Development and validation of novel HPLC method for the estimation of Rutin in crude hydromethanolic leaf extract of Prosopis cineraria

  • Ram Singh Bishnoi Research Scholar, Department of Pharmaceutical Sciences, Mohanlal Sukhadia University, Udaipur, India
  • Manish Kumar Research Scholar, Department of Pharmaceutical Sciences, Mohanlal Sukhadia University, Udaipur, India
  • Ajay Kumar Shukla Research Scholar, Department of Pharmaceutical Sciences, Mohanlal Sukhadia University, Udaipur, India
  • C.P. Jain Professor, Department of Pharmaceutical Sciences, Mohanlal Sukhadia University, Udaipur, India

Abstract

A simple, specific, accurate and precise high performance liquid chromatography method has developed for the estimation of rutin in Prosopis cineraria. The chromatographic separation was achieved by using C18 column, 150 x 4.6mm i.d., 5µ bonded phase octadecylsilane (Thermo Labs Hypersil). Mobile phase was composed of 80 parts of methanol & 20 parts of 0.05% formic acid. The pH of the mobile phase was 3.2.The retention time of rutin was found 5.7 min with 1 mL/min flow rate at ambient temperature. The estimation was performed on PDA detector at 281 nm. In this study, an excellent linearity was obtained with r2 0.999. Besides, the chromatographic peak was found sharp & symmetric. The proposed method was validated in terms of the analytical parameters such as accuracy, linearity, precision, robustness, limit of detection (LOD), limit of quantification (LOQ) were determined based on the International Conference on Harmonization (ICH) guidelines. The detector response was linear in the range of 2-10 µg/mL. The proposed method was successfully applied for the estimation of the constituents in crude extract of Prosopis cineraria. This study established a quantitative method for the determination of rutin from Prosopis cineraria.


 Keywords: Prosopis cineraria, HPLC, Validation, Rutin.

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Author Biographies

Ram Singh Bishnoi, Research Scholar, Department of Pharmaceutical Sciences, Mohanlal Sukhadia University, Udaipur, India

Research Scholar, Department of Pharmaceutical Sciences, Mohanlal Sukhadia University, Udaipur, India

Manish Kumar, Research Scholar, Department of Pharmaceutical Sciences, Mohanlal Sukhadia University, Udaipur, India

Research Scholar, Department of Pharmaceutical Sciences, Mohanlal Sukhadia University, Udaipur, India

Ajay Kumar Shukla, Research Scholar, Department of Pharmaceutical Sciences, Mohanlal Sukhadia University, Udaipur, India

Research Scholar, Department of Pharmaceutical Sciences, Mohanlal Sukhadia University, Udaipur, India

C.P. Jain, Professor, Department of Pharmaceutical Sciences, Mohanlal Sukhadia University, Udaipur, India

Professor, Department of Pharmaceutical Sciences, Mohanlal Sukhadia University, Udaipur, India

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How to Cite
Bishnoi, R. S., Kumar, M., Shukla, A. K., & Jain, C. (2018). Development and validation of novel HPLC method for the estimation of Rutin in crude hydromethanolic leaf extract of Prosopis cineraria. Journal of Drug Delivery and Therapeutics, 8(6), 68-73. https://doi.org/10.22270/jddt.v8i6.2016