EVALUATION OF LEARNING AND MEMORY ENHANCING ACTIVITY OF COCCINIA GRANDIS FRUITS IN RATS
Objectives: To assess the learning and memory enhancing activity of the Coccinia grandis fruits in rats using Elevated plus maze (EPM), Hebb-William maze (HWM) and Morris water maze (MWM ) and to evaluate brain Acetylcholine esterase activity, lipid peroxidation, Superoxide dismutase, Catalase, Glutathione.
Materials and methods: Wistar rats (100-150 gm) of either sex, were divided into 5 groups (n=6). Group-I (control) animals received vehicle, Group-II animals received diazepam(1 mg/kg i.p), Groups III ,IV and V animals received Coccinia grandis- 500 mg/kg p.o, 1000 mg/kg p.o and Piracetam (400 mg/kg i.p) respectively for 27 days, followed by diazepam (1 mg/kg i.p) single dose on 19th and 27th day. Assessment of transfer latency (TL), time taken to reach reward chamber (TRC) and swim latency (SL) was done on 16th, 17th, 18th, 19th and 27th day using EPM, HWM and MWM respectively. Rats were sacrificed on 28th day, brain acetylcholine esterase activity, lipid peroxidation, superoxide dismutase, glutathione and catalase levels were estimated. The data was analyzed by one way ANOVA followed by Dunnett’s test. P ≤ 0.05 was considered significant.
Result: Coccinia grandis decreased TL, TRC and SL in comparison to diazepam treated rats, decreased acetylcholine esterase activity and lipid peroxidation, and increased superoxide dismutase, glutathione and catalase in brain.
Conclusion: The Coccinia grandis enhanced learning and memory activity. This nootropic effect can be attributed to their antioxidant and neuroprotective property.
Keywords: Memory, EPM, HWM, MWM, Piracetam, Diazepam.
2. Srithi S. Memory enhancing medicinal herbs. J Pharm Sci Res. 2014; 6(10): 331-33.
3. Joshi P. Review on natural memory enhancers (nootropics). Uni J Eng Adv Sci. 2013; 1(1):8-18.
4. Pekamwar S, Kalyankar T, Kokate SS. Pharmacol activities of Coccinia grandis. J Appl Pharm Sci. 2013; 3(5):114-19.
5. Amir H, Uddin N, Abu S, Razaul H. Phytochemical and pharmacological screening of Coccinia grandis Linn. J Sci Innov Res. 2014; 3(1):65-71.
6. Petricia RI, Jeevan MBD, Venkat RN, Rizwan AS. Nootropic activity of fruit extracts of Litchi chinensis sonn (sapindaceae). Int J Pharm Tech. 2012; 4(3):4795-804.
7. Ashwini M, Nisha L, Shivaji B, Vedamurthy AB, Sam B. Invitro antioxidant and anti-inflammatory activity of Coccinia grandis. Int J Pharm Sci. 2012; 4(3):239-42.
8. Vijay CJ, Babasaheb SS. Memory enhancing activity of Taverniera cuneifolia (Roth) Arn: A substitute for commercial Liquorice. Int J Pharma Bio Sci. 2013; 4(2):277-85.
9. Meera S. Mamatha K. Assessement of learning and memory enhancing activity of Phoenix dactylifera (date) in rats. World J Pharma Res. 2014; 3(10):1215-25.
10. Talpate KA, Bhosale UA, Zambare MR, Somani RS. Neuroprotective and nootropic activity of Clitorea ternatea Linn. (Fabaceae) leaves on diabetes induced cognitive decline in experimental animals. J Pharm Bioallied Sci. 2014; 6(1):48-55.
11. Chakravarthi KK, Ramakrishna A. Beneficial effect of aqueous root extract of Glycyrrhiza glabra on learning and memory using different behavioural modes: experimental study. J Nat Sci Bio Med. 2013; 4(2):420-25.
12. Shivakumar L, Shivaraj GT, Rao VN, Shalam, Richa V. Evaluation of memory enhancing activity of SR-105 in experimental animals. Int J Res Ayur Pharm. 2011; 2(3):973-77.
13. Shibnath K, Nookala VNM, Chandra NS. Screening of novel polyphytoformulations, natural remedies for learning and memory enhancing properties in rat. Int J Nutr Pharmacol Neuro Dis. 2015; 5(1):13-19.
14. Nilofar SN, Somnath NM, Rahul SA, Chandrakant SM. Memory enhancing activity of Rose Alba in mice. Int J Green Pharma. 2009; 239-42.
15. Deshpande SV, Patil MJ, Parmar KK, Daswadkar SC, Khodade RB. A study of antioxidant activity of fruit extracts of Coccinia grandis L Voigt. Int J Drug Res Tech. 2011; 1(1):69-72.
16. Gupta R, Lalit KG, Pramod KM, Swapan KB. Effect of resveratrol on scopolamine-induced cognitive impairement in mice. Pharmacol Reports. 2012; 64:438-44.
17. Venkat NR, Basavaraj P, Nimhal Sk, Shantakumar SM, Satyanarayana S. Nootropic activity of tuber extract of Pueraria tuberose (Roxb). Ind J Expt Bio. 2008; 46:591-98.
18. Himanshu BS, Pravat KM, Subrat KB, Amrita B, Rakesh S. A new weapon for memory power: Elephantopus scaber (Linn.). Int J Nutri Pharmacol Neuro Dis. 2014; 4(1):64-68.
19. Rajiv G, Hemant KS. Nootropic potential of Alternanthera sessilis and Clerodendrum infortunatum leaves on mice. Asian Pacific J Tropic Dis. 2013; 4(4):1591-97.
20. Chandan R. A nootropic effect of Moringa oleifera on Ach and ChAT activity in colchicine induced experimental rat model of alzheimer’s disease: Possible involvement of antioxidants. Al Ameen J Med Sci. 2014; 7(2):125-33.
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