In-vitro antimicrobial and antioxidant activity of Argyreia cuneata (Willd.) Ker Gawl. (Convolvulaceae)
Objectives: Argyreia cuneata (Willd.) Ker Gawl. belongs to the family Convolvulaceae. The present study was performed to screen the potential of crude extract of various parts of A. cuneata to exhibit antimicrobial activity. Methods: Extraction of shade dried and powdered leaf, stem and flower of A. cuneata was carried out by maceration technique. Antibacterial and antifungal activity of extracts was evaluated by Agar well diffusion and Poisoned food technique respectively. Antioxidant activity was determined by DPPH radical scavenging, ABTS radical scavenging and ferric reducing assays. Results: All extracts were effective in inhibiting test bacteria and the susceptibility of bacteria to extracts was in the order: Bacillus cereus > Shigella flexneri > Escherichia coli > Salmonella typhimurium. Leaf extract and stem extract exhibited highest and least antibacterial activity, respectively. Extracts were effective in causing inhibition of seed-borne fungi viz. Aspergillus niger and Bipolaris sp to >50%. Leaf extract exhibited marked antifungal activity followed by flower extract and stem extract. All extracts were shown to exhibit concentration dependent scavenging and reducing activity. Antioxidant activity of extracts observed was in the order: leaf extract > flower extract > stem extract. Conclusion: Among various parts of A. cuneata, leaf extract exhibited marked antimicrobial and antioxidant activity. The plant can be employed as an effective antimicrobial and antioxidant agent in suitable form. Further studies may be undertaken to recover phytochemicals from the plant and to investigate the antimicrobial and antioxidant activity of isolated components.
Keywords: Argyreia cuneata, Maceration, Antimicrobial, Agar well diffusion, Poisoned food technique, Antioxidant
2. Gamble JS. Flora of the Presidency of Madras. Volume II. Bishen Singh Mahendra Pal Singh, Dehra Dun, India, 1993, Pp 909.
4. Semwal DK, Bamola A, Rawat U. Chemical constituents of some antidiabetic plants. Universities’ Journal of Phyto-chemistry and Ayurvedic Heights 2007; 2: 40-48.
5. Quattrocchi U. CRC world dictionary of medicinal and poisonous plants. CRC press, Boka Raton, 2012, Pp 376.
6. Kumar S, Saini M, Kumar V, Prakash O, Arya R, Rana M, Kumar D. Traditional medicinal plants curing diabetes: A promise for today and tomorrow. Asian J Tradit Med 2012; 7(4): 178-188.
7. Lingaraju DP, Sudarshana MS, Rajashekar N. Ethnopharmacological survey of traditional medicinal plants in tribal areas of Kodagu district, Karnataka, India. J Pharm Res 2013; 6(2): 284-297.
8. Dhivya SM, Kalaichelvi K. Medicinal plants used by Irula tribes of Nellithurai Beat, Karamadai Range, Western Ghats, Tamil Nadu, India: An ethnobotanical survey. Journal of Medicinal Plants Studies 2016; 4(4): 270-277.
9. Kumar PGM, Shiddamallayya N. Survey of wild medicinal plants of Hassan district, Karnataka. Journal of Medicinal Plants Studies 2016; 4(1): 91-102.
10. Raveesha HR, Sudhama VN. Ethnoveterinary practices in Mallenahalli of Chikmagalur taluk, Karnataka. Journal of Medicinal Plants Studies 2015; 3(1): 37-41.
11. Biradar SM, Rangani AT, Kulkarni VH, Joshi H, Habbu PV, Smita DM. Prevention of onset of hyperglycemia by extracts of Argyriea cuneata on alloxan-induced diabetic rats. J Pharm Res 2010; 3(9): 2186-2187.
12. Malathi S, Balasubramaniam V, Kumar JS, Kumar NR, Kaffoor AH. Antidiabetic effect of methanolic leaf extract of Argyreia cuneata Willd. Ex Ker- Awl. (Convolvulaceae). World J Pharm Pharm Sci 2017; 6(8): 1650-1663.
13. Malathi S, Balasubramaniam V, Kumar NR, Kumar JS. Evaluation of analgesic activity of methanolic leaf extract of Argyreia cuneata Willd. (Convolvulaceae). Int J Pharm Biol Sci 2017; 7(4): 231-234.
14. Manda RM, Seru G, Bakshi V. Evaluation of anti-pyretic and wound healing activities of Argyreia cuneata in Wistar Albino rats. Int J Pharm Biol Sci 2016; 6(2): 111-113.
15. Raghavendra HL, Kekuda PTR, Akarsh S, Ranjitha MC, Ashwini HS. Phytochemical analysis, antimicrobial and antioxidant activities of different parts of Pleocaulus sessilis (Nees) Bremek (Acanthaceae). Int J Green Pharm 2017; 11(2): 98-107.
16. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999; 12(4):564-582.
17. Rios JC, Recio MC. Medicinal plants and antimicrobial activity. J Ethnopharmacol 2005; 100(1-2): 80-84.
18. Raj KM, Balachandran C, Duraipandiyan V, Agastian P, Ignacimuthu S. Antimicrobial activity of Ulopterol isolated from Toddalia asiatica (L.) Lam.: A traditional medicinal plant. J Ethnopharmacol 2012; 140(1): 161-165.
19. Radulovic NS, Blagojevic PD, Stojanovic-Radic ZZ, Stojanovic NM. Antimicrobial plant metabolites: Structural diversity and mechanism of action. Curr Med Chem 2013; 20: 932-952.
20. Compean KL, Ynalvez RA. Antimicrobial activity of plant secondary metabolites: A review. Research Journal of Medicinal Plants 2014; 8: 204-213.
21. Wikaningtyas P, Sukandar EY. The antibacterial activity of selected plants towards resistant bacteria isolated from clinical specimens. Asian Pac J Trop Biomed 2016; 6(1): 16-19.
22. Long S, Li C, Hu J, Zhao Q, Chen D. Indole alkaloids from the aerial parts of Kopsia fruticosa and their cytotoxic, antimicrobial and antifungal activities. Fitoterapia 2018; 129: 145-149.
23. Rahman AM, Ahsan T, Islam S. Antibacterial and antifungal properties of the methanol extract from the stem of Argyreia argentea. Bangladesh J Pharmacol 2010; 5: 41-44.
24. Packialakshmi N, Beevi HF. Antibacterial screening on leaves of Argyreia cymosa Roxb. against pathogenic bacteria isolated from infected pateints samples wound, sputum and stool. Int J Appl Sci Biotechnol 2014; 2(3): 279-282.
25. Habbu PV, Mahadevan KM, Shastry RA, Manjunatha H. Antimicrobial activity of flavonoid sulphates and other fractions of Argyreia speciosa (Burm.f) Boj. Indian J Exp Biol 2009; 47: 121-128.
26. Mahendra C, Manasa G, Murali M, Amruthesh KN, Sudarshana MS, Lingaraju DP. Antibacterial and antioxidant properties of Argyreia osyrensis Roth. Ann Phytomed 2016; 5(1): 110-115.
27. Quiroga EN, Sampietro AR, Vattuone MA. Screening antifungal activities of selected medicinal plants. J Ethnopharmacol 2001; 74(1): 89-96.
28. Dellavalle PD, Cabrera A, Alem D, Larranaga P, Ferreira F, Rizza MD. Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp. Chilean J Agric Res 2011; 71(2): 231-239.
29. Bhalodia NR, Shukla VJ. Antibacterial and antifungal activities from leaf extracts of Cassia fistula L.: An ethnomedicinal plant. J Adv Pharm Technol Res 2011; 2(2): 104-109.
30. Dissanayake MLMC. Inhibitory effect of selected medicinal plant extracts on phytopathogenic fungus Fusarium oxysporum (Nectriaceae) Schlecht. Emend. Snyder and Hansen. Annu Res Rev Biol 2014; 4(1): 133-142.
31. Sales MDC, Costa HB, Fernandes PMB, Ventura JA, Meira DD. Antifungal activity of plant extracts with potential to control plant pathogens in pineapple. Asian Pac J Tropical Biomed 2016; 6(1): 26-31.
32. Kekuda PTR, Raghavendra HL. Antifungal activity of Helichrysum buddleioides DC. against seed borne fungi. EC Microbiology 2017; 6(2): 54-59.
33. Shabana YM, Abdalla ME, Shahin AA, El-Sawy MM, Draz IS, Youssif AW. Efficacy of plant extracts in controlling wheat leaf rust disease caused by Puccinia triticina. Egyptian Journal of Basic and Applied Sciences 2017; 4(1): 67-73.
34. Mahule A, Rai P, Ghorpade DS, Khadabadi S. In vitro antifungal activity of ethanol fractions of Argyreia nervosa (Burm.f.) Boj. leaves. Indian J Nat Prod Resour 2012; 3(1): 48-54.
35. Samarth RM, Panwar M, Kumar M, Soni A, Kumar M, Kumar A. Evaluation of antioxidant and radical-scavenging activities of certain radioprotective plant extracts. Food Chem 2008; 106(2): 868-873.
36. Sylvie DD, Anatole PC, Cabral BP, Veronique PB. Comparison of in vitro antioxidant properties of extracts from three plants used for medical purpose in Cameroon: Acalypha racemosa, Garcinia lucida and Hymenocardia lyrata. Asian Pac J Trop Biomed 2014; 4(Suppl 2): S625-S632.
37. Pavithra K, Vadivukkarasi S. Evaluation of free radical scavenging activity of various extracts of leaves from Kedrostis foetidissima (Jacq.) Cogn. Food Science and Human Wellness 2015; 4(1): 42-46.
38. Jayathilake C, Rizliya V, Liyanage R. Antioxidant and free radical scavenging capacity of extensively used medicinal plants in Sri Lanka. Procedia Food Sci 2016; 6: 123-126.
39. Adebiyi OE, Olayemi FO, Ning-Hua T, Guang-Zhi Z. In vitro antioxidant activity, total phenolic and flavonoid contents of ethanol extract of stem and leaf of Grewia carpinifolia. Beni-Suef Univ J Basic Appl Sci 2017; 6(1): 10-14.
40. Sareedenchai V, Wiwat C, Wongsinkongman P, Soonthornchareonnon N. In vitro testing of anti-HIV and antioxidative activities of Argyreia nervosa (Burm.f) Bojor leaves. Mahidol Univ J Pharm Sci 2014; 41(4): 47-53.
41. Prashanth MK, Revanasiddappa HD, Rai KML, Raveesha KA, Jayalakshmi B2. Antibacterial, anthelmintic and antioxidant activity of Argyreia elliptica extracts: Activity enhancement by the addition of metal salts. Int J Appl Res Nat Prod 2013; 6(3): 1-10.
42. Uddin MN, Rahman MA, Mitra K, Akter R. Preliminary phytochemical analysis and in vitro antioxidant activities of methanol extract of Argyreia argentea (Roxb). Int J Appl Res Nat Prod 2014; 7(1): 1-7.
43. Baruah NC, Das S, Yadav SK. Preliminary phyto-chemical analysis and antioxidant activities of methanol extract of Argyreia roxburghii Choisy. International Journal of Herbal Medicine 2014; 2(1): 126-131.
44. Badami S, Vaijanathappa J, Bhojraj S. In vitro antioxidant activity of Argyreia cymosa bark extracts. Fitoterapia 2008; 79(4): 287-289.
45. Nishaa S, Vishnupriya M, Sasikumar jM, Christabel HP, Gopalakrishnan VK. Antioxidant activity of ethanolic extract of Maranta arundinacea .L tuberous rhizomes. Asian J Pharm Clin Res 2012; 5(4): 85-88.
46. Lu J, Fu X, Liu T, Zheng Y, Chen J, Luo F. Phenolic composition, antioxidant, antibacterial and anti-inflammatory activities of leaf and stem extracts from Cryptotaenia japonica Hassk. Ind Crops Prod 2018; 122: 522-532.
47. Joshi BB, Chudasama P, Patel J, Mistry KN. In vitro evaluation of anti-oxidant and anti-proliferative activity in crude extracts of Argyreia nervosa roots. World J Pharm Pharm Sci 2014; 3(11): 1235-1250.
48. Shanta MA, Ahmed T, Uddin MN, Majumder S, Hossain MS, Rana MS. Phytochemical screening and in vitro determination of antioxidant potential of methanolic extract of Streospermum chelonoides. J Appl Pharm Sci, 2013; 3(3):117-121.
49. Vijayalakshmi M, Ruckmani K. Ferric reducing anti-oxidant power assay in plant extract. Bangladesh J Pharmacol 2016; 11: 570-572.
50. Benslama A, Harrar A. Free radicals scavenging activity and reducing power of two Algerian Sahara medicinal plants extracts. International Journal of Herbal Medicine 2016; 4(6): 158-161.
51. Sahana BK, Akhilesha S, Priyanka GS, Kekuda PTR. Antioxidant and antifungal activity of Geophila repens (L.) I. M. Johnst. (Rubiaceae). J Drug Delivery Ther 2018; 8(5): 268-272.
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