Phytochemical screening and GC–MS analysis of bioactive compounds present in ethanolic leaves extract of Silybum marianum (L).

  • M Padma P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.
  • S Ganesan P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.
  • T Jayaseelan P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.
  • S Azhagumadhavan P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.
  • P Sasikala P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.
  • S Senthilkumar P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.
  • P Mani Dept. of Biotechnology, Annai College of Arts and Science, Kumbakonam, Tamilnadu, India.

Abstract

Objective: To investigate the phytochemicals and GC-MS analysis of ethanol extracts of Silybum marianum.


Methods: The air-dried leaves were powdered and subjected to selective sequential extraction using solvents of increasing polarity through percolation, ethanol to obtain an ethanolic extract. Then, each of the extracts was further subjected to gas chromatography-mass spectrometry.


Results: Qualitative determination of the different biologically active compounds from crude extracts of Silybum marianum using gas chromatography-mass spectrometry revealed different types of high and low molecular weight chemical entities with varying amounts present in each of the extracts. These chemical compounds are considered biologically and pharmacologically important.


Conclusions: The study established the chemical composition and anticancer activity of the plant.


Keywords: Silybum marianum, Phytochemicals screening, GC–MS analysis, Bioactive compounds.

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Author Biographies

M Padma, P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.

P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.

S Ganesan, P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.

P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.

T Jayaseelan, P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.

P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.

S Azhagumadhavan, P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.

P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.

P Sasikala, P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.

P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.

S Senthilkumar, P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.

P.G. and Research Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi - 613 503, Thanjavur District, Tamil Nadu, India.

P Mani, Dept. of Biotechnology, Annai College of Arts and Science, Kumbakonam, Tamilnadu, India.

Dept. of Biotechnology, Annai College of Arts and Science, Kumbakonam, Tamilnadu, India.

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Padma, M., Ganesan, S., Jayaseelan, T., Azhagumadhavan, S., Sasikala, P., Senthilkumar, S., & Mani, P. (2019). Phytochemical screening and GC–MS analysis of bioactive compounds present in ethanolic leaves extract of Silybum marianum (L). Journal of Drug Delivery and Therapeutics, 9(1), 85-89. https://doi.org/10.22270/jddt.v9i1.2174