Wound healing promoting activity of Earthworm, Eutyphoeus gammiei (Beddard): in vitro studies on human skin keratinocyte cell line (HaCat).

  • Susmita Saha Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.
  • Deepjyoti Bhattacharjee Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.
  • Anwesha Saha Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.
  • Gahin De Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.
  • Partha Saha Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.
  • S K Sil Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.
DOI https://doi.org/10.22270/jddt.v8i6.2036

Abstract

Earthworm, Eutyphoeus gammiei, homogenate (EGH) was screened for wound healing activity on human keratinocyte cell line, HaCat, by cell proliferation and migration assays. The maximum proliferation and migration of keratinocyte cells were observed at the dose of 25μg/ml. As cell proliferation and migration are key factors for wound healing, the study clearly suggests the potential role of earthworm species Eutyphoeus gammiei on wound healing.


Keywords: Eutyphoeus gammiei, Keratinocyte, MTT assay, scratch assay.

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Author Biographies

Susmita Saha, Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Deepjyoti Bhattacharjee, Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Anwesha Saha, Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Gahin De, Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Partha Saha, Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

S K Sil, Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

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1.
Saha S, Bhattacharjee D, Saha A, De G, Saha P, Sil S. Wound healing promoting activity of Earthworm, Eutyphoeus gammiei (Beddard): in vitro studies on human skin keratinocyte cell line (HaCat). JDDT [Internet]. 15Nov.2018 [cited 30May2020];8(6):155-8. Available from: http://jddtonline.info/index.php/jddt/article/view/2036
Issue
Vol 8 No 6 (2018): VOLUME 8, ISSUE 6 Nov-Dec 2018
Section
Research
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