In vitro study on screening antimicrobial and anti-oxidant potential of Ramalina fastigiata
Abstract
The aim of the present study is to investigate the antimicrobial and anti-oxidant potential of lichen Ramalina fastigiata collected from Kolli hills, Eastern Gahts of Tamil Nadu, India. Phytochemical study revealed that acetone extract of Ramalina fastigiata confirmed the presence of flavonoids, glycosides and phenols. Acetone extract of Ramalina fastigiata was tested against human pathogens, which exposed antimicrobial activity against Klebsiella pneumonia and Candida krusei with the inhibition rate of 2.1 mm and 1.3 mm respectively. The acetone extract of lichen Ramalina fastigiata exhibited significant antioxidant activity as well. Radical scavenging ability of Ramalina fastigiata was reported in terms of 61.53 % inhibition.
Keywords: Lichen, Ramalina fastigiata, acetone extract, antimicrobial activity, anti-inflammatory potential
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References
1. Ananthi R, Tinabaye A, Selvaraj G, Antioxidant study of usnic acid and its derivative usnic acid diacetate”, International Journal of Research in Engineering and Technology,2015;4:356-366.
2. Awasthi DD, The typification of Roccella montagnei, The Bryologist, 1981; 84(2):216--219.
3. Bauer AW, Kirby WMM., Sherris JC, Turck M, Antibiotic susceptibility testing by a standardized single disc method, American Journal of Clinical Pathology, 1966; 45(4):493.
4. Karthikaidevi G, Thirumaran G, Manivannan K, Anantharaman P, Kathiresan K, Balasubaramanian T, Antimicrobial activities of the lichen Roccella belangeriana (awasthi) from mangroves of mannar, Indian journal of geo-marine sciences, Vol. 2011; 40(3):449-453.
5. Rashmi S, Rajkumar HG, Preliminary phytochemical screening of different solvent extracts of lichens from kodagu district, Karnataka, Journal of pharmacognosy and phytochemistry, 2014; 3(4):209-212.
6. Revathy M, Sathya SS, Manimekala N, Annadurai G, Ahila M, Preliminary phytochemical investigation and antibacterial effects of lichen Parmotrema perlatum against human pathogens, European journal of biomedical and pharmaceutical sciences, 2015; 2:336-347.
7. Nayaka S, Methods and techniques in collection, preservation and identification of lichens. In plant Taxonomy and Biosystematics–Classical and Modern Methods (Eds T.S. Rana, K.N. Nair and D.K. Upreti). New India Publishing Agency, New Delhi 2014; 101-128.
8. Saliha S, Seyhan O, Pinar S, Cevdet D, Sule O, Ramalina lichens and their major metabolites as possible natural antioxidant and antimicrobial agents, Journal of Food Biochemistry, 2015; 3(9):471-477.
9. Poornima S, Ponmurugan P, Mythili Gnanamangai B, Nagarjun N, Ayyappadasan G. Evaluation of antimicrobial, antioxidant and anticancer activities of few macrolichens collected from Eastern Ghats of Tamil Nadu, India, International Research Journal of Pharmacy, 2017; 8(3):39-43.
10. Behra BC, Verma N, Sonone A, Makhjia U, Evaluation of antioxidant potential of the cultured mycobionts of a lichen Usnea ghattensis, Phytotherapy Research 2005; 19: 58-64.
11. Subhash Gaikwad, Neeraj Verma, Sharma BO, Behra BC, Growth promoting effects of some lichen metabolites on probiotic bacteria, Journal of Food Science and Technology 2012; 51(10):2624-2631.
12. Sharma BC, Kalikoty S, Screening of antioxidant activity of lichens Parmotrema reticulatum and Usnea sp. from Darjeeling hills, India, IOSR Journal of Pharmacy, 2012; 2(6): 54-60.
13. Choi Y, Jeong H, Lee J, Antioxidant activity of methanolic extracts from some grains consumed in Korea, Food Chemistry, 2007; 103:130-138.
14. Jayaprakasha GK, Rao LJ. Phenolic constituents from the lichen Parmotrema stuppeum (Nyl.) Hale and their antioxidant activity, A Journal of Biosciences, 2000; 55: 1018-1022.
15. Vinayaka KS, Karthik S, Nandini KC, Kedura TRP, Amylase inhibitory activity of some macrolichens of western ghats, Karnataka, India, Indian Journal of Novel Drug Delivery, 2013; 5(4):225–228.
16. Gulluce M, Aslan A, Sokmen M, Screening the antioxidant and antimicrobial properties of the lichens Parmelia saxatilis, Platismatia glauca, Ramalina pollinaria, Ramalina polymorpha and Umbilicaria nylanderiana, Phytomedicine, 2006; 13(7):515–521..
17. Luo H, Wei X, Yamamoto Y, Antioxidant activities of edible lichen Ramalina conduplicans and its free radical scavenging constituents, Mycoscience, 2010; 51(5):391–395.
18. Xia TY, Namujila L, Ying BH, Chemical constituents and their antioxidant activities from thallus of Ramalina conduplicans, Mycosystema, 2015; 34(1):169–176.
19. Rafika, B Monia A, Antibacterial Activity of the Chloroform, Acetone, Methanol and queous Extracts of Algerian Lichens, Jordan Journal of Pharmaceutical Sciences, 2018; 11(2):55-67.
20. Ankith GN, Rajesh MR, Karthik KN, Avinash HC, Prashith Kekuda, Vinayaka KS, Antibacterial and antifungal activity of three Ramalina species, Journal of Drug Discovery and Therapeutics, 2017; 7(5):27-32.
21. Lina A, Ghassan K, Mohammed W, Ahmed E, Efficacy of extracts of some lichens for potential antibacterial activity, Research journal of pharmaceutical, biological and chemical sciences, 2015; 6(1):318.
22. Igor ZS, Niko S, Radulovic, Tatjana L, Mitrovic, Slavisa M, Stamenkovic, Gordana S. Stojanovic, Volatile constituents of selected parmeliaceae lichens, Journal of the Serbian Chemical Society, 2011; 76(7):987–994.
23. Bisht S, Sharma S, Kumar V, Kumar M, Bisht S, Nautiyal BP, (2014), Assessment of antimicrobial efficacy of secondary metabolites of lichen species from uttarakhand temperate himalayas, Journal of natural products, 2014; 7:168-176.
24. Adriana B, Daniela R, Stefano L, Annalisa DS, Angela N, Sergio S, Barbara C, Luca P, Francesca DR, Anna MM, Lucia A, Paola B , Anti proliferative, antibacterial and antifungal activity of the lichen Xanthoria parietina and its secondary metabolite parietin, International Journal of Molecular Sciences, 2015; 16:7861-7875.
25. Kumar R, Thajuddin N, Upreti DK, Diversity of lichens in Kollihills of Tamil Nadu, India, International Journal of Biodiversity and Conservation, 2011; 3(2):36-39
26. Gayathri D, Swamy C, Lichens: A novel and potential source as antimicrobials for human use, Journal of phytology, 2012; 4(1): 38-43.
27. Junaid S, Rakesh KN, Dileep N, Poornima G, Kekuda PTR, Mukunda S, Total phenolic content and antioxidant activity of seed extract of Lagerstroemia speciosa L, Journal of Chemical Science Transactions,2013; 2(1):75-80.
28. Pratap CR., Vysakhi MV, Manju S, Kannan M, Abdul Kader S, Sreekumaran Nair A,In vitro free radical scavenging activity of aqueous and methanolic leaf Extracts of aegle tamilnadensis abdul kader (Rutaceae), International Journal of Pharmacy and Pharmaceutical Sciences, 2013; 5( 3):819-823.
2. Awasthi DD, The typification of Roccella montagnei, The Bryologist, 1981; 84(2):216--219.
3. Bauer AW, Kirby WMM., Sherris JC, Turck M, Antibiotic susceptibility testing by a standardized single disc method, American Journal of Clinical Pathology, 1966; 45(4):493.
4. Karthikaidevi G, Thirumaran G, Manivannan K, Anantharaman P, Kathiresan K, Balasubaramanian T, Antimicrobial activities of the lichen Roccella belangeriana (awasthi) from mangroves of mannar, Indian journal of geo-marine sciences, Vol. 2011; 40(3):449-453.
5. Rashmi S, Rajkumar HG, Preliminary phytochemical screening of different solvent extracts of lichens from kodagu district, Karnataka, Journal of pharmacognosy and phytochemistry, 2014; 3(4):209-212.
6. Revathy M, Sathya SS, Manimekala N, Annadurai G, Ahila M, Preliminary phytochemical investigation and antibacterial effects of lichen Parmotrema perlatum against human pathogens, European journal of biomedical and pharmaceutical sciences, 2015; 2:336-347.
7. Nayaka S, Methods and techniques in collection, preservation and identification of lichens. In plant Taxonomy and Biosystematics–Classical and Modern Methods (Eds T.S. Rana, K.N. Nair and D.K. Upreti). New India Publishing Agency, New Delhi 2014; 101-128.
8. Saliha S, Seyhan O, Pinar S, Cevdet D, Sule O, Ramalina lichens and their major metabolites as possible natural antioxidant and antimicrobial agents, Journal of Food Biochemistry, 2015; 3(9):471-477.
9. Poornima S, Ponmurugan P, Mythili Gnanamangai B, Nagarjun N, Ayyappadasan G. Evaluation of antimicrobial, antioxidant and anticancer activities of few macrolichens collected from Eastern Ghats of Tamil Nadu, India, International Research Journal of Pharmacy, 2017; 8(3):39-43.
10. Behra BC, Verma N, Sonone A, Makhjia U, Evaluation of antioxidant potential of the cultured mycobionts of a lichen Usnea ghattensis, Phytotherapy Research 2005; 19: 58-64.
11. Subhash Gaikwad, Neeraj Verma, Sharma BO, Behra BC, Growth promoting effects of some lichen metabolites on probiotic bacteria, Journal of Food Science and Technology 2012; 51(10):2624-2631.
12. Sharma BC, Kalikoty S, Screening of antioxidant activity of lichens Parmotrema reticulatum and Usnea sp. from Darjeeling hills, India, IOSR Journal of Pharmacy, 2012; 2(6): 54-60.
13. Choi Y, Jeong H, Lee J, Antioxidant activity of methanolic extracts from some grains consumed in Korea, Food Chemistry, 2007; 103:130-138.
14. Jayaprakasha GK, Rao LJ. Phenolic constituents from the lichen Parmotrema stuppeum (Nyl.) Hale and their antioxidant activity, A Journal of Biosciences, 2000; 55: 1018-1022.
15. Vinayaka KS, Karthik S, Nandini KC, Kedura TRP, Amylase inhibitory activity of some macrolichens of western ghats, Karnataka, India, Indian Journal of Novel Drug Delivery, 2013; 5(4):225–228.
16. Gulluce M, Aslan A, Sokmen M, Screening the antioxidant and antimicrobial properties of the lichens Parmelia saxatilis, Platismatia glauca, Ramalina pollinaria, Ramalina polymorpha and Umbilicaria nylanderiana, Phytomedicine, 2006; 13(7):515–521..
17. Luo H, Wei X, Yamamoto Y, Antioxidant activities of edible lichen Ramalina conduplicans and its free radical scavenging constituents, Mycoscience, 2010; 51(5):391–395.
18. Xia TY, Namujila L, Ying BH, Chemical constituents and their antioxidant activities from thallus of Ramalina conduplicans, Mycosystema, 2015; 34(1):169–176.
19. Rafika, B Monia A, Antibacterial Activity of the Chloroform, Acetone, Methanol and queous Extracts of Algerian Lichens, Jordan Journal of Pharmaceutical Sciences, 2018; 11(2):55-67.
20. Ankith GN, Rajesh MR, Karthik KN, Avinash HC, Prashith Kekuda, Vinayaka KS, Antibacterial and antifungal activity of three Ramalina species, Journal of Drug Discovery and Therapeutics, 2017; 7(5):27-32.
21. Lina A, Ghassan K, Mohammed W, Ahmed E, Efficacy of extracts of some lichens for potential antibacterial activity, Research journal of pharmaceutical, biological and chemical sciences, 2015; 6(1):318.
22. Igor ZS, Niko S, Radulovic, Tatjana L, Mitrovic, Slavisa M, Stamenkovic, Gordana S. Stojanovic, Volatile constituents of selected parmeliaceae lichens, Journal of the Serbian Chemical Society, 2011; 76(7):987–994.
23. Bisht S, Sharma S, Kumar V, Kumar M, Bisht S, Nautiyal BP, (2014), Assessment of antimicrobial efficacy of secondary metabolites of lichen species from uttarakhand temperate himalayas, Journal of natural products, 2014; 7:168-176.
24. Adriana B, Daniela R, Stefano L, Annalisa DS, Angela N, Sergio S, Barbara C, Luca P, Francesca DR, Anna MM, Lucia A, Paola B , Anti proliferative, antibacterial and antifungal activity of the lichen Xanthoria parietina and its secondary metabolite parietin, International Journal of Molecular Sciences, 2015; 16:7861-7875.
25. Kumar R, Thajuddin N, Upreti DK, Diversity of lichens in Kollihills of Tamil Nadu, India, International Journal of Biodiversity and Conservation, 2011; 3(2):36-39
26. Gayathri D, Swamy C, Lichens: A novel and potential source as antimicrobials for human use, Journal of phytology, 2012; 4(1): 38-43.
27. Junaid S, Rakesh KN, Dileep N, Poornima G, Kekuda PTR, Mukunda S, Total phenolic content and antioxidant activity of seed extract of Lagerstroemia speciosa L, Journal of Chemical Science Transactions,2013; 2(1):75-80.
28. Pratap CR., Vysakhi MV, Manju S, Kannan M, Abdul Kader S, Sreekumaran Nair A,In vitro free radical scavenging activity of aqueous and methanolic leaf Extracts of aegle tamilnadensis abdul kader (Rutaceae), International Journal of Pharmacy and Pharmaceutical Sciences, 2013; 5( 3):819-823.
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Soundararajan S, Shanmugam P, Nagarajan N, Palanisamy D, Ponnusamy P. In vitro study on screening antimicrobial and anti-oxidant potential of Ramalina fastigiata. JDDT [Internet]. 15Jan.2019 [cited 27Jan.2021];9(1):216-9. Available from: http://jddtonline.info/index.php/jddt/article/view/2217
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