PROTECTIVE EFFECT OF ETHANOLIC EXTRACT FROM THE ROOT OF ARGYREIA SPECIOSA AGAINST GLOBAL CEREBRAL ISCHEMIC REPERFUSION INJURY IN RATS
Cerebral stroke is the principal reason of death without effective treatment in the world and recognized as the common cause of disability. Argyreia speciosa (Linn.f.) (Convolvulaceae, Synonyms: Argyreia nervosa) is widely distributed plant species in India. It is commonly known as Elephant creeper and Vryddhadaru. A. speciosa is a very valuable plant in the Ayurvedic system. In 'Rasayan' drug it has been used for the treatment of various neurological diseases. Its root taste is bitter and having the multiple uses like as a brain tonic, nootropic, anti-anxiety and anticonvulsant activity. The current study, plan to investigate the neuroprotective effect of ethanolic extract of A. speciosa root (ASEE) in a validate rat model of stroke known as global cerebral ischemic reperfusion injury (GCIRI). We divided 36 male Wistar rats to six experimental groups (n= 6). The group-I considered as sham control (no GCIRI), Group-II saline treated GCIRI, Group-III, IV, and V received ASEE (100, 200 and 400 mg/kg, p.o.) for 7 days prior to the induction of GCIRI while Group-VI termed as standard and it received quercetin (20 mg/kg, i.p.) 30 min prior induction of GCIRI. GCIRI produced the significant neurological deficit, sensorimotor dysfunction, decrease neurobehavioral parameters, increased cerebral infarction area and brain edema as compared with sham control rats. Seven days of pretreatment with ASEE markedly attenuates all the changes caused by GCIRI to the normal level. Our results proved that ASEE possess the protective effect on GCIRI induced stroke and aforementioned neuroprotection may be due to its antioxidant and anti-inflammatory property.
Keywords: Brain stroke, BCCAO, Antioxidants, Neuroprotection, Argyreia speciosa
2. Pandian JD, Sudhan P, Stroke epidemiology and stroke care services in India, Journal of Stroke, 2013; 15(3):128-134.
3. Byles JE, Francis JL, Chojenta CL, Hubbard IJ, Long-term survival of older Australian women with a history of stroke, Journal of Stroke and Cerebrovascular Diseases, 2015; 24:53-60.
4. Christophe BR, Mehta SH, Garton AL, Sisti J, Connolly ES, Current and future perspectives on the treatment of cerebral ischemia, Expert opinion on pharmacotherapy, 2017; 18:573-580.
5. Sallustio F, Motta C, Pizzuto S, Diomedi M, Giordano A, D'Agostino VC, Sama D, Mangiafico S, Saia V, CT angiography-based collateral flow and time to reperfusion are strong predictors of outcome in endovascular treatment of patients with stroke, Journal of neurointerventional surgery, 2017; 9:940-943.
6. Gong G, Xiang L, Yuan LB, Hu L, Wu W, Cai L, Yin L, Dong HL, Protective effect of glycyrrhizin, a direct hmgb1 inhibitor, on focal cerebral ischemia/reperfusion-induced inflammation, oxidative stress, and apoptosis in rats, PLoS One, 2014; 9(3):89450.
7. Manzanero S, Santro T, Arumugam TV, Neuronal oxidative stress in acute ischemic stroke: sources and contribution to cell injury, Neurochemistry International, 2013; 62:712-718.
8. Coyle JT, Puttfarcken P, Oxidative stress, glutamate, and neurodegenerative disorders, Science, 1993; 262(5134):689-695.
9. Mijajlovic MD, Shulga O, Bloch S, Covickovic-Sternic N, Aleksic V, Bornstein NM, Clinical consequences of aspirin and clopidogrel resistance: an overview, Acta neurologica Scandinavica, 2013; 128:213-219.
10. Zuo FT, Liu H, Wu HJ, Su N, Liu JQ, Dong AQ, The effectiveness and safety of dual antiplatelet therapy in ischemic cerebrovascular disease with intracranial and extracranial arteriostenosis in Chinese patients: A randomized and controlled trail, Medicine, 2017; 96:e5497.
11. Aiyer KN, Kolammal M, Pharamacognosy of ayurvedic drugs (kerala), Journal of pharmacy and pharmacology, 1964; 16(12):836.
12. Nadkarni AK. Indian Materia Medica. Bombay: Popular parkashan private Ltd; 1997. P. 137.
13. Girach RD, Aminuddin, Ahmad M, Medical ethnobotany of sundargarh, Orissa, India, Pharmaceutical Biology, 1998; 36:20-24.
14. Husain OPA, Viramani SP, Popli LN, Misra MM, Gupta GN, Srivastava Z, Singh AK. Dictionary of Indian medicinal plants. Lucknow: Central Institute of Medicinal and Aromatic Plants. 1992. P. 45-46.
15. Joseph A, Mathew S, Skaria BP, Sheeja EC, Medicinal uses and biological activities of Argyreia speciosa sweet (Hawaiian baby woodrose) - an overview, Indian Journal of Natural Products and Resources, 2011; 2:286-291.
16. Paulke A, Kremer C, Wunder C, Achenbach J, Djahanschiri B, Argyreia nervosa (Burm. f.): receptor proﬁling of lysergic acid amide and other potential psychedelic LSD-like compounds by computational and binding assay approaches, Journal of Ethnopharmacology, 2013; 148:492-497.
17. Singhal AK, Gupta H, Bhati VS, Wound healing activity of Argyreia nervosa leaves extract, International Journal of Applied and Basic Medical Research, 2011; 1(1):36-39.
18. Mishra SH, Chaturvedi SC, Antibacterial and antifungal activity of the oil and un-saponifiable matter of Argyreia speciosa sweet, Indian Drugs and Pharmaceuticals, 1978; 13:29-31.
19. Suvarna CM, Rao YN, Rao MP, Beeravali SR, Ravindranaik R, Hepatoprotective and anxiolytic activity of methanolic extract of Argyreia nervosa, International Journal of Universal Pharmacy and Bio Sciences, 2013; 2:56-67.
20. George M, Joseph L, Gupta H, Priya G, Anti-inflammatory and analgesic activity of Argyreia nervosa leaves extract, World Journal of Pharmaceutical Research, 2016; 5:2119-2127.
21. Lalan BK, Hiray RS, Ghongane BB, Evaluation of analgesic and anti-inflammatory activity of extract of holoptelea integrifolia and Argyreia speciosa in animal models, Journal of Clinical and Diagnostic Research, 2015; 9:01-04.
22. Vivek P, Jayakumari D, Jayasree P, Hypoglycaemic Effect of vriddhadaru Argyreia nervosa (Burm. f.) Boj. in alloxan induced diabetic rabbits, International Journal of Advanced Ayurveda, Yoga, Unani, Siddha and Homeopathy, 2016; 5:322-329.
23. Sareedenchai V, Wiwat C, Wongsinkongman P, Soonthornchareonnon N, In vitro testing of anti-HIV and antioxidative activities of Argyreia nervosa (Burm.f) Bojor leaves, Pharmaceutical Sciences Asia, 2014; 41:47-53.
24. Thakur J, Sharma S, Mukhija M, Kalia AN, Flavonoid fraction of Argyreia nervosa leaves with antiulcer potential in different experimental rat models, International Journal of Pharmaceutical Research and Bio-Science, 2013; 2:557-574.
25. Vyawahare NS, Bodhankar SL, Anticonvulsant activity of Argyreia speciosa in mice, Indian Journal of Pharmaceutical Sciences, 2009; 71:131-134.
26. Galani VJ, Patel BG, Central nervous system activity of Argyreia speciosa roots in mice, Research Journal of Pharmacy and Technology, 2009; 2:331-334.
27. Vyawahare NS, Pujari R, Kagathara V, Gangurde P, Bodhankar S, Hadambar A, Central nervous system activity of Argyreia speciosa, Journal of Pharmacy and Research, 2009; 8:152-158.
28. Harborne JB. Phytochemical methods. London: Chapman and Hall; 1987. P. 286.
29. Slinkard K, Singleton VL, Total phenol analyses: Automation and comparison with manual methods, American Journal of Enology and Viticulture, 1977; 28:49-55.
30. Chang CC, Yang MH, Wen HM, Chern JC, Estimation of total flavonoids content in propolis by two complementary colorimetric methods, Journal of Food and Drug Analysis, 2002; 10:178-182.
31. Farbiszewski R, Bielawski K, Bielawska A, Sobaniec W, Spermine protects in-vivo the antioxidant enzymes in transiently hypoperfused rat brain, Acta Neurobiologiae Experimentalis Journal, 1995; 55:253-258.
32. Longa EZ, Weinstein PR, Carlson S, Cummins R, Reversible middle cerebral artery occlusion without craniectomy in rats, Stroke, 1989; 20:84-91.
33. Hunter AJ, Hatcher J, Virley D, Nelson P, Irving E, Handubgham SJ, Parsons AA, Functional assessments in mice and rats after focal stroke, Neuropharmacology, 2000; 39:806-816.
34. Dunham NW, Miya TS, A note on a simple apparatus for detecting neurological deficit in rats and mice, Journal of Pharmaceutical Sciences, 1957; 46:208-210.
35. Lister J, Ethologically based animal models of anxiety disorders, Pharmacology and Therapeutics, 1990; 46:321-340.
36. Lister RG, The use of a plus-maze to measure anxiety in the mouse, Psychopharmacology, 1987; 92:180-185.
37. Bederson JB, Pitts LH, Germano SM, Nishimura MC, Davis RL, Bartkowski HM, Evaluation of 2,3,5-triphenyltetrazolium chloride as a stain for detection and quantification of experimental cerebral infarction in rats, Stroke, 1986; 17:1304-1308.
38. Hatashita S, Hoff JT, Salamat SM, Ischemic brain edema and the osmotic gradient between blood and brain, Journal of Cerebral Blood Flow & Metabolism, 1988; 8:552-559.
39. Hossmann KA, The two pathophysiologies of focal brain ischemia: implications for translational stroke research, Journal of cerebral blood flow and metabolism, 2012; 32:1310-1316.
40. Gelderblom M, Leypoldt F, Steinbach K, Behrens D, Choe CU, Siler DA, Arumugam TV, Orthey E, Gerloff C, Tolosa E, Magnus T, Temporal and spatial dynamics of cerebral immune cell accumulation in stroke, Stroke, 1999; 40:1849-1857.
41. Du Y, Zhang X, Ji H, Liu H, Li S, Li L, Probucol and atorvastatin in combination protect rat brains in MCAO model: upregulating Peroxiredoxin2, Foxo3a and Nrf2 expression. Neuroscience letters, 2012; 509:110-115.
42. Madamanchi NR, Vendrov A, Runge MS, Oxidative stress and vascular disease, Arteriosclerosis, Thrombosis and Vascular Biology, 2005; 25:29-38.
43. Drobyshevsky A, Luo K, Derrick M, Yu L, Du H, Prasad PV, Vasquez-Vivar J, Batinic-Haberle I, Tan S, Motor deficits are triggered by reperfusion-reoxygenation injury as diagnosed by MRI and by a mechanism involving oxidants, The Journal of neuroscience, 2012; 32:5500-5509.
44. Wicha P, Tocharus J, Janyou A, Jittiwat J, Changtam C, Suksamrarn A, Hexahydrocurcumin protects against cerebral ischemia/reperfusion injury, attenuates inflammation, and improves antioxidant defenses in a rat stroke model, PLoS ONE, 2017; 12(12):e0189211.
45. Talluri MR, Killari KN, Viswanadh NVS, Manepalli M, Konduri P, Bandaru KK, Protective effect of Canna indica on cerebral ischemia-reperfusion injury in rats, Agriculture and Natural Resources, 2017; 51(6):470-477.
46. Qiuyun Tu, Ranran Wang, Binrong Ding, Wei Zhong, Hui Cao, Protective and antioxidant effect of Danshen polysaccharides on cerebral ischemia/reperfusion injury in rats. International Journal of Biological Macromolecules, 2013; 60:268-271.
47. Hirvonen T, Virtamo J, Korhonen P, Albanes DP, Intake of flavonoids, carotenoids, vitamins C and E, and risk of stroke in male smokers, Stroke, 2000; 31:2301-2306.
48. Afshari L, Amani R, Soltani F, Haghighizadeh MH, Afsharmanesh MR, The relation between serum Vitamin D levels and body antioxidant status in ischemic stroke patients: A case-control study, Advanced Biomedical Research, 2015; 16(4):213.
49. Leinonen JS, Ahonen JP, Lonnrot K, Jehkonen M, Dastidar P, Molnar G, Low plasma antioxidant activity is associated with high lesion volume and neurological impairment in stroke, Stroke, 2000; 31:33-39.
50. Kozue K, Shinichi N, Sachiko J, Sachiyo S, Etsuko M, Yan X, Edaravone, a free radical scavenger, mitigates both gray and white matter damages after global cerebral ischemia in rats, Brain Research, 2009; 1279:139-146.
51. Shah ZA, Gilani RA, Sharma P, Vohora SB, Cerebroprotective effect of Korean ginseng tea against global and focal models of ischemia in rats, Journal of Ethnopharmacology, 2005; 101(1-3):299-307.
52. Liang G, Shi B, Luo W, Yang J, The protective effect of caffeic acid on global cerebral ischemia-reperfusion injury in rats, Behavioral and Brain Functions, 2015; 11:1-10.
53. Surapaneni S, Prakash T, Ansari A, Goli D, Neuroprotective effect of Clerodendron glandulosum against acute transient ischemia reperfusion cerebral injury in rats, Journal of Neurology and Neurorehabilitation Research, 2017; 2:14-20.
54. Collins RC, Dobkin BH, Choi DW, Selective vulnerability of the brain: New insights into the pathophysiology of stroke, Annals of Internal Medicine, 1989; 110:992-1000.
55. Ghosh A, Sarkar S, Mandal AK, Das N, Neuroprotective role of nano-encapsulated quercetin in combating ischemia-reperfusion induced neuronal damage in young and aged rats, PLoS ONE, 2013; 8(4):e57735.
56. Lei X, Chao H, Zhang Z, Lv J, Li S, Wei H, Xue R, Li F, Li Z, Neuroprotective effects of quercetin in a mouse model of brain ischemic/reperfusion injury via anti-apoptotic mechanisms based on the Akt pathway, Molecular Medicine Report, 2015; 12(3):3688-3696.
57. Dai Y, Zhang H, Zhang J, Yan M, Isoquercetin attenuates oxidative stress and neuronal apoptosis after ischemia/reperfusion injury via Nrf2-mediated inhibition of the NOX4/ROS/NF-κB pathway, Chemico-Biological Interactions, 2018; 25(284):32-40.
58. Liu J, Chen Q, Jian Z, Xiong X, Shao L, Jin T, Zhu X, Wang L, Daphnetin protects against cerebral ischemia/reperfusion injury in mice via inhibition of TLR4/NF-
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