Anti-inflammatory activity and phytochemical analysis of Moringa oleifera ethanol and acetone leaves extract
This present investigation stated that acetone and ethanol extract of M,oleifera leaves was estimated that presence of phytochemical constituents by biochemical test and evaluated for anti-inflammatory activity. The anti-inflammation activity was assessed by calculating inhibition of protein denaturation, proteinase activity and membrane stabilization activity at different concentration of extract. The plant extract highly protective activity against heat induced protein denaturation and the IC50 results values 271.25±2.74 and 304.25±2.33μg/ml, for acetone and ethanol extract respectively. Heat induced haemolysis was 50% inhibited for acetone and ethanol extract at the concentration of 271.43±0.73 and 322.10±1.34 μg/ml, respectively. The membrane stabilization activity (IC50) was assessed by hypotonicity induced haemolysis at a concentration of 216.98±1.84 and 259.65±1.83μg/ml for acetone and ethanol extract, respectively. The results obtained in the present study indicate that ethanol extracts of M.oleifera leaves can be a potential source of anti-inflammatory agents compared than acetone extract and standard drug.
Keywords: Antinflammatory, plant extract, phytochemicals
2. Cotran RS, Kumar V, Robbins SL. In: Robbins,Pathologic Basis of Disease. Philadelphia: W.B.Saunders Company; 1994.
3. Leelaprakash G, Mohan Dass S, Invitro Anti-Inflammatory activity of Methanol extract of Enicostemma Axillare, Int J Drug Dev & Res, 2011; 3(3):189-196
4. Unuigbe CA, Henry A. Okeri, Osayemwenre Erharuyi, Emmanuel E. Oghenero and Dominic A. Obamedo, Phytochemical and antioxidant evaluation of Moringa oleifera (Moringaceae) leaf and seed, J. Pharmacy & Bioresources 2014; 11(2):51-57
5. Farnsworth NR, Soejarto DD, Global importance of medicinal plants, Akereleo, Heywood V and synge H (eds.). CambridgeU.press, Cambridge, UK. Conservation ofmedicinal plants, 1991; 2:25-51.
6. Iqbal, S., Bhanger, M.I. (2006) Effect of season and production location on antioxidant activity of Moringa oleifera leaves grown in Pakistan. J. of Food Comp. and Anal 19:544-551.
7. Abdulkarima, S.M., Long, K., Lai, O.M., Muhammad, S.K.S., Ghazali, H.M. Frying quality and stability of high-oleic Moringa oleifera seed oil in comparison with other vegetable oils. Food Chemistry 2007; 105:1382–1389.
8. Kasolo JN, Gabriel S Bimenya, Lonzy Ojok, Joseph Ochieng and Jasper W Ogwal-Okeng, Phytochemicals and uses of Moringa oleifera leaves in Ugandan rural communities, Journal of Medicinal Plants Research 2010; 4(9):753-757
9. Anwar, F., S. Latif, M. Ashraf and A.H. Gilani, Moringa oleifera: a food plant with multiple medicinal uses. Phytotherapy Research 2007; 21:17-25.
10. Mashiar M., I. Mominul, A. Sharma, I. Soriful, R. Atikur, R. Mizanur and M. Alam. Antibacterial activity of leaf juice extracts of Moringa oleifera Lam. against some human pathogenic bacteria. Chiang Mai University Journal of Natural Sciences Nat. Sci. 2009; 8:219-227.
11. Torres-Castillo JA, SR Sinagawa-García, GCG Martínez-Ávila et al, Moringa oleifera: phytochemical detection, antioxidants, enzymes and antifungal properties, YTON 2013; 82:193-202
12. Harborne, J.B. Phytochemical Methods. Springer (India) Pvt. Ltd., New Delhi, 2005; 17:49-188.
13. Trease GE and Evans WC, Pharmacognosy. 13thedn. Bailliere Tindall,London, 1989; 176-180.
14. Sazada S, Verma A, Rather AA, Jabeen F, Meghvansi MK, Preliminary phytochemicals analysis of some important medicinal and aromatic plants. Adv in Biol Res, 2009; 3:188-195
15. Mizushima Y and Kobayashi M, Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins J of Pharma Pharmacol 1968; 20:169-173.
16. Oyedepo OO and Femurewa AJ. Anti-protease and membrane stabilizing activities of extracts of Parham P. The immune system. New York: Garland Publishing; 2000.
17. Sadique J, Al-Rqobahs WA, Bughaith, EIGindi Ar, The bioactivity of certain medicinal plants on the stabilization of RBS membrane system. Fitoterapia. 1989; 60:525-532.
18. Sakat S, Juvekar AR, Gambhire MN, In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. International Journal of Pharma and Pharmacological Sciences 2010; 2(1):146-155.
19. Shinde UA, Kulkarni KR, Phadke AS, Nair AM, Dikshit VJ, Mungantiwar, Saraf MN, Mast cell stabilizing and lipoxygenase inhibitory activity of Cedrus deodara (Roxb.) Loud. Wood Oil. Indian J Exp Biol , 1999; 37(3):258-261.
20. Azeem AK, Dilip C, Prasanth SS, Junise V, Hanan Shahima, Anti-inflammatory activity of the glandular extracts of Thunnus alalunga. Asia Pac J for Med 2010; 3(10):412-20.
21. Zhang LL, Lin YM, Tannins from Canarium album with potent antioxidant activity. J. Zhejiang Univ Sci B, 2008; 9:407-415.
22. Yoshida T, Konishi M, Horinaka M,Yasuda T, Goda AE, Taniguchi H, YanoK, Wakada M, Sakai T, Kaempferol sensitizes colon cancer cells to TRAILinduced apoptosis. Biochem and BiophysRes Comm. 2008; 375:129-133.
23. Kaur GJ, Aroara DS, Antibacterial and Phytochemical screening of Anthum graveolens, Foeniculum vulgare andTrachyspermum ammi. BMC Complement Altern. 2009; 9:30.
24. Fawole OA, Amoo SO, Ndhlala AR, Light ME, Finnie JF, Van Staden J, Anti-inflammatory, anticholinesterase, antioxidant and phytochemical properties of medicinal plants used for pain-related ailments in South Africa. J Ethnopharmacol, 2010; 127(2):235-241
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).