Biochemical characterization and 16s rRNA sequencing of different bacteria from textile dye effluents
Environmental pollution has been identified as a major problem in the modern world. Dyeing effluents have become a vital source of water pollution. Release of coloured textile effluents is undesirable in the aquatic environment as they reduce light penetration, thereby affecting aquatic life and limits utilization of the water media. In Tirupur, the textile factories discharge millions of litres of untreated effluents into the drains that eventually empty into river, Noyyal. The release of coloured compound into water bodies is undesirable not only because of their impact on photosynthesis of aquatic plants but also due to the carcinogenic nature of these dyes and their breakdown products. The ability of bacterial strains isolated from the dye effluent of textile mill sites. Morphological and biochemical characterization was done to identify isolates and was found to be Pseudomonas spp, Bacillus spp and Serattia spp. The isolated strains were finally identified by 16S rRNA sequence analysis. Bacteria are generally identified by 16S rRNA sequencing. The rRNA is the most conserved (least variable) gene in all cells. They were identified as Pseudomonas aeruginosa, Bacillus amyloliquefaciens and Serattia liquefaciens. The sequences were deposited in GENBANK. The accession numbers were KU041528, KU041530 and KU041531 respectively. The identification was conformed by 16S rRNA sequencing.
Keywords: Textile Dye Effluents, Bacteria, 16S rRNA, NCBI.
2. Nigam P, Banat IM, Singh D, Marchant R. Biochem, 1996; 31:435-442.
3. Carliell CM, Barclay SJ, Naidoo N, Buckley CA, Mulholland DA, Senior E. Water SA. 1995; 2161.
4. Golka K, Kopps S, Myslak ZW. Toxicol. Lett., 2004; 151:203.
5. Sharma P, Singh L, Dilbaghi N. Journal of Hazardous Materials. 2009; 161:1081.
6. Pinheiro HM, Touraud E, Thomas O. Dyes Pigments. 2004; 61: 121.
7. Martiny JBH. Microbial biogeography: putting microorganisms on the map. Nat. Rev. Microbiol. 2006; 4:102–112.
8. Butler CS, Mason JR. Structure, function analysis of the bacterial aromatic ring hydroxylating dioxygenases. Adv Microb Physiol. 1997; 38: 47–84.
9. Ellis BML. Environmental biotechnology informatics. Curr Opin Biotechnol. 2000; 11:232–235.
10. Grekova-Vasileva1 M, Popov I, Vassilev D, Topalova1 Y. Isolation and characterisation of capable of azo dye decolourisation. Biotechnol. & Biotechnol. Eq. 2009; 23.
11. Kaufmann SHE, Schaible UE. 100th anniversary of Robert Koch’s Nobel Prize for the discovery of the tubercle bacillus. Trends Microbiol. 2005; 13:469–475.
12. Christensen BB, Haagensen JA, Heydorn A, Molin S. Metabolic commensalism and competition in a two-species microbial consortium. Appl. Environ. Microbiol. 2002; 68:2495–2502.
13. Lewis K. Riddle of biofilm resistance. Antimicrob. Agents Chemother. 2001; 45:999–1007.
14. Moons P, Michiels CW, Aertsen A. Bacterial interactions in biofilms. Crit. Rev. Microbiol. 2009; 35: 157–168.
15. Kloos WE, Schleifer KH. Simplified scheme for routine identification of human Staphylococcus species. J. Clin. Microbiol. 1999; 1:82-88.
16. Braga PAC, Tata A, Goncalves dos Santos V, Barreiro JR, Schwab NV, Veiga dos Santos M, Eberlin MN, Ferreira CR. Bacterial identification from the agar plate to the mass spectrometer. RSC Adv. 2013; 3:994–1008.
17. Weile J, Knabbe C. Current applications and future trends of molecular diagnostics in clinical bacteriology. Anal. Bioanal. Chem. 2009; 394:731–742.
18. Sousa AM, Machado I, Pereira MO. Phenotypic switching: an opportunity to bacteria thrive. In: Mendez-Vilas, A. (Ed.), Science Against Microbial Pathogens: Communicating Current Research and Technological Advances. 2011;
19. Newby, P. Rapid methods for enumeration and identification in microbiology. In: Baird, R.M.; Hodges, N.A.; Denver, S.P. (Eds.). Handbook of microbiological control: pharmaceuticals and medical devices. London: Taylor & Francis. 2000; 107-119.
20. Bansal AK, Meyer TE. Evolutionary analysis by whole genome comparisons. J. Bacteriol. 2002; 184(8):2260-2272.
21. Song J, Lee SC, Kang JW, BAEK HJ, SUH JW. Phylogenetic analysis of Streptomyces spp. isolated from potato scab lesions in Korea on the basis of 16S rRNA gene and 16S-23S rDNA internally transcribed spacer sequences. Int. J. Syst. Evol. Microbiol. 2004; 54(1):203-209.
22. Lane DJ, Pace B, Olsen GJ, Stahlt DA, Sogint M, Pace NR. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc. Natl. Acad. Sci. USA. 1985; 82:6955-6959.
23. Patel JB. 16S rRNA gene sequencing for bacterial pathogen identification in the clinical laboratory. Mol. Diagn. 2001; 6(4): 313-321.
24. Easter CM. Rapid microbiological methods in the pharmaceutical industry. Denver: Interpharm CRC Press. 2003; 161-177.
25. Relman DA, Falkow S. Identification of uncultured microorganisms: expanding the spectrum of characterized microbial pathogens. Infect Agents Dis. 1992; 1:245–253.
26. Clarridge JE. Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin Microbiol Rev. 2004; 3(17):840–862.
27. Krieg NR, Holt JG. Bergey’s Manual of Systematic Bacteriology. Williams & Wilkins Co., Baltimore. 1984; 1:161-172.
28. Bansal AK, Meyer TE. Evolutionary analysis by whole genome comparisons. J. Bacteriol. 2002; 184(8):2260-2272.
29. Schmalenberger A, Schwieger F, Tebee CC. Effect of primers hybridizing to different evolutionarily conserved regions of the small subunit rRNA gene in PCR based microbial community analyses and genetic profiling. Appl. Environ. Microbiol. (2001); 67:3557–3563.
30. Drummond AJ, Ashton B, Buxton S, Cheung M, Cooper A, Heled J, Kearse M,Moir R, Stones-Havas S, Sturrock S, Thierer T, Wilson A. Geneiousv. 2010; 5(1). Available from.
31. Jayaseelan T, Damodaran R, Mani P. Multiple Resistant Activities And Molecular Characterisation Of Pseudomonas aeruginosa Isolated From Tirupur Textile Effluents. Canadian Journal of Biological and Microbiology Research. 2015; 1(1):18-24.
32. Mohammed EL Amine Bendaha, Boumedien Meddah, Hadj Ahmed Belaouni and Aicha Tirtouil. Isolation and biosurfactants production by Pseudomonas aeruginosa S7PS5. Journal of Chemical and Pharmaceutical Research, 2015; 7(10):413-422.
33. Singh S, Vijayanand S, Goya A. Isolation, Identification, and Characterization of a Cellulolytic Bacillus amyloliquefaciens Strain SS35 from Rhinoceros Dung ISRN Microbiol. 2013; 2013:728134.
34. Shine Kadaikunnan, Thankappan Sarasam Rejiniemon, Jamal M Khaled, Naiyf S Alharbi, Ramzi Mothana. In-vitro antibacterial, antifungal, antioxidant and functional properties of Bacillus amyloliquefaciens Annals of Clinical Microbiology and Antimicrobials. 2015; 14(9):1-11.
35. Fox GE, Wisotzkey JD, Jurtshuk P Jr. How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity. Int J Syst Bacteriol. 1992; 42(1):166-170.
36. Ai-hua Liu, Min Shi, Cai-jun Zhang, Xiao-jie Li, Xin Wang, Pei-qing Shen, Ma-lin Li, Fukai Bao and Ma-lin Li. Isolation and molecular identification of a Serratia strain from domesticated tree shrew (Tupaia belangeri) skin infectious site in Yunnan, China. African Journal of Biotechnology. 5 April, 2010; 9(14):2165-2168.
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