Saarangi Ramesh, Ramesh. J, B. Sudhakar, P. Goverdhan Reddy. Design and Evaluation of biodegradable Periodontal films containing Ciprofloxacin and Ornidazole. Scholars Academic Journal of Pharmacy. 2013; 2(2):60-69.
14. Fulzele SV, Sattuwar PM, Dorle AK. Polymerized rosin: Novel film forming polymer for drug delivery. Int J Pharm 2002;249:175-84.
15. Nakahara T, Nakamura T, Kobayashi E, Inoue M, Shigeno K. Novel approach to regeneration of periodontal tissues based on in situ tissue engineering: Effects of controlled release of basic fibroblast growth factor from a sandwich membrane. Tissue Eng 2003;9:153-62.
16. N. Deepthi, G. Velrajan. Formulation and Evaluation of Moxifloxacin Periodontal films. International Journal of Pharma and Bio Sciences. 2013; 4(2):549-555.
17. Brahmankar DM, Jaiswal SB, Biopharmaceutics and pharmacokinetics, A treatise.1st edition. 1995:53-61.
18. Suvakanta Dash, Padala Narasimha Murthy, Lilakanta Nath, Prasanta Chowdhury. Kinetic Modelling on drug release from controlled Drug delivery systems. Acta Poloniae Pharmaceutica and Drug Research. 2010; 67(3):217-223.
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Naik, S., Raikar, P., & Ahmed, M. G. (2019). Formulation and evaluation of chitosan films containing sparfloxacin for the treatment of periodontitis. Journal of Drug Delivery and Therapeutics, 9(1), 38-45. https://doi.org/10.22270/jddt.v9i1.2245