The POTENTIAL OF CAESALPINIA CRISTA LEAVES IN THE TREATMENT OF ULCERATIVE COLITIS IN LABORATORY ANIMALS
The aim of present investigation was to study the Ulcerative colitis effect of extracts of Caesalpinia crista in acetic acid induced experimental colitis in Sprague Dawley rats. Sprague Dawley rats were divided into nine groups (n=6). The rats were received 7 days pretreatment with chloroform, ethyl acetate, ethanolic extracts of C. crista 200 mg/kg and 400 mg/kg. Ulcerative colitis was induced by intrarectal administration of 1ml of 4% acetic acid solution on 8th day. Prednisolone (2 mg/kg) was used as standard drug administered orally for 3 days. After 48 hrs of colitis induction animals were sacrificed by cervical dislocation to remove colon and distal 5 cm of the colon was dissected. Macroscopical study, Ulcer index of the colon, colonic myeloperoxidase (MPO) and malondialdehyde (MDA) level in colon tissue and blood were studied. Intrarectal instillation of acetic acid caused enhanced ulcer index, myeloperoxidase and malondialdehyde. Ethanol extract of C.crista showed significant effect in lowering ulcer index as well as neutrophil infiltration at a dose of 400 mg/kg in acetic acid induced colitis. The present investigation demonstrates that the ethanol extract of C.crista is of potent therapeutic value in the amelioration of experimental colitis in rat by inhibiting the inflammatory mediator.
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