Pesticidal residue analysis and phytochemical screening in leaves and roots of Barleria prionitis Linn.
In the present study emphasize on phytochemical screening in leaves and roots of Barleria prionitis Linn. The extract of plant materials total ash content, extractive value, water soluble ash, and loss on drying values were identified. Medicinal plant materials are liable to contain pesticide residues, which accumulate from agriculture practices, such as spraying, treatment of soils during cultivation and administration of fumigants during storage. It is therefore recommended that every sample of medicinal plants used for such type of studies should be analyzed for the pesticidal residues. Therefore in the present study parts of the herb used for their antidiabetic potential were analyzed for organochlorine pesticides, which are persistent and remain in the food chain for longer periods. The samples of leaf and root of Barleria prionitis were ground coarsely and then preceded for the extraction procedure. The sample of extracts were analysed for residues of organochlorine pesticides by Gas Chromatography (GC). Analysis were carried out on a Schimadzu Model 2010 Gas Chromatograph (GC) equipped with 63 NI electron capture detector (ECD) and a capillary column HP ultra 2. The instrument was supported by Lab Solution software. The pesticide residues detected in them in the GC were within the limits. For phytochemical screening, the ethanolic and hydroalcoholic extracts obtained were prepared and subjected to various qualitative tests in order to reveal the presence or absence of common phytopharmaceuticals by using standard tests.
Keywords: Phytochemical screening, Pesticidal residues, Barleria prionitis Gas Chromatography, Ash value, extractive value.
2. Chauhan B.L., Mitra S.K., Mohan A.R., Gopumadhavan, S., and Anturilikar, S.D. Development of High Performance Thin Layer Chromatography Fingerprint Technique and Bioassay to establish the shelf life of a Proprietary Herbal Formulation (PHF), an Ayurvedic Formulation Containing Multiple Ingredients of Herbal Origin. Indian Drugs. 1994; 31(7):333.
3. Chevreuil M.E. Note sur le sucre de diabete. Ann Chin (Paris). 1815; 95:319.
4. Chinoy N.J. et al (1993): Essential techniques in reproductive physiology and endocrinology, Ananda Publisher, Ahmedabad. (Course material provided for refresher course).
5. Chopra, R.N., Nayar, S.L., Chopra, I.C. (1999) : Glossary of Indian Medicinal Plants. CSIR : 33.
6. Cignarella A., Nastasi M., Cavalli E. and Puglisi L., Novel lipid-lowering properties of Vaccinium myrtillus L. leaves, a traditional antidiabetic treatment, in several models of rat dyslipidaemia. a comparison with ciprofibrate. Thromb Res. 1996; 84:311-322.
7. Cottenie,A.,Dhaese, A.,Camerlink, R., Plants quality response to uptake of polluting elements.Qualitas plantarum.26:293-319
8. Damtoft, S. et al (1982): Structural Renzion of Barlerin and acetyl barlerin. Tetrahedron. Lett. 1976; 23(40):4155-4156.
9. Datta P.C., Biswas C. Pharmacognostic study of the leaf and bark of Barleria Prionitis Linn. Quart. J. Crude Drug Res. 1968; (8):1161.
10. Davidson M. B., Diabetes Mellitus: Diagnosis and treatment, 3rd ed.New York NY: Churchill Livingstone. 1991
11. Davis, J., (1993): New chemical entities disappoint in 1992. Scrip Review 1992. PJB Publications, Rechmond, 20-21.
12. Dawsen A., ark V., Rapid stick method for determining blood glucose.Brit. Med. Jour. 1965; 1:243
13. Day, C., Cartwright, T., Provost, J., and Baiey, C.J. Hypoglycemic effect of Momordica charantia extracts. Planta Med. 1990; 56:426-429.
14. De Meyer J., Action de la secretion interne du pancreas sur differents organs et en particular sur la secretion Renale. Arch Fisiol 1909; 7:96-99.
15. De Pasquale R., Ragusa S., Lauk L., Barbera R., Galati E. M. “Effect of Cadmium of germination, growth and active principle contents of Matricaria revcutita. L.”, Pharm. Res. Comm. 1988; 20,Suppl.V:151-154.
16. De Pasquale R., Ragusa S., lauk L., Barbera R., Galati E. M. “ Effect of Cadmium of germination, growth and active principle contents of Datura metel L.”, Toxicological and Environmental Chemistry 1988; 23:121-127.
17. De Pasquale R., Ragusa S., Lauk L., Barbera R., Galati E. M., (): “Effect of Cadmium on germination, growth and active principle contents of Achillea millefolium L.” Pharms Res. comm. 1988; 20, Supple V:145-149.
18. De Smet PAGM Health risks of herbal remedies, Drug safety, 1995; 13:81-93.
19. Deb S. and Mandal, S.K. Review of the analysis of medicinal plant by TLC. Modern Approaches. Indian Drugs. 1999; 36(11):687.
20. DeFronzo R. A., Pathogenesis of type-2 diabetes.In:Am Diab Assn: Annual Rev Diab, 1998; 1-93.
21. Dhandapani S., Subramanian V.R., Rajagopal S., Namasivayam N. Hypolipidemic effect of Cuminum cyminum L. on alloxous induced diabetic rats. Pharmacol Res. 2002; 46(3):251-5.
22. Dhar M.L., Dhar M.M., Dhawan B.N., Mehrotra b.N., and Ray. C. Screening of Indian Plants for biological activity, Part-1. Indian Jour. of Exp. Bio. Vol. 1968; 6:232.
23. Dobson, M.: Experiments and observations on the urine in diabetes. In: Medical observations and inquiries by a society of physicians in London, Bd. 1776; 5(S):298-316.
24. Dutta, A.C., Botany (1999): Oxford University Press, Calcutta, 6th Edition, 8th Impression: 535, 581.
25. Egede, L.E., Ye, X. Zheng, D and Silverstein, M.D The prevalence and pattern of complementary and alternative medicine use in individuals with diabetes. Diabetes Care. 2002; 25:324-329.
26. Eisenberg D.M., Kessler R.C., Foster C., Norlock F.E., Calkins, D.R. and Deibanco, .L. Unconventional medicine in the United States. Prevalence, costs, and patterns of use. N.Engl. J. Med. 1993; 328:246-252.
27. El-Missiry M.A. and El-Gindy A.M.(2000): Amelioration of induced diabetes mellitus and oxidative stress inrats by oil of Eruca sativa seeds.Ann. Nutr Metab.44:97-100.
28. Flannigan B. Hui S. C, The occurrencxe of aflatoxin-producing strains of Aspergillus flavus in the mould floras of ground spices. Journal of Applied Bacteriology 1976; 41:411.
29. Fossati, P. and Lererzo, P. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin. Chem. 1982; 28:2077-2080.
30. Frazier W. C., Westhoff D. C., Food Microbiology. Mac Grave Hills B. C. 1976;194.
31. Galati E. M., Lauk L., Ragusa S., Barbera R., De Pasquale R, “ Effects of Copper on the growth and active principle content of Datura metel L.”, Int. Crude Drug Res. 1989; 27(2):113-117.
32. Garg M.C., Bansal, D.D., Protective antioxidant effect of vitamin C and E in streptozotocin induced diabetic rats. Indian J. Exp. Biol. 2000; 38:101-104.
33. Gawas, S. and Grampurohit, N.D. HPTLC Analysis of some Ayurvedic formulation containing vasaka and pepper. Indian Drugs. 1999; 36(3):175.
34. Geeta M. and Wahi, A.K. Identity of root of Barleria prionitis Linn. Ind. Jour. of Nat. Produ. 1997; 13(2):14-16.
35. Harborne J B. “Phytochemical Methods” Chapman and Hall Ltd, London, 1973; 49-188.
36. Trease G E, Evans W C. Pharmacognosy 11th edition, Baillere Tindall, London, 1989; 45-50.
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