HPTLC fingerprinting of stem bark extract of Nyctanthes arbor-tristis (L.)
Fingerprint profile of bark extract of Nyctanthes arbor-tristis using High Performance Thin Layer chromatography (HPTLC) has been established. HPTLC is a valuable tool for the investigation of medicinal plants with reference to the qualitative analysis of the phytoconstituents. Separation of the active constituents from the extracts has been developed using solvent system of Toluene: Ethyl acetate: Formic acid (5:4:1). The HPTLC analysis showed the presence of the flavonoid quercetin in the standard as well as in the sample and the Rf value was 0.73. These images of fingerprinting help in the proper identification and quantification of the marker compounds. On the basis of the marker compounds, new drugs could be formulated to treat various diseases
Keywords: Nyctanthes arbor- tristis, HPTLC analysis, quercetin, bark extract
2. Tuntiwachwuttiku P, Rayanil K, Taylor WC. Chemical constituents from the flowers of Nyctanthes arbortristis. Science Asia, 2003; 29:21- 30.
3. CAMAG, 2010-2011. Basic equipment for modern thin layer chromatography. Switzerland: Camag. Available from: camag.com/downloads/free/brochures/CAMAG-basic-equipment- 08.
4. SachinRakesh, U., Patil, PR., Salunkhe, VR., Dhabale, PN and Burade, KB. HPTLC method for quantitative determination of quercetin in hydroalcoholic extract of dried flower of Nymphaea stellatawilld, International Journal of ChemTech Research. 2009; 1(4):931-936
5. Kim, SY., Kim, JH., Kim, SK., Oh, MJ and Jung, MY. Antioxidant activities of selected oriental herb extracts. Journal of the American Oil Chemists' Society.vol.1994; 71(6):633–640.
6. Hertog, MGL., Feskens, EJM., Hollman, PCH., Katan, JB and Kromhout, D. Dietary antioxidant flavonoids and risk of coronary heart disease. The Zutphen Elderly Study, The Lancet, 1993; 342:1007–1011.
7. Barakat, MZ.,Shahab,SK.Darwin,N and Zahemy,EI. Determination of ascorbic acid from plants, Analytical Biochemistry, 1993; 53:225–245.
8. JyothiGowda, S and Somashekaraiah Veerabhadrappa, B. Study of in vitro antioxidant activity and HPTLC fingerprint of quercetin in Cassia auriculata L. Asian Journal
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).