Croton sparsiflorus whole plant extract mediated biosynthesis of metallic silver nanoparticles and their antibacterial activity

  • Pious Tresina Soris Ethnopharmacology Unit, PG & Research Department of Botany, V.O.Chidambaram College, Tuticorin, Tamilnadu, India
  • Gnanaraj Gnana Priyanka Beulah Ethnopharmacology Unit, PG & Research Department of Botany, V.O.Chidambaram College, Tuticorin, Tamilnadu, India
  • Asirvatham Doss Ethnopharmacology Unit, PG & Research Department of Botany, V.O.Chidambaram College, Tuticorin, Tamilnadu, India
  • Veerabahu Ramasamy Mohan Ethnopharmacology Unit, PG & Research Department of Botany, V.O.Chidambaram College, Tuticorin, Tamilnadu, India

Abstract

In the present study, the potential of aqueous whole plant extract of Croton sparsiflorus for biosynthesis of silver nanoparticles (AgNPs) was evaluated. The formation of AgNPs was confirmed by color changes and UV- Visible spectroscopy, which showed absorbance maxima peak at 417nm. The scanning electron microscope (SEM) analysis confirmed the particle size and x-ray diffraction (XRD) confirmed the crystalline character of AgNPs. AFM analysis also confirmed the surface morphology of synthesized AgNPs. Fourier transform infrared (FTIR) authorized the presence of alcoholic and phenolic groups played an important reduction role in the synthesis process. It shows the antibacterial efficiency against gram negative bacteria, Klebsiella pneumoniae and Salmonella paratyphi. From the results, it is suggested that green synthesized AgNPs could be used effective in future biomedical engineering.


Keywords: Croton sparsiflorus, Green synthesis, Silver nanoparticles, X-ray diffraction, Antibacterial activity.

Downloads

Download data is not yet available.

Author Biographies

Pious Tresina Soris, Ethnopharmacology Unit, PG & Research Department of Botany, V.O.Chidambaram College, Tuticorin, Tamilnadu, India

Ethnopharmacology Unit, PG & Research Department of Botany, V.O.Chidambaram College, Tuticorin, Tamilnadu, India

Gnanaraj Gnana Priyanka Beulah, Ethnopharmacology Unit, PG & Research Department of Botany, V.O.Chidambaram College, Tuticorin, Tamilnadu, India

Ethnopharmacology Unit, PG & Research Department of Botany, V.O.Chidambaram College, Tuticorin, Tamilnadu, India

Asirvatham Doss, Ethnopharmacology Unit, PG & Research Department of Botany, V.O.Chidambaram College, Tuticorin, Tamilnadu, India

Ethnopharmacology Unit, PG & Research Department of Botany, V.O.Chidambaram College, Tuticorin, Tamilnadu, India

Veerabahu Ramasamy Mohan, Ethnopharmacology Unit, PG & Research Department of Botany, V.O.Chidambaram College, Tuticorin, Tamilnadu, India

Ethnopharmacology Unit, PG & Research Department of Botany, V.O.Chidambaram College, Tuticorin, Tamilnadu, India

References

1. Mohanpuria P, Rana NK, Yadav SK. Biosynthesis of nanoparticles, technological concepts and future applications. Journal of Nanoparticle Research. 2007; 10: 507-517.
2. Thakkar KN, Mahatra SS, Parikh RK. Biological synthesis of metallic nanoparticles. Nanomadicine and Nanotechnology. 2010; 6:257-262.
3. Sharma VK, Yngard RA, Lim, Y. Silver nanoparticles green synthesis and their antimicrobial activities. Advances Colloid Interface Science. 2008; 145:83-96.
4. Dipankar C, Murugan S. The green synthesis, characterization and evaluation of the biological activities of silver nanoparticles synthesized from Iresineherbstii leaf aqueous extracts. Colloid surf B: Biointes 2012; 98:112-119.
5. Sivalingam P, Antony JJ, Siva D, Achiraman S, Ambarasam K. Mangrove Streptomyces sp. BDUKASIO as nanofacting for fabrication of bactericidal silver nanoparticles. Colloid Sur B: Biometes 2012; 98:12-17.
6. Kalimuthu K, Suresh Babu R, Venkataraman D, Bilal, M, Gurunatham, S. Bio synthesis of silver nanoparticles by Bacillus licheniformis. Colloid Surf B: Biointutace 2008; 65:150-153.
7. Ingle A, Gade A, Pierrat S, Sonnichsen C, Rai M. Mycosynthesis of silver nanoparticles using the fungus Fusarium acuminatum and its activity against some human pathogenic bacteria. Current Nanosis 2008; 4:141-144.
8. Meng X, Wang Z, Hui LV. Constituents and bacterio static activity of volatile matter from four power plant species. Indian Journal of Agriculture Research. 2010; 44:157-167.
9. Brindha P, Sasikala B, Purushothaman KK. Pharmacognostic studies on Merugan kizhangu. Bullutin Medicine. Ethno Botanical Research. 1989; 3:84-96.
10. Anonymous Indian Pharmacopoeia, vol. I & II, Government of India. Ministry of Health and family welfare. The controller of publication. Civil lines, Delhi-110054. 1996.
11. Lala PK Lab Manuals of Pharmacognosy. 5th Edition, CSI Publishers and distributors, Calcutta, 1993.
12. Hulther E, Fendler JH. Explostation of localized surface plasmon resonance. Advances Materials. 2004; 16:1688-1706.
13. Sun S, Murray C, Weller D, Folks L, Mosar. Monodisperse Fe pt nanoparticles and ferromagnetic Fe pt nanocrystal superlattices. Science. 2000; 267:1989-1992.
14. Vilchis-Nestor AR, Sanchez–Mendieta V, Camacho–Lopaz MA, Comez–Espinosa RM, Camacho-Lopez MA, Arenas–Alatorre JA. Solventless synthesis and opticle properties of Au & Ag nanoparticles using Camellia sinensis extrac.t Materials Letters. 2008; 67:3103-3105.
15. Zhang W, Qiao X, Chem J, Wany H. Preparation of silver nanoparticles in waher in oil AOT reverse micelles. Journal of Colloidal Interface Science. 2006; 302:170-173.
16. Chimentao RJ, Kirm I, Medina F, Rodriguez X, Cesteros Y, Salagre P, Sueiras JE. Different morpholopies of silver nanoparticles as catalysts for the selective oxidation of styrene in the gas phase. Chemical Communication. 2004; 4:846-847.
17. Muthukrishnan S, Bhaky S, Senthilkumar T, Rao. MV. Biosynthesis, Characterization and antibacterial effect of plant mediated silver nanoparticles using Ceropegia thwaitesii – An endemic species. Indian Crop Production. 2015; 63:119-124.
18. Patil MP, Kim GD. Eco friendly approach for nanoparticles synthesis and mechanism behind antibacterial activity of silver and anticancer activity of gold nanoparticles. Applied Microbiology and Biotechnology. 2017; 101:79-92.
19. Venkatesan J, Kim SK, Shim MS. Antimicrobial, antioxidant and anticancer activities of biosynthesized silver nanoparticles using marine algae Ecklonic cava Nanometer. 2017; 6:235.
20. Ahmed S, Ahmed M, Swami BI, Ikram S. A review on plant extract medicated synthesis of silver nanoparticles for antimicrobial applications a green expertise. Journal of Advances Research. 2016; 7:17-28.
21. Kumaraswamy M, Sudipta KM, Jayanta K, Bala Subramanya S. The green synthesis, characterization, and evaluation of the biological activities of silver nanoparticles synthesized from Leptadenia reticulata leaf extract. Applied Nanoscience. 2015; 5:73-81.
22. Paoren S, Saising J, Septama AW, VoravuhiKunchai SP. Green synthesis of silver nanoparticles using plants from Myrtaceae family and characterization of their antibacterial activity. Materials Letters. 2017; 209:201-206.
23. Bonde SR, Rathod DP, Ingle AP, Ade RB, Gade AR, Rai MK. Murraya koenigii – mediated synthesis of silver nanoparticles and its activity against three human pathogenic bacteria. Nanoscience Methods. 2012; 1:25-26.
24. Chinnappan S, Kandasamy S, Arumugam S, Seralathan KK, Thangaswamy S, Muthusamy G. Biomimetric synthesis of silver nanoparticles using flower extract of Banhinia purpurea and its antibacterial activity against clinical pathogens. Environmental Science and Pollution Research. 2017; https://doi.org/10.1007/s11356-017-0841-1.
25. Mallikarjun K, Narsimha G, Dillip G, Praveen B, Shreedhas B, Lakshmi S. Green synthesis of silver nanoparticles using Ocimum leaf extracts and their characterization. Digital Journal of Nanometer Biostructure. 2011; 6:181-186.
26. Mishra S, Singh HB. Biosynthesized silver nanoparticles as a narrow weapon against phytopathogens exploring their scope and potential in agriculture. Applied Microbiology and Biotechnology. 2015; 99:1097-1107.
27. Pal S, Tak YK, Song JM. Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoaprticle?. A study of the gram negative bacterium Escherichia coli. Applied Environmental Microbiolgy. 2007; 73:1712-1720.
28. Yu DG. Formation of colloidal silver nanoparticles stabilized by Na+-poly (r-glutamic acid)-silver nitrate complex via chemical reaction process. Colloid Surf B. 2007; 59:171-178.
29. Chamakura K, Perez-Ballestero R, Cuo Z, Liu BS. Comparison of bactericidal activities of silver nanoparticles with common chemical disinfectants. Colloid Surf B. 2011; 84:88-96.
30. Fayaz, AM, Balaji, K, Girital, M, Yadav, R, Kalichelvan, PT, Venkatesan, R. Biogenic synthesis of silver nanoparticles and their synthesistic effect with antibiotics: a study against gram-postivie and num-negative bacteria. Nanomedicine and Nanotechnology Bio medicine. 2010; 6:103-109.
31. Morones JR, Elechiguerra JL, Camacho A, Ramirez JT. The bactericidal effect of the silver nanoparticles. Nanotechnology. 2005; 16:2346-2353
32. Sondi I, Salopelesondi B. Silver nanoparticles antimicrobial agent: a case study on E.coli as a model for gram negative bacteria. Journal of Colloid Interface. 2007; 275:177-182
Statistics
44 Views | 55 Downloads
How to Cite
Soris, P. T., Beulah, G. G. P., Doss, A., & Mohan, V. R. (2018). Croton sparsiflorus whole plant extract mediated biosynthesis of metallic silver nanoparticles and their antibacterial activity. Journal of Drug Delivery and Therapeutics, 8(6), 91-97. https://doi.org/10.22270/jddt.v8i6.2023