Extraction, Qualitative and Quantitative Determination of Secondary Metabolites of Rumex Nepalensis Roots
In the Indian ayurvedic system of medicine, Rumex nepalensis Spreng. (Polygonaceae) commonly known as Nepal Dock has wide-spectrum therapeutic potencies and is extensively used for centuries in traditional medicine systems. They act as a possible food supplement and are largely used in pharmaceutical industry. Extracts and metabolites from this plant exhibits pharmacological activities including anti-inflammatory, antioxidant, antibacterial, antifungal, antiviral, insecticidal, purgative, analesic, antipyretic, anti-algal, central nervous system depressant, genotoxic, wound healing and skeletal muscle relaxant activity. Due to its remarkable biological activities, it has the potential to act as a rich source of drug against life threatening diseases. The aim of the present study is to examine Rumex nepalensis roots for phytochemical profile. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method and aluminium chloride method respectively. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids, fixed oil and fats. The total phenolics content of roots ethanolic extract was (1.658 mg/100mg), followed by flavonoids (1.048mg/100mg). The present study concluded that the crude extract of Rumex nepalensis is a potential source of various activates and this justifies its use in folkloric medicine.
Keywords: Rumex nepalensis, Qualitative analysis, Quantitative analysis, TPC, TFC, Folins Ciocalteau
2. Farooq U, Pandith SA, Saggoo MI, Lattoo SK. Altitudinal variability in anthraquinone constituents from novel cytotypes of Rumex nepalensis Spreng. a high value medicinal herb of North Western Himalayas. Ind Crops Prod 2013; 50(10):112-117.
3. Ahmad KS, Kayani WK, Hameed M, Ahmad F, Nawaz T. Floristic diversity and ethnobotany of Senhsa, District Kotli, Azad Jammu & Kashmir (Pakistan). Pak J Bot 2012; 44:195-201.
4. Yi YL, Lei Y, Yin YB, Zhang HY, Wang GX. The antialgal activity of 40 medicinal plants against Microcystis aeruginosa. J Appl Phycol 2012; 24(4):847-856.
5. Ankita J, Jain A. Tridax procumbens (L.): A weed with immense medicinal importance: A review. Int J Pharma Bio Sci 2012; 3(1):544- 552.
6. Anusuya NA, Gomathi RA, Manian SE, Sivaram VE, Menon AN. Evaluation of Basella rubra L., Rumex nepalensis Spreng and Commelina benghalensis L. for antioxidant activity. Int J Phar Pharmaceut Sci 2012; 4(3):714-720.
7. Kensa. M. Floristic study in a Vembanur wetland, Kanyakumari District, Tamilnadu, South India. Plant Sci Feed 2011; 1:194-199.
8. Farooquee NA, Majila BS, Kala CP. Indigenous knowledge systems and sustainable management of natural resources in a high altitude society in Kumaun Himalaya, India. J Hum Ecol 2004; 16(1):33-42.
9. Gaur RD. Traditional dye yielding plants of Uttarakhand, India. Nat Prod Radiance 2008; 7(2):154-165.
10. Liang HX, Dai HQ, Fu HA, Dong XP, Adebayo AH, Zhang LX, et al. Bioactive compounds from Rumex plants. Phytochem Lett 2010; 3(4):181-184.
11. Wahid SF, Osman CP, Ismail NH. Distinguishing isomeric anthraquinone by LC-MS. Global J Pharmacol 2013; 7(4):479-485.
12. Kumar SU, Joseph L, George MA, Bharti VI. Antimicrobial activity of methanolic extracts of Rumex nepalensis leaves. Int J Pharm Pharm Sci 2011; 3(4):240-242.
13. Aggarwal PK, Kumar LA, Garg SK, Mathur VS. Effect of Rumex nepalensis extracts on histamine, acetylcholine, carbachol, bradykinin, and PGs evoked skin reactions in rabbits. Ann Allergy 1986; 56(2):177-182.
14. Solanki R, Dalsania S. Evaluation of CNS action of Rumex nepalensis Spreng. (Polyginaceae) using mice as experimental animal. Int J Res Pharm Biomed Sci 2012; 3:1750-1752.
15. Kumar S, Joseph L, George M, Kaur L, Bharti V. Skeletal muscle relaxant activity of methanolic extract of Rumex nepalensis in albino rats. J Chem Pharm Res 2011; 3(3):725-728.
16. Sneader W, The discovery of aspirin: A reappraisal, BMJ (Clinical research ed., 2000; 321 (7276):1591-1594.
17. Aguoru CU, Ameh SJ, Olasan O, Comparative phyochemical studies on the presence and quantification of various bioactive compounds in the three major organs of okoho plant (cissus popunea guill & perr) in Benue State, North Central Nigeria, West Africa, European Journal of Advanced Research in Biological and Life Sciences, 2014; 2 (2):16-22.
18. Mukherjee PK. Quality control of herbal drugs. 2nd Ed. Business Horizons; 2007.
19. Khandelwal KR. Practical pharmacognosy technique and experiments. 23rd Ed. Nirali Prakashan; 2005.
20. Kokate CK. Practical pharmacognosy. 4th Ed. Vallabh Prakashan; 1994.
21. Olufunmiso OO, Afolayan AJ, Phenolic content and antioxidant property of the bark extract of Ziziphus mucronata willd. Subsp. mucronata willd, BMC Complement Alternative Medicine, 2011; 11:130.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).