In-vitro cytotoxicity of java tea mediated selenium nanoballs against L6 cell lines
The emergence of Nanotechnology has provided a catholic research in recent years by intersecting with assorted branches of science and forming collision on all forms of life. At present there has been a prodigious excitement in the field of Nano pharmacology to seek the role of Nano selenium in individual healthcare and explains how selenium is a double edged sword in the pathologies of chronic diseases like Diabetes mellitus because of their inferior toxicity and ability to gradually release the bioactive principle and free selenium after ingestion. Thus the present study is an embryonic attempt and was aimed at the adroitful synthesis of selenium nanoballs from the aqueous extract of well known herbal tea leaves namely Java tea (Orthosiphon stamineus) using selenious acid solution. The synthesized Selenium nanoparticles were then subjected to various characterization techniques such as UV, FTIR, FESEM, EDAX and Zeta potential respectively. Finally the green synthesized SeNps were tested for their cytotoxic effect against L6 rat skeletal muscle cell lines. The pre clinical studies are underway to prove the insulin mimic activity of the selenium nanoparticles.
Keywords: Selenium Nanoparticles, Java tea, L6 cell lines.
2. Prasad KS, Pathak D, Patel A, Dalwadi P, Prasad R, Patel P, Selvaraj K.P. Biogenic synthesis of silver nanoparticles using Nicotiana tobaccum leaf extract and study of their antibacterial effect. Afr.J. Biotechnol, 2011; 10: 8122.
3. Sathishkumar G, Gobinath C, Karpagam K, Hemamalini V, Premkumar K, Sivaramakrishnan S, Phyto-synthesis of silver nanoscale particles using Morinda citrifolia L. and its inhibitory activity against human pathogens.Colloids Surf, 2012; B 95:235.
4. Murray CB, Kangan CR, Bawendi MG.Synthesis and characterization of monodisperse nanocrystals and close-packed nanocrystal assemblies. Annu. Rev. Mater. Sci. 2000; 30:545.
5. Ayyub P, Chandran R, Taneja RP, Sharma A, Pinto R. Synthesis of nanocrystalline material by sputtering and laser ablation at low temperature. Appl. Phys. A, 2001; 73:67.
6. Ren F, He X, Wang K, Yin JJ. Biosynthesis of gold nanoparticles using Catclaw Buttercup (Radix Ranunculi Ternati) and evaluation of its colloidal stability, Biomed.Nanotechnol, 2012; 8:586.
7. Zhang Y, Zhang J, Wang HY, Chen HY. Synthesis of selenium nanoparticles in the presence of polysaccharides. Lett, 2004; 58: 2590.
8. Yost DA, Russel JC, Yang H, Evidence on the size-dependent absorption spectral evolution of selenium nanoparticles U.S. Patent 4954452 (1990).
9. Sasidharan S, Balakrishnaraja R. Comparison studies on the synthesis of selenium nanoparticles by various microorganisms.Int. J. Pure. Appl. Biosci, 2014; 2:112-117.
10. Li, Shen SK, Xie YH, Yu AJ, Zhang XR, Yang XZ, Li LB, Rapid, room-temperature synthesis of amorphous selenium/protein compositesusing Capsicum annuumL. extract.Nanotechnol, 2007; 18:405101–405109.
11. Sharma G, Sharma AR, Bhavesh R, Park J, Ganbold B, Nam J, Lee S. Biomolecule-mediated synthesis of selenium nanoparticles using dried Vitisvinifera (raisin) extract. Molecules.2014; 19:2761-2770.
12. Prasad KS, Patel H, Patel T, Patel K, Selvaraj K. Biosynthesis of Se nanoparticles and its effect on UV-induced DNA damage.Colloids Surf. B. Biointerfaces, 2013; 103:261-266.
13. Prasad KS, Selvaraj K, Biogenic synthesis of selenium nanoparticles and their effect on As(III)-induced toxicity on human lymphocytes. Biol Trace Elem Res, 2014, 157:275-283.
14. Ramamurthy CH, Sampath KS, Arunkumar P, Kumar M.S, Sujatha V, Premkumar K, Thirunavukkarasu C. Green synthesis and characterization of selenium nanoparticles and its augmented cytotoxicity with doxorubicin on cancer cells. Bioprocess Biosyst Eng, 2013; 36:1131-1139.
15. Ganesan V, et al., Bioinspiredsynthesis of selenium nanoparticles using flowers of Catharanthus roseus(L.) G.Don.and Peltophorum pterocarpum(DC.)Backer ex Heyne – a comparison. Int.J. ChemTech Res. 2015; 7(2):725-733
16. Ganesan V. Biogenic synthesis and characterization of selenium nanoparticles using the flower of Bougainvillea spectabilis Willd. Int J Sci Res, 2015; 4:690–695.
17. Chin JH, Abas HH, Sabariah I. Effect of Orthosiphon stamineus leaf extracts on hepatic cytochrome P450, UGT and GST activity in STZ-induced diabetic rats. J Adv Sci Arts 2009; 1:1-8.
18. Suresh A, Yasuhiro T, Arjun H, Banskota S, Shimoji KT, Shigetoshi K. Siphonols A-E: Novel Nitric Oxide Inhibitors from Orthosiphon stamineus of Indonesia. Bioorg Med Chem Lett 2003; 13:31-35.
19. Bwin DM, Gwan US. Ministry of Health, Health and Myanmar traditional medicine. In: Burmese Indigenous Medicinal Plant: 1. Plants with reputed Hypoglycemic Action; Burma Medical Research Institute: Yangon, Burma, 1967; 126-28.
20. Eisai PT. Indonesia Medicinal Herb Index in Indonesia, 2nd ed. University Press: Godjah Mada, 1995: 239-263.
21. Tran K. WHO Regional Office for the Western Pacific Manila and Institute of Material Medica Hanoi, in Medicinal Plants in Vietnam; Science and Technology Publishing House: Hanoi, Vietnam 1970.
22. Dhanjal S, Cameotra SS. Aerobic biogenesis of selenium nanospheres by Bacillus cereus isolated from coalmine soil. Microbial Cell Factories, 2010; 9:52-61.
23. Oremland RS, Herbal MJ, Blum JS, Langely S, Beveridge TJ, Ajayan PM, Sutto T, Ellis AV. Structural and Spectral Features of Selenium Nanospheres Produced by Se-Respiring Bacteria,Appl. Environ. Microbiol, 2004; 70:52.
24. Doan DD, Nguyen NH, Doan HK. Studies on the individual and combined diuretics effects of four Vietnamese traditional herbal remedies (Zea mays, Imperata cylindrica, Plantago major and Orthosiphon stamineus). J Ethnopharmacol, 1992; 36(3):225-231.
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