Physico-chemical Standardization of NPCF (Non-Pharmacopoeial Compound Formulation) Use in Diabetes mellitus
Background: Variety of reasons has been cited for the need for scientific evaluation and standardization of herbal drugs. Three methods, viz., activity-based standardization, determination of biologically active compound and standardization of herbal drugs on various physical, chemical and other parameters were found to be suitable and practically applicable. Phytochemical investigations along with biological screening to understand the therapeutic dynamics of medicinal plants etc. will help in developing quality parameters and help in the standardization and establish for authenticity and quality. Material and methods: Standardization was made on the basis of physicochemical and analytical parameters laid down by National Unani Pharmacopoeia Committee (Anonymous, 2007). Results: The parameters studied includes alcohol soluble content 15.34±0.48 water soluble content 18.27±0.38, successive extractive values viz. petroleum ether 7.60±0.05 , diethyl ether 1.24±0.02, chloroform 2.24± 0.04 , acetone 4.74±0.02, alcohol 8.51±0.06 , and aqueous 4.20±0.05, non- successive extractive values viz. petroleum ether 6.70±0.06, chloroform 7.70±0.06, alcohol16.15± 0.03 and aqueous 10.65±0.03, total ash 5.50±0.28 , acid insoluble ash 2.33±0.33, water soluble ash 1.16±0.16 , moisture content 7.30±0.12 , Bulk density 0.419±0.005 (pour density) and 0.74±0.01 (Tap density), pH values of 1% solution 4.89±0.01 and 10% solution 4.69±0.005. The qualitative analysis of various phytochemicals was estimated that revealed the presence of alkaloid, carbohydrate, flavonoids, protein, starch, phenols, tannin, sterols, amino acid and resin. The TLC profile of the extracts of non-pharmacopoeial Compound Formulation was also performed which confirms various biomolecules in it.. Conclusion: This study helps in determining the quality and purity of NPCF which is use in Diabetes mellitus.
Keywords: Standardization, Non-Pharmacopoeial Compound formulation, Physico-chemical study
2. Benchmarks for training in traditional / complementary and alternative medicine: Benchmarks for training in Unani medicine. Geneva: World Health Organization. 2010. 20 p. ISBN 978 92 4 159964
3. Rasheed N.M.A., V.C.Gupta. Standardization of a compound Unani herbal formulation “Qurs-e-Luk” with modern techniques. Pharmacognosy Research. 2010; 2(4)237-241.
4. Elvin-Lewis M. Should we be concerned about herbal remedies. J Ethnopharmacol 2001; 75:141-64.
5. Ali A, Sumbul S, Abdin MZ, et al. Development of standard operating procedure and standardization of Habbe-Banafsha Qawi-A Unani polyherbal formulation. J Pharm Bioallied Sci 2015; 7:250-3.
6. Yaroshenko IS, Kirsanov DO, Wang P, et al. Determination of the toxicity of herb preparations of the traditional Chinese medicine with a multisensor system. Russian Journal of Applied Chemistry 2015; 88:72-81.
7. Abdel aziz A, Sushko Y, Novotarskyi S, et al. Using Online Tool (iPrior) for Modeling ToxCast Assays Towards Prioritization of Animal Toxicity Testing. Comb Chem High Throughput Screen 2015; 18:420-38.
8. Youns M, Hoheisel JD, Efferth T. Toxicogenomics for the prediction of toxicity related to herbs from traditional Chinese medicine. Planta Med 2010; 76:2019-25.
9. Shi J, Cao B, Wang XW, et al. Metabolomics and its application to the evaluation of the efficacy and toxicity of traditional Chinese herb medicines. J Chromatogr B Analyt Technol Biomed Life Sci 2016; 1026: 204-16.
10. Li CY, Song HT, Wang XX, et al. Urinary metabolomics reveals the therapeutic effect of HuangQi Injections in cisplatin-induced nephrotoxic rats. Sci Rep 2017; 7:3619.
11. Zhang H, Fu P, Ke B, et al. Metabolomic analysis of biochemical changes in the plasma and urine of collageninduced arthritis in rats after treatment with Huang-LianJie-Du-Tang. J Ethnopharmacol 2014; 154:55-64.
12. Afaq SH, Tajuddin, Siddiqui MMH. Standardization of Herbal Drugs. Publication
13. Jenkins GL, Knevel AM and Digangi FE. Quantitative Pharmaceutical Chemistry, 6th edition. CBS Publishers and Distributors Pvt. Ltd. New Delhi, India; 2008.p. 225, 235, 425,432,476,492, 496.
14. Anonymous. Pharmacopoeia of India, 2nd edition, Government of India, Ministry of Health, Manager of Publications, Delhi, India; 1970. P.110, 277,441.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeÂ The Effect of Open Access).