Development and validation of novel HPLC method for the estimation of Rutin in crude hydromethanolic leaf extract of Prosopis cineraria
A simple, specific, accurate and precise high performance liquid chromatography method has developed for the estimation of rutin in Prosopis cineraria. The chromatographic separation was achieved by using C18 column, 150 x 4.6mm i.d., 5µ bonded phase octadecylsilane (Thermo Labs Hypersil). Mobile phase was composed of 80 parts of methanol & 20 parts of 0.05% formic acid. The pH of the mobile phase was 3.2.The retention time of rutin was found 5.7 min with 1 mL/min flow rate at ambient temperature. The estimation was performed on PDA detector at 281 nm. In this study, an excellent linearity was obtained with r2 0.999. Besides, the chromatographic peak was found sharp & symmetric. The proposed method was validated in terms of the analytical parameters such as accuracy, linearity, precision, robustness, limit of detection (LOD), limit of quantification (LOQ) were determined based on the International Conference on Harmonization (ICH) guidelines. The detector response was linear in the range of 2-10 µg/mL. The proposed method was successfully applied for the estimation of the constituents in crude extract of Prosopis cineraria. This study established a quantitative method for the determination of rutin from Prosopis cineraria.
Keywords: Prosopis cineraria, HPLC, Validation, Rutin.
2. Anand S, Thakur S, Gargi M, Choudhary S, Bhardwaj P, Development and characterization of genomic microsatellite markers in Prosopis cineraria. Current Plant Biology, 2017; 9-10:37-42. doi: 10.1016/j.cpb.2017.03.001
3. Garg A, Mittal S, Review on Prosopis cineraria: A potential herb of Thar desert. Drug Invention Today, 2013; 5(1):60-65. doi: 10.1016/j.dit.2013.03.002
4. Ross J, Kasum C, Dietary Flavonoids: Bioavailability, Metabolic Effects, and Safety. Annual Review of Nutrition, 2002; 22(1):19-34. doi: 10.1146/annurev.nutr.22.111401.144957
5. Cody V, ChemInform Abstract: Crystal and Molecular Structures of Flavonoids. Cheminform, 1989; 20(12). doi: 10.1002/chin.198912363
6. Cao J, Zhang Y, Chen W, Zhao X, The relationship between fasting plasma concentrations of selected flavonoids and their ordinary dietary intake. British Journal Of Nutrition, 2009; 103(02):249. doi: 10.1017/s000711450999170x
7. Hakkinen S, Kärenlampi S, Heinonen I, Mykkanen H, Torronen A, Content of the Flavonols Quercetin, Myricetin, and Kaempferol in 25 Edible Berries. Journal of Agricultural And Food Chemistry, 1999; 47(6):2274-2279. doi: 10.1021/jf9811065
8. Scholz, Williamson, Interactions Affecting the Bioavailability of Dietary Polyphenols in Vivo. International Journal For Vitamin And Nutrition Research, 2007; 77(3):224-235. doi: 10.1024/0300-9822.214.171.124
9. Manach C, Williamson G, Morand C, Scalbert A, Rémésy C, Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. The American Journal Of Clinical Nutrition, 2005; 81(1):230S-242S. doi: 10.1093/ajcn/81.1.230s
10. Shah W, Rane N, Kekare MB, Vaidya V, Estimation of two bioactive compounds from Azadiracta indica A.Juss. leaves using HPLC. International Journal of Pharma and Bio Sciences; 2010; 1(2):1-7.
11. Behnaz M, Davood E A, Atena A, Simultaneous determination of rutin and quercetin in different parts of Capparis spinosa, Bulletin of environment, Pharmacology and Life Sciences 2013; 2(2):35-38.
12. Crozier, A, Jensen E, Lean M, McDonald M, Quantitative analysis of flavonoids by reversed-phase high-performance liquid chromatography. Journal Of Chromatography A, 1997; 761(1-2):315-321. doi: 10.1016/s0021-9673(96)00826-6
13. Wang S, Huang K, Determination of flavonoids by high-performance liquid chromatography and capillary electrophoresis. Journal of Chromatography A, 2004; 1032(1-2):273-279. doi: 10.1016/j.chroma.2003.11.099
14. Ashok Kumar BS, Lakshman K, Jayaveera KN, Vamshi Krishna N, Manjunath M, Suresh MV, Estimation of rutin and quercetin in Amaranthus viridis Linn by HPLC, Asian Journal of Experimental Sciences, 2009; 23:51-54.
15. ICH, Q2, Text on validation of Analytical procedures: Methodology, International conference on Harmonization, 2005.
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