Evaluation of in-vitro antioxidant potential and in-vivo hepatoprotective activity of root extract of Quercus oblongata D. DON

  • Anita Singh Department of Pharmaceutical sciences Bhimtal campus Kumaun University. Uttarakhand. India
  • Manoj Bisht Department of Pharmaceutical sciences Bhimtal campus Kumaun University. Uttarakhand. India

Abstract

Objective: The main potential target is attempt to investigated evaluation of in-vitro antioxidant potential and in-vivo hepatoprotective activity of root extract of Quercus oblongata D. DON belonging to family fagaceae. Material & Methods: The root of plant was extracted by different solvents like n-hexane (NHEQO), Chloroform (CEQO), Ethyl acetate (EAQO) Hydroethanolic (HEEQO) and Ethanol (EEQO). The antioxidant activity (AA) was determined by the possible four complementary test assay methods namely total phenolic content, total flavonoids content, Inhibition of  2,2 diphenyl -1 picrylhydrazyl (DPPH) radicals and ABTS (2-2’- azinobis) radical scavenging activity or quenching activity, in the hepatoprotective experimental  animal albino wistar rats (120-180gm) were divided into 6 group, each group content 5, Group I: Received distilled water (5ml/kg. p.o) once daily, and served as normal control. Group II: Received paracetamol suspension (640 mg/kg suspended in 1% methyl cellulose; orally as toxin control. Group III: Received standard drug Silymarin (25 mg/kg. p.o.) + paracetamol suspension (640 mg/kg suspended in 1% methyl cellulose; orally once daily Group IV, V, VI administered HEECB at different doses300, 400, 500 mg/kg orally + paracetamol suspension (640 mg/kg suspended in 1% methyl cellulose; for 21 days. And collect blood from experimental animals by retrorbital puncture for estimation of biochemical parameters and other parameter also evaluate like physical histological changes in livers of rats. Results: Experimental finding reveal that Paracetamol produce significant change in physical (increase liver weight) biochemical (increase alkaline phosphate, serum glutamic oxalacetic transaminase, serum glutamic pyuruvic transaminase, total protein, total bilirubin, direct bilirubin and decrease the level of total protein and albumin) histological (damage to hepatocyte) and in liver parameters. Pretreatment with extract significantly minimization of physical, biochemical, histological and functional change induced by Paracetamol in liver. Conclusion: Experimental data and analysis of different parameter declare that hydroethanolic extract of Quercus oblongata could be a useful hepatoprotective agents and antioxidant potential.


Keywords: Clematis buchananiana, paracetamol, hepatoprotective, alkaline phosphate, serum glutamic oxalacetic transaminase, serum glutamic pyuruvic transaminase.

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Author Biographies

Anita Singh, Department of Pharmaceutical sciences Bhimtal campus Kumaun University. Uttarakhand. India

Department of Pharmaceutical sciences Bhimtal campus Kumaun University. Uttarakhand. India

Manoj Bisht, Department of Pharmaceutical sciences Bhimtal campus Kumaun University. Uttarakhand. India

Department of Pharmaceutical sciences Bhimtal campus Kumaun University. Uttarakhand. India

References

1. Akhtar N., Rashid A., Murad W., Bergmeier E. Diversity and use of ethno-medicinal plants in the region of Swat, North Pakistan. Journal of ethnobiology and ethnomedicine, 2013; 9(1):25.
2. Ahmed M.J., Murtaza G.A study of medicinal plants used as ethnoveterinary: Harnessing potential phytotherapy in Bheri, District Muzaffarabad (Pakistan). Journal of ethnopharmacology, 2015; 159:209-214.
3. Bhandari B.S., Riaz,T., Panwar R., Gusain P. Indigenous traditional knowledge Of health care system Adopted By The Bhotiya tribe And other inhabitants In A Part Of Garhwal Himalaya. World J. Pharm. Pharm. Sci, 2016; 5(12):802-817.
4. Shah A., Bharati K.A., Ahmad J., Sharma M.P. New ethnomedicinal claims from Gujjar and Bakerwals tribes of Rajouri and Poonch districts of Jammu and Kashmir, India. Journal of ethnopharmacology, 2015; 166:119-128.
5. Kanturski M., Zia A., Rafi M.A. The Lachnus of Pakistan with description of a new species (Hemiptera: Aphididae: Lachninae). Journal of Asia-Pacific Entomology, 2017; 20(4):1219-1227.
6. Osuagwu G.G.E., Eme C.F. The phytochemical composition and antimicrobial activity of Dialium guineense, Vitex doniana and Dennettia tripetala leaves. Asian Journal of Natural and Applied Sciences, 2013; 2(3):69-81.
7. Kumar A., Chahal K.K., Kataria D. A review on phytochemistry and pharmacological activities of Cyperus scariosus. Journal of Pharmacognosy and Phytochemistry, 2017; 6(1):510-517.
8. Srivastava V., Negi A.S., Kumar J.K., Gupta M.M., Khanuja, S. P. Plant-based anticancer molecules: a chemical and biological profile of some important leads. 2005.
9. Sharma N., Ashok P.K., Negi A., Lakshmayya B. A Review on Ethnobotany, Phytochemical and Pharmacological Dynamics of Prangos pabularia Lindl. Journal of Natural Remedies, 13(2), 68-75.Bioorganic & medicinal chemistry, 2013; 13(21):5892-5908.
10. Negi A., Dobhal K., Ghildiyal P. Antioxidant Potential and Effect of Extraction Solvent on Total Phenol Content, Flavonoids Content and Tannin Content of Ficus palmata Forssk. Int. J. Pharm. Sci. Rev. Res., 2018; 49(2):19-24
11. Pradhan S. C., Girish C. Hepatoprotective herbal drug, silymarin from experimental pharmacology to clinical medicine. Indian Journal of Medical Research, 2006; 124(5):491-504.
12. Akhilesh, Neeraj K., Bisht M. Antiepileptic activity of hydroethanolic extract of Thalictrum foliolosum on maximal electroshock (MES) and pentylenetetrazole (PTZ) induced seizure in rats. International journal of pharmacology & toxicology, 2017; 71-6.
13. Akhilesh, Neeraj K., Bisht M, In Vitro antioxidant acivity of Whole Plant Extracts of Thalictrum foliolosum Dc (Pilijari). Am.J.Pharmatech Res, 2016; 6:202-210.
14. Joshi S, Sati SC. Screening of antibacterial potentiality of Thalictrum foliolosum leaves extracts. Novus Natural Science Research 2014; 2, 3:7.
15. Mitchell M. F., Cantor S. B., Ramanujam N., Tortolero-Luna G., & Richards-Kortum R. Fluorescence spectroscopy for diagnosis of squamous intraepithelial lesions of the cervix. Obstetrics & Gynecology, 1999; 93(3):462-470.
16. Eidi A., Eidi M., Al-Ebrahim M., Rohani AH., Mortazavi P. Protective effects of sodium molybdate on carbon tetra chloride induced hepatotoxicity in rats. J Trace Elements Med Biol, 2011; 25:67-71.
17. Yeh YH., Hsieh Yl., Lee YT., Hsieh CH. Protective effects of cholestin against carbon tetrachloride- induced hepatotoxicity in rats. e-SPE, 2012; 48:551-558.
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Singh A, Bisht M. Evaluation of in-vitro antioxidant potential and in-vivo hepatoprotective activity of root extract of Quercus oblongata D. DON. JDDT [Internet]. 15Oct.2018 [cited 29Mar.2024];8(5-s):152-61. Available from: https://jddtonline.info/index.php/jddt/article/view/1925
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