Simultaneous estimation of gabapentin and methylcobalamin in bulk and pharmaceutical dosage form by RP-HPLC

  • Anjali Bakshi Department of Pharmaceutical Analysis and Quality Assurance, Sri Venkateshwara College of Pharmacy, Osmania University, Hyderabad -500081.
  • K Monika Department of Pharmaceutical Analysis and Quality Assurance, Sri Venkateshwara College of Pharmacy, Osmania University, Hyderabad -500081.
  • Shweta Bhutada Department of Pharmaceutics, Calcutta Institute of Pharmaceutical Technology & AHS, Banitabla, Uluberia, Howrah-711316, West Bengal.
  • M. Bhagvan Raju Department of Pharmaceutical Analysis and Quality Assurance, Sri Venkateshwara College of Pharmacy, Osmania University, Hyderabad -500081.

Abstract

A simple, selective, linear, precise, and accurate RP-HPLC method was developed and validated for the simultaneous estimation of Gabapentin & Methylcobalamin from bulk and formulation. Chromatographic separation was achieved Isocratically on an Inertsil C18 column (150x4.6, 5µ particle size) using a mobile phase Buffer: Acetonitrile in the ratio of 60:40 v/v. The flow rate was 1.0 ml/min, effluents were detected at 264 nm and 10µl of sample was injected. Retention time of Gabapentin & Methylcobalamin was found to be 2.7 and 4.13 min respectively. Linearity of the method was in the concentration range of 25-150 µg for Gabapentin & 0.125-0.750 µg for Methylcobalamin. Percent recoveries obtained for both the drugs were 100.00%. The percentage RSD for precision of the method was found to be less than 2%. The method was validated according to the ICH guidelines. The method developed was successfully applied for the analysis of simultaneous estimation of Gabapentin & Methylcobalamin tablets and was fairly good in comparison with other methods.


Keywords: Gabapentin, Methylcobalamin, HPLC.

Downloads

Download data is not yet available.

Author Biographies

Anjali Bakshi, Department of Pharmaceutical Analysis and Quality Assurance, Sri Venkateshwara College of Pharmacy, Osmania University, Hyderabad -500081.

Department of Pharmaceutical Analysis and Quality Assurance, Sri Venkateshwara College of Pharmacy, Osmania University, Hyderabad -500081.

K Monika, Department of Pharmaceutical Analysis and Quality Assurance, Sri Venkateshwara College of Pharmacy, Osmania University, Hyderabad -500081.

Department of Pharmaceutical Analysis and Quality Assurance, Sri Venkateshwara College of Pharmacy, Osmania University, Hyderabad -500081.

Shweta Bhutada, Department of Pharmaceutics, Calcutta Institute of Pharmaceutical Technology & AHS, Banitabla, Uluberia, Howrah-711316, West Bengal.

Department of Pharmaceutical Analysis and Quality Assurance, Sri Venkateshwara College of Pharmacy, Osmania University, Hyderabad -500081.

M. Bhagvan Raju, Department of Pharmaceutical Analysis and Quality Assurance, Sri Venkateshwara College of Pharmacy, Osmania University, Hyderabad -500081.

Department of Pharmaceutical Analysis and Quality Assurance, Sri Venkateshwara College of Pharmacy, Osmania University, Hyderabad -500081.

References

1) International Conference on Harmonization (ICH) of technical requirements for the registration of pharmaceuticals for human use, validation of analytical procedures: definitions and terminology, Geneva, 1996.
2) Sharma MC et al, Simultaneous Estimation and Validation of Gabapentin and Methylcobalamin in Tablet Dosage form: hydrotropic approach, Drug Invention Today, 2011; 3(6):95.
3) Baheti KG, Galande VR, Simultaneous Estimation of Gabapentin in the Presence of Methylcobalamin in Tablet by HPTLC Method, International journal of research in Pharmaceutical and Biomedical sciences, Jul-Sep 2011; 2(3).
4) Abdullatif HE, Khalil H.M., Colorimetric determination of Gabapentin in pharmaceutical formulation, J. Pharma. and Biomed. Anal, 2003.
5) Bharath BR, Shantha A., Malairajan P. et al. Simultaneous estimation and validation of Gabapentin and Methylcobalamin in combined dosage form by PR-HPLC, Indian Drugs, 2007; 44(10)L:784-788. 8. Beckett A. H. and Stenlake.
6) Galande R et al, UV VIS Spectrophotometric method for the estimation of Gabapentin & Mecobalamin in bulk & tablet, International Journal of Chemtech research, Jan – Mar 2010; 2(1):695-699
7) Lakshmi B et al, UV VIS Spectrophotometric method for the estimation of Gabapentin in formulations, The Experiment, August 2012; 2:84-92.
8) Goti PP, Patel PB, Development and validation of ratio derivative spectrophotometric method for simultaneous estimation of Gabapentin, Methylcobalamin and alpha lipoic acid in tablet formulation, Journal of Pharmacy research, june 2013; 6(6):
9) Saravanan V., Revathi R., Meera N. Method development and validation for the simultaneous estimation of lycopene and ubidecarenone by RP-HPLC in combined pharmaceutical dosage form. Journal of Drug Delivery and Therapeutics, 2016; 6(5):46-51.
10) Shashe Kumar P, Reddy R et.al. RP-HPLC method development and validation for simultaneous estimation of gabapentin and mecobalamine in tablet dosage form. IJPRA, 2014; 4(7):388-392.
11) Sumaiya H, Vani R et al, stability indicating method development and Validation of RP-HPLC method for simultaneous estimation of gabapentin and mecobalamine in bulk and its tablets; world journal of pharmacy and pharmaceutical sciences; 2014,3(12):1095-1106.
Statistics
149 Views | 94 Downloads
How to Cite
Bakshi, A., Monika, K., Bhutada, S., & Bhagvan Raju, M. (2019). Simultaneous estimation of gabapentin and methylcobalamin in bulk and pharmaceutical dosage form by RP-HPLC. Journal of Drug Delivery and Therapeutics, 9(1), 170-174. https://doi.org/10.22270/jddt.v9i1.2204