Technology Overview and Current Biomedical Application of Polymeric Nanoparticles

  • Gurpreet Singh Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar Punjab-143005,
  • Abdul Faruk Head, Department of Pharmaceutical Sciences, HNB Garhwal University (A Central University) Chauras Campus, P.O. Kilkeleshwar, Via Kirtinagar Distt. Tehri Garhwal-249161 Uttrakhand
  • Preet Mohinder Singh Bedi Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005, India


Polymeric nanoparticle are of great importance in the treatment of various diseases, due to the flexibility in the  modification of their structures. Recent advances in the field of nanotechnology facilitate the engineering of multifunctional polymeric nanoparticles. All the scientific efforts of the pharmaceuticals companies are mainly focusing on two basic aspects, one is to discover new molecules of potential therapeutic interest and second is to develop of a new drug delivery system. In the last few decades,  research and development (R&D) scientists has directed their efforts toward formulating novel drug delivery systems that includes sustained and controlled release, modified release and targeted drug release dosage forms. Application of nanoscience and nanotechnology has opened several new possibilities in development of formulation This review compiles the different preparation methods of polymeric nanoparticles and then briefly explained their current potential applications.

Keywords: Polymeric nanoparticles, PLGA, Biomedical applications, Biodegradable, Dialysis method


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Author Biographies

Gurpreet Singh, Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar Punjab-143005,

Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar Punjab-143005,

Abdul Faruk, Head, Department of Pharmaceutical Sciences, HNB Garhwal University (A Central University) Chauras Campus, P.O. Kilkeleshwar, Via Kirtinagar Distt. Tehri Garhwal-249161 Uttrakhand

Head,  Department of Pharmaceutical Sciences, HNB Garhwal University (A Central University) Chauras Campus, P.O. Kilkeleshwar, Via Kirtinagar Distt. Tehri Garhwal-249161 Uttrakhand

Preet Mohinder Singh Bedi, Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005, India

Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005, India


1. Alfarouk KO, Stock CM, Taylor S, Walsh M, Muddathir AK, Verduzco D, et al. Resistance to cancer chemotherapy: failure in drug response from ADME to P-gp. Cancer cell international. 2015;15:71. PubMed PMID: 26180516. Pubmed Central PMCID: 4502609.
2. Jabir NR, Tabrez S, Ashraf GM, Shakil S, Damanhouri GA, Kamal MA. Nanotechnology-based approaches in anticancer research. International journal of nanomedicine. 2012;7:4391-408. PubMed PMID: 22927757. Pubmed Central PMCID: 3420598.
3. Yashwant P, Deepak T. Drug delivery nanoparticles formulation and characterization, Drugs and Pharmaceutical Science Series. Informa healthcare USA, 191 (2009) 1-30.
4. Manallack DT, Prankerd RJ, Yuriev E, Oprea TI, Chalmers DK. The significance of acid/base properties in drug discovery. Chemical Society reviews. 2013; 42(2):485-96. PubMed PMID: 23099561. Pubmed Central PMCID: 3641858
5. Buzea C, Pacheco, II, Robbie K. Nanomaterials and nanoparticles: sources and toxicity. Biointerphases. 2007; 2(4):MR17-71. PubMed PMID: 20419892.
6. Abhilash M.. Potential applications of Nanoparticles. 1. International Journal of Pharma and Bio Sciences. 2010; 1(1):1–12.
7. Chan JM, Valencia PM, Zhang L, Langer R, Farokhzad OC. Polymeric nanoparticles for drug delivery. Methods in molecular biology. 2010; 624:163-75. PubMed PMID: 20217595.
8. Muller RH, Mader K, Gohla S. Solid lipid nanoparticles (SLN) for controlled drug delivery - a review of the state of the art. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik eV. 2000; 50(1):161-77. PubMed PMID: 10840199.
9. Cheng CM, Wu KC. Nanomaterials and nanofabrication for biomedical applications. Science and technology of advanced materials. 2013; 14(4):040301. PubMed PMID: 27877583. Pubmed Central PMCID: 5090309.
10. Sahoo SK, Labhasetwar V. Nanotech approaches to drug delivery and imaging. Drug discovery today. 2003; 8(24):1112-20. PubMed PMID: 14678737.
11. Parveen S, Sahoo SK. Polymeric nanoparticles for cancer therapy. Journal of drug targeting. 2008; 16(2):108-23. PubMed PMID: 18274932.
12. Letchford K, Burt H. A review of the formation and classification of amphiphilic block copolymer nanoparticulate structures: micelles, nanospheres, nanocapsules and polymersomes. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik eV. 2007; 65(3):259-69. PubMed PMID: 17196803.
13. Diaspro A, Krol S, Cavalleri O, Silvano D, Gliozzi A. Microscopical characterization of nanocapsules templated on ionic crystals and biological cells toward biomedical applications. IEEE transactions on nanobioscience. 2002; 1(3):110-5. PubMed PMID: 16696300.
14. Kothamasu P, Kanumur H, Ravur N, Maddu C, Parasuramrajam R, Thangavel S. Nanocapsules: the weapons for novel drug delivery systems. BioImpacts : BI. 2012;2(2):71-81. PubMed PMID: 23678444.
15. Singh G, Kaur T, Kaur R, Kaur A. (2014). Recent biomedical applications and patents on biodegradable polymer-. PLGA. International Journal of Pharmacology and Pharmaceutical Sciences. (2014) 1:30-42.
16. Jeong B, Gutowska A. Lessons from nature: stimuli-responsive polymers and their biomedical applications. Trends in biotechnology. 2002; 20(7):305-11. PubMed PMID: 12062976.
17. Gustafson HH, Holt-Casper D, Grainger DW, Ghandehari H. Nanoparticle Uptake: The Phagocyte Problem. Nano today. 2015 Aug;10(4):487-510. PubMed PMID: 26640510. Pubmed Central PMCID: 4666556.
18. Gunaseelan S, Gunaseelan K, Deshmukh M, Zhang X, Sinko PJ. Surface modifications of nanocarriers for effective intracellular delivery of anti-HIV drugs. Advanced drug delivery reviews. 2010; 62(4-5):518-31. PubMed PMID: 19941919. Pubmed Central PMCID: 2841563.
19. Dizaj SM, Jafari S, Khosroushahi AY. A sight on the current nanoparticle-based gene delivery vectors. Nanoscale research letters. 2014; 9(1):252. PubMed PMID: 24936161. Pubmed Central PMCID: 4046008.
20. Ulery BD, Nair LS, Laurencin CT. Biomedical Applications of Biodegradable Polymers. Journal of polymer science Part B, Polymer physics. 2011; 49(12):832-64. PubMed PMID: 21769165. Pubmed Central PMCID: 3136871.
21. Vilar G, Tulla-Puche J, Albericio F. Polymers and drug delivery systems. Current drug delivery. 2012; 9(4):367-94. PubMed PMID: 22640038.
22. Gross RA, Kalra B. Biodegradable polymers for the environment. Science. 2002; 297(5582):803-7. PubMed PMID: 12161646.
23. Li X, Jasti B. Design of Controlled Release Drug Delivery Systems. New York: McGrawHill, 2006. P. 271-304.
24. Mudshinge SR, Deore AB, Patil S, Bhalgat CM. Nanoparticles: Emerging carriers for drug delivery. Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society. 2011; 19(3):129-41. PubMed PMID: 23960751. Pubmed Central PMCID: 3744999.
25. El-Say KM, El-Sawy HS. Polymeric nanoparticles: Promising platform for drug delivery. International journal of pharmaceutics. 2017; 528(1-2):675-91. PubMed PMID: 28629982.
26. Wang XQ, Zhang Q. pH-sensitive polymeric nanoparticles to improve oral bioavailability of peptide/protein drugs and poorly water-soluble drugs. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik eV. 2012; 82(2):219-29. PubMed PMID: 22885229.
27. Yang HW, Hua MY, Liu HL, Huang CY, Wei KC. Potential of magnetic nanoparticles for targeted drug delivery. Nanotechnology, science and applications. 2012; 5:73-86. PubMed PMID: 24198498. Pubmed Central PMCID: 3781723.
28. Hotze EM, Phenrat T, Lowry GV. Nanoparticle aggregation: challenges to understanding transport and reactivity in the environment. Journal of environmental quality. 2010; 39(6):1909-24. PubMed PMID: 21284288
29. De Jong WH, Borm PJ. Drug delivery and nanoparticles:applications and hazards. International journal of nanomedicine. 2008; 3(2):133-49. PubMed PMID: 18686775. Pubmed Central PMCID: 2527668.
30. Paliwal R, Babu RJ, Palakurthi S. Nanomedicine scale-up technologies: feasibilities and challenges. AAPS PharmSciTech. 201; 15(6):1527-34. PubMed PMID: 25047256. Pubmed Central PMCID: 4245446.
31. Barthel AK, Dass M, Droge M, Cramer JM, Baumann D, Urban M, et al. Imaging the intracellular degradation of biodegradable polymer nanoparticles. Beilstein journal of nanotechnology. 2014; 5:1905-17. PubMed PMID: 25383302. Pubmed Central PMCID: 4222285.
32. Wu L, Zhang J, Watanabe W. Physical and chemical stability of drug nanoparticles. Advanced drug delivery reviews. 2011; 63(6):456-69. PubMed PMID: 21315781.
33. Frohlich E. The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles. International journal of nanomedicine. 2012; 7:5577-91. PubMed PMID: 23144561. Pubmed Central PMCID: 3493258.
34. Li RY, Liu ZG, Liu HQ, Chen L, Liu JF, Pan YH. Evaluation of biocompatibility and toxicity of biodegradable poly (DL-lactic acid) films. American journal of translational research. 2015; 7(8):1357-70. PubMed PMID: 26396667. Pubmed Central PMCID: 4568792.
35. Wallace SJ, Li J, Nation RL, Boyd BJ. Drug release from nanomedicines: Selection of appropriate encapsulation and release methodology. Drug delivery and translational research. 2012; 2(4):284-92. PubMed PMID: 23110256. Pubmed Central PMCID: 3482165.
36. Quintanar-Guerrero D, Allemann E, Fessi H, Doelker E. Preparation techniques and mechanisms of formation of biodegradable nanoparticles from preformed polymers. Drug development and industrial pharmacy. 1998; 24(12):1113-28. PubMed PMID: 9876569.
37. Takeuchi H, Yamamoto H, Kawashima Y. Mucoadhesive nanoparticulate systems for peptide drug delivery. Advanced drug delivery reviews. 2001; 47(1):39-54. PubMed PMID: 11251244.
38. Song CX, Labhasetwar V, Murphy H, Qu X, Humphrey WR, Shebuski RJ, et al. Formulation and characterization of biodegradable nanoparticles for intravascular local drug delivery. Journal of Controlled Release. 1997; 43(2):197-212.
39. Alex R, Bodmeier R. Encapsulation of water-soluble drugs by a modified solvent evaporation method. I. Effect of process and formulation variables on drug entrapment. Journal of microencapsulation. 1990; 7(3):347-55. PubMed PMID: 2384837.
40. Obeidat WM, Price JC. Viscosity of polymer solution phase and other factors controlling the dissolution of theophylline microspheres prepared by the emulsion solvent evaporation method. Journal of microencapsulation. 2003; 20(1):57-65. PubMed PMID: 12519702.
41. Reis CP, Neufeld RJ, Ribeiro AJ, Veiga F. Nanoencapsulation I. Methods for preparation of drug-loaded polymeric nanoparticles. Nanomedicine. 2006; 2(1):8-21. PubMed PMID: 17292111
42. Jaiswal J, Gupta SK, Kreuter J. Preparation of biodegradable cyclosporine nanoparticles by high-pressure emulsification-solvent evaporation process. Journal of controlled release : official journal of the Controlled Release Society. 2004 Apr 16;96(1):169-78. PubMed PMID: 15063039.
43. Soppimath, K. S., Aminabhavi, T. M., Kulkarni, A. R., & Rudzinski, W. E. (2001). Biodegradable polymeric nanoparticles as drug delivery devices. J Control Release, 70(1-2), 1-20.
44. Tice TR, Gilley RM. Preparation of injectable controlled-release microcapsules by a solvent-evaporation process. Journal of Controlled Release. 1985; 2:343-52.
45. Lemarchand C, Gref R, Passirani C, Garcion E, Petri B, Muller R, et al. Influence of polysaccharide coating on the interactions of nanoparticles with biological systems. Biomaterials. 2006; 27(1):108-18. PubMed PMID: 16118015.
46. Sjostrom B, Kaplun A, Talmon Y, Cabane B. Structures of nanoparticles prepared from oil-in-water emulsions. Pharmaceutical research. 1995; 12(1):39-48. PubMed PMID: 7724486.
47. Shunmugaperumal T, Sudalaimuthu Ramachandran S, Raj B, Thenrajan RS. Manufacturing techniques and excipients used during the formulation of oil-in-water type nanosized emulsions for medical applications. Journal of Excipients and Food Chemicals. 2010; 1(1):11-29.
48. Ficheux MF, Bonakdar L, Leal-Calderon F, Bibette J. Some Stability Criteria for Double Emulsions. Langmuir : the ACS journal of surfaces and colloids. 1998; 14(10):2702-6.
49. McCall RL, Sirianni RW. PLGA nanoparticles formed by single- or double-emulsion with vitamin E-TPGS. Journal of visualized experiments : JoVE. 2013; 27(82):51015. PubMed PMID: 24429733. Pubmed Central PMCID: 4106449.
50. Fessi H, Puisieux F, Devissaguet JP, Ammoury N, Benita S. Nanocapsule formation by interfacial polymer deposition following solvent displacement. International journal of pharmaceutics. 1989; 55(1):R1-R4.
51. Barichello JM, Morishita M, Takayama K, Nagai T. Encapsulation of hydrophilic and lipophilic drugs in PLGA nanoparticles by the nanoprecipitation method. Drug development and industrial pharmacy. 1999; 25(4):471-6. PubMed PMID: 10194602.
52. Chorny M, Fishbein I, Danenberg HD, Golomb G. Lipophilic drug loaded nanospheres prepared by nanoprecipitation: effect of formulation variables on size, drug recovery and release kinetics. Journal of controlled release : official journal of the Controlled Release Society. 2002; 83(3):389-400. PubMed PMID: 12387947.
53. Galindo-Rodriguez S, Allemann E, Fessi H, Doelker E. Physicochemical parameters associated with nanoparticle formation in the salting-out, emulsification-diffusion, and nanoprecipitation methods. Pharmaceutical research. 2004; 21(8):1428-39. PubMed PMID: 15359578.
54. Nah JW, Paek YW, Jeong YI, Kim DW, Cho CS, Kim SH, et al. Clonazepam release from poly(DL-lactide-co-glycolide) nanoparticles prepared by dialysis method. Archives of pharmacal research. 1998; 21(4):418-22. PubMed PMID: 9875469
55. Ekman B, Sjöholm I. Improved Stability of Proteins Immobilized in Microparticles Prepared by a Modified Emulsion Polymerization Technique. Journal of pharmaceutical sciences. 1978; 67(5):693-6.
56. Lowe PJ, Temple CS. Calcitonin and insulin in isobutylcyanoacrylate nanocapsules: protection against proteases and effect on intestinal absorption in rats. The Journal of pharmacy and pharmacology. 1994; 46(7):547-52. PubMed PMID: 7996380.
57. Crespy D, Landfester K. Miniemulsion polymerization as a versatile tool for the synthesis of functionalized polymers. Beilstein journal of organic chemistry. 2010 Dec 1;6:1132-48. PubMed PMID: 21160567. Pubmed Central PMCID: 3002022.
58. Landfester K. Miniemulsion polymerization and the structure of polymer and hybrid nanoparticles. Angewandte Chemie. 2009; 48(25):4488-507. PubMed PMID: 19455531.
59. Ibrahim H, Bindschaedler C, Doelker E, Buri P, Gurny R. Aqueous nanodispersions prepared by a salting-out process. International journal of pharmaceutics. 1992; 87(1):239-46.
60. Rao JP, Geckeler KE. Polymer nanoparticles: Preparation techniques and size-control parameters. Progress in Polymer Science. 2011; 36(7):887-913.
61. Allemann E, Leroux JC, Gurny R, Doelker E. In vitro extended-release properties of drug-loaded poly(DL-lactic acid) nanoparticles produced by a salting-out procedure. Pharmaceutical research. 1993; 10(12):1732-7. PubMed PMID: 7905625.
62. Calvo P, Remuñán-López C, Vila-Jato JL, Alonso MJ. Novel hydrophilic chitosan-polyethylene oxide nanoparticles as protein carriers. Journal of Applied Polymer Science. 1997; 63(1):125-32.
63. Grenha A. Chitosan nanoparticles: a survey of preparation methods. Journal of drug targeting. 2012; 20(4):291-300. PubMed PMID: 22296336.
64. Nasti A, Zaki NM, de Leonardis P, Ungphaiboon S, Sansongsak P, Rimoli MG, et al. Chitosan/TPP and chitosan/TPP-hyaluronic acid nanoparticles: systematic optimisation of the preparative process and preliminary biological evaluation. Pharmaceutical research. 2009; 26(8):1918-30. PubMed PMID: 19507009.
65. Patel BB, Patel JK, Chakraborty S. Review of patents and application of spray drying in pharmaceutical, food and flavor industry. Recent patents on drug delivery & formulation. 2014; 8(1):63-78. PubMed PMID: 24720661.
66. Esposito E, Cervellati F, Menegatti E, Nastruzzi C, Cortesi R. Spray dried Eudragit microparticles as encapsulation devices for vitamin C. International journal of pharmaceutics. 2002; 242(1-2):329-34. PubMed PMID: 12176273.
67. Sinsuebpol C, Chatchawalsaisin J, Kulvanich P. Preparation and in vivo absorption evaluation of spray dried powders containing salmon calcitonin loaded chitosan nanoparticles for pulmonary delivery. Drug design, development and therapy. 2013; 7:861-73. PubMed PMID: 24039397. Pubmed Central PMCID: 3770519.
68. Gallo L, Bucala V. A Review on Influence of Spray Drying Process Parameters on the Production of Medicinal Plant Powders. Current drug discovery technologies. 2018. E-pub PubMed PMID: 30068280.
69. Deshpande PB, Kumar GA, Kumar AR, Shavi GV, Karthik A, Reddy MS, et al. Supercritical fluid technology: concepts and pharmaceutical applications. PDA journal of pharmaceutical science and technology. 2011; 65(3):333-44. PubMed PMID: 22293238.
70. Kalani M, Yunus R. Application of supercritical antisolvent method in drug encapsulation: a review. International journal of nanomedicine. 2011; 6:1429-42. PubMed PMID: 21796245. Pubmed Central PMCID: 3141870.
71. Debenedetti PG, Tom JW, Kwauk X, Yeo SD. Rapid expansion of supercritical solutions (ress ): fundamentals and applications. Fluid Phase Equilibria. 1993; 82:311-21.
72. Verma D, Thakur PS, Padhi S, Khuroo T, Talegaonkar S, Iqbal Z. Design expert assisted nanoformulation design for co-delivery of topotecan and thymoquinone: Optimization, in vitro characterization and stability assessment. Journal of Molecular Liquids. 2017; 242(Supplement C):382-94.
73. Ravikumara NR, Bharadwaj M, Madhusudhan B. Tamoxifen citrate-loaded poly(d,l) lactic acid nanoparticles: Evaluation for their anticancer activity in vitro and in vivo. Journal of biomaterials applications. 2016; 31(5):755-72. PubMed PMID: 27664187. Epub 2016/10/30. eng.
74. Khare V, Singh A, Mahajan G, Alam N, Kour S, Gupta M, et al. Long-circulatory nanoparticles for gemcitabine delivery: Development and investigation of pharmacokinetics and in-vivo anticancer efficacy. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. 2016; 92:183-93. PubMed PMID: 27404580. Epub 2016/07/13. eng.
75. Katiyar SS, Muntimadugu E, Rafeeqi TA, Domb AJ, Khan W. Co-delivery of rapamycin- and piperine-loaded polymeric nanoparticles for breast cancer treatment. Drug delivery. 2016; 23(7):2608-16. PubMed PMID: 26036652. Epub 2016/10/18. eng.
76. Pandey SK, Patel DK, Maurya AK, Thakur R, Mishra DP, Vinayak M, et al. Controlled release of drug and better bioavailability using poly(lactic acid-co-glycolic acid) nanoparticles. International journal of biological macromolecules. 2016; 89:99-110.
77. Yang A, Liu Z, Yan B, Zhou M, Xiong X. Preparation of camptothecin-loaded targeting nanoparticles and their antitumor effects on hepatocellular carcinoma cell line H22. Drug delivery. 2016; 23(5):1699-706. PubMed PMID: 25148540.
78. Cho HJ, Park JH, Kim DD, Yoon IS. Poly(lactic-co-glycolic) Acid/Solutol HS15-Based Nanoparticles for Docetaxel Delivery. Journal of nanoscience and nanotechnology. 2016; 16(2):1433-6. PubMed PMID: 27433600.
79. Chen H, Xie LQ, Qin J, Jia Y, Cai X, Nan W, et al. Surface modification of PLGA nanoparticles with biotinylated chitosan for the sustained in vitro release and the enhanced cytotoxicity of epirubicin. Colloids and surfaces B, Biointerfaces. 2016; 138:1-9. PubMed PMID: 26638176.
80. Chaurasia S, Chaubey P, Patel RR, Kumar N, Mishra B. Curcumin-polymeric nanoparticles against colon-26 tumor-bearing mice: cytotoxicity, pharmacokinetic and anticancer efficacy studies. Drug development and industrial pharmacy. 2016; 42(5):694-700. PubMed PMID: 26165247.
81. Yuan Z, Qu X, Wang Y, Zhang DY, Luo JC, Jia N, et al. RETRACTED: Enhanced antitumor efficacy of 5-fluorouracil loaded methoxy poly(ethylene glycol)-poly(lactide) nanoparticles for efficient therapy against breast cancer. Colloids and surfaces B, Biointerfaces. 2015 Apr 01;128:489-97. PubMed PMID: 25779606.
82. Ray S, Mishra A, Mandal TK, Sa B, Chakraborty J. Optimization of the process parameters for the fabrication of a polymer coated layered double hydroxide-methotrexate nanohybrid for the possible treatment of osteosarcoma. RSC Advances. 2015; 5(124):102574-92.
83. 16. Yu CY, Wang YM, Li NM, Liu GS, Yang S, Tang GT, et al. In vitro and in vivo evaluation of pectin-based nanoparticles for hepatocellular carcinoma drug chemotherapy. Molecular pharmaceutics. 2014; 11(2):638-44. PubMed PMID: 24383625.
84. Sadhukha T, Prabha S. Encapsulation in nanoparticles improves anti-cancer efficacy of carboplatin. AAPS PharmSciTech. 2014; 15(4):1029-38. PubMed PMID: 24831091. Pubmed Central PMCID: PMC4113618.
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Singh G, Faruk A, Bedi PMS. Technology Overview and Current Biomedical Application of Polymeric Nanoparticles. JDDT [Internet]. 15Nov.2018 [cited 16May2022];8(6):285-9. Available from: