Physicochemical, Qualitative and Quantitative Determination of Secondary Metabolites and Antioxidant Potential of Kalanchoe Pinnata (Lam.) Pers. Leaf Extracts

  • Santosh Kumar Singh Ph.D. Scholar, Pacific college of Pharmacy, Pacific University, Pacific Hills, Pratap Nagar Extension, Debari, Udaipur-313024, Rajasthan, India
  • Jay Ram Patel Principal, Rewa College of Pharmacy, NH-7, Allahabad Road, Ratahra, Rewa- 486001, Madhya Pradesh, India
  • Arvind Dangi Truba Institute of Pharmacy, Karond, Bhopal-462038, Madhya Pradesh, India.

Abstract

Kalanchoe pinnata (Lam.) Pers. has many pharmacological properties such as anticancer, antidiabetic, insecticidal, antimicrobial, anti-urolithiatic, etc. The plant leaves are used as ethno medicine traditionally. Many diseases are associated with oxidative stress caused by free radicals. The aim of the present study was to evaluate physicochemical, qualitative and quantitative phytochemical analysis and in vitro antioxidant activities of leaf of Kalanchoe pinnata collected from Bhopal region of Madhya Pradesh.The physicochemical evaluations carried out in terms of loss on drying, ash value, extractive values and acid insoluble ash value ect. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by FolinsCiocalteau reagent method and Aluminium chloride method respectively. The In vitro antioxidant activity of ethanolic extract of the leaf was assessed against DPPH assay method using standard protocols. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids. The total phenolics content of leaves ethanolic extract was (4.63mg/100mg), followed by flavonoids (2.03mg/100mg). The activities of ethanolic leaves extract against DPPH assay method were concentration dependent with IC 50 values of ascorbic acid and extracts 24.44 and 49.81μg/ml respectively. These studies provided information for standardization and correct identification of this plant material. The diverse array of phytochemicals present in the plant thus suggests its therapeutic potentials which may be explored in drug manufacturing industry as well as in traditional medicine.


Keywords: Kalanchoe pinnata, Physicochemical, Qualitative, Quantitative phytochemical, Antioxidant

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Author Biographies

Santosh Kumar Singh, Ph.D. Scholar, Pacific college of Pharmacy, Pacific University, Pacific Hills, Pratap Nagar Extension, Debari, Udaipur-313024, Rajasthan, India

Ph.D. Scholar, Pacific college of Pharmacy, Pacific University, Pacific Hills, Pratap Nagar Extension, Debari, Udaipur-313024, Rajasthan, India

Jay Ram Patel, Principal, Rewa College of Pharmacy, NH-7, Allahabad Road, Ratahra, Rewa- 486001, Madhya Pradesh, India

Principal, Rewa College of Pharmacy, NH-7, Allahabad Road, Ratahra, Rewa- 486001, Madhya Pradesh, India

Arvind Dangi, Truba Institute of Pharmacy, Karond, Bhopal-462038, Madhya Pradesh, India.

Truba Institute of Pharmacy, Karond, Bhopal-462038, Madhya Pradesh, India.

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How to Cite
Singh, S. K., Patel, J. R., & Dangi, A. (2019). Physicochemical, Qualitative and Quantitative Determination of Secondary Metabolites and Antioxidant Potential of Kalanchoe Pinnata (Lam.) Pers. Leaf Extracts. Journal of Drug Delivery and Therapeutics, 9(1), 220-224. https://doi.org/10.22270/jddt.v9i1.2225