EFFECT OF FLAVANOID RICH FRACTION OF CITRUS MEDICA LINN. (RUTACEA) ON ETHYLENE GLYCOL INDUCED UROLITHIASIS IN RATS.
Ethylene glycol (0.75% v/v p.o. in drinking water; 28 days) induced urolithiasis was used to study the protective effect of Â flavanoid rich fraction of Citrus medica Linn. (FFCM)Â at three dose level (320 Âµg/kg; 380 Âµg/kg; 440 Âµg/kg-Â 28 days; p.o.) in male wistar albino rats (250-300g; n=6/group).Â Cystone (750 mg/kg; p.o.) was used as standard drug. After completion of treatment period of 28 days, Â 24 hr urine sample and blood were collected. Various physical parameters like body weight, diuresis, pH, kidneys weight (wet and dry) were measured. Various stone forming inhibitors (Magnesium and Citrate) and promoters (Oxalate, Calcium, Phosphate, Uric acid and Urea) Â were analysed in urine, serum and kidney homogenate. Renal function test (BUN and Creatinine clearance), antioxidant parameters (MDA and Catalase) and crystalluria were also evaluated. FFCM at all dose level significantly prevented EG induced changes in calcium, inorganic phosphate, uric acid, oxalate, urea, citrate, magnesium level; creatinine clearance and oxidative stress. FFCM possess anti-lithiatic activity in experimentally induced urolithiatic model (Ethylene glycol model), that can be attributed to its diuretic action, decrease in promoters and increase in inhibitors level & antioxidant potential.
Key words: Urolithiasis, Citrus medica Linn., Calcium oxalate, Ethylene glycol.
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