PREPARATION AND CHARACTERIZATION OF CHITOSAN NANOPARTICLES OF INSULIN FOR NASAL DELIVERY
The aim of this study was to prepare and evaluate nanoparticles containing insulin in different polymer ratio by ionotropic gelation method. The average particle size was found to be 130.4±3.4 -155.5±6.4nm. SEM indicates that nanoparticles have shown smooth and spherical shape. The zeta potential of optimized formulation was 35.5 mv which indicates moderate stability with no agglomeration. The average drug content was found to be 130.4±3.4 -155.5±6.4nm. The in vitro drug release data was analyzed using zero order, first order, Higuchi, and Korsmeyer-Peppas models. It was observed the best fit model for nanoparticles was higuchi model. The developed formulation in situ polymeric gel is designed in such a way that the gel will load insulin in higher concentration and it will also contain penetration enhancer which will enhance the absorption of release drug from gel to systemic circulation.
Keywords: Insulin, Nanoparticle, invitro drug release study.
2. Attivi, D. Wehrle, P. Ubrich, N. Damge, C. Hoffman, M. Maincent, P. Drug. Dev. Ind. Pharm. 2005; 31(2):179.
3. Sarmento, B. Ribeiro, A. Veiga, F. Sampaio, P. Neufeld, R. Ferreira, D. Pharm. Res. 2007; 24(12):2198.
4. Bachnav D., Rao M., Madgulkar A., Rao S., Nanotechnology and blood brain barrier, Indian Drug, 2007; 44:245-252.
5. Chen D.B., Yang T. Z., Lu W. L., Zhang Q., In vitro and in vivo study of two types of long circulating solid lipid nanoparticles containing pacitaxel, Chem. Pharm. Bull., 2001; 49:1444- 1447.
6. Calvo et al, Novel Hydrophilic Chitosan-Polyethylene Oxide Nanoparticles as Protein Carriers, Journal of Applied Polymer Science 1997; 63:125-132.
7. Peltonen L, Koistinen P, Karjalainen M, Hakkinen A, Hirvonen J. The effect of cosolvents on the formulation of nanoparticles from low molecular weight poly(l)lactide, AAPS PharmSciTech, 2002; 3:1-7.
8. Cui F, Oian F, Yin C. Preparation and characterization of mucoadhesive polymercoated nanopaticles, Int. J. Pharm., 2006, 316, 154-161.
9. Mahjub R, Dorkoosh FA, Amini M, Khoshayand MR, Rafiee-tehrani M. Preparation, statistical optimization, and in vitro characterization of insulin nanoparticles composed of quaternized aromatic derivatives of chitosan, AAPS Pharm Sci Tech, 2011; 12:1407-1419.
10. Haznedar S., Dortunc B., Preparation and evaluation of Eudragit microspheres containing acetazolamide, Int. J. Pharm.,2004; 269:131- 140.
11. Higuchi T., Mechanism of sustained action medication: theoretical analysis of rate of release of solid drugs dispersed in solid matrices, J. Pharm. Sci., 1963; 52:1145-1149.
12. Korsmeyer RW, Gurny R, Doelker E, Buri P, Peppas NA. Mechanisms of solute release from porous hydrophilic polymers Int. J. Pharm., 1983; 15:25-35.
13. Peppas N. Analysis of Fickian and non-Fickian drug release from polymers Pharm. Acta Helv., 1985; 60:110-111.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).