STUDY ON ANTICANCER EFFECT OF SYNTHETIC BIOGENIC SOURCE OF GERMACRENE A
Objective: Based on obtaining germacrene A, which is the analogue of synthetic β-elemene, by means of synthetic biology，this experiment aims to explore the anti-tumor effect of germacrene A.
Results: After administration, the volume of T24 cells was significantly reduced. The shape of the T24 cells gradually changed from wedge-shaped and polygonal to round, and the T24 cells soon fell off, atrophied and disintegrated. After Tet blue staining, a large number of T24 cells after administration were observed to be dead; It was found by means of MTT assay that T24 cells were inhibited to varying degrees by the effects of different concentrations of germacrene A.
Conclusion: The above experimental results show that the synthetic biological source of germacrene A has obvious inhibitory effect on bladder cancer T24 cells, and this effect has obvious time and concentration dependence.
Keywords：Germacrene A; Synthetic drugs; Antitumor; β-elemene
 Tan W, Lu J, Huang M, et al. Anti-cancer natural products isola-ted from chinese medicinal herbs [J]. Chinese Medicine, 2011; 6(1):27.
 Paddon C J, Westfall P J, Pitera D J, Benjamin K, Fisher K, Mcphee D et al. High-level semi-synthetic production of the potent antimalarial artemisinin [J]. Nature, 2013, 496(7446):528-532.
 Zhang R, Tian A, Shi X, et al. Downregulation of IL-17 and IFN-γ in the optic nerve by beta-elemene in experimental autoimmune encephalomyelitis [J]. Int Immunopharmacol, 2010; 10 (7):738-743.
 Zhang X, Li Y, Zhang Y, et al. Beta-elemene blocks epithelial mesenchymal transition in human breast cancer cell linee MCF-7 through Smad3-Mediated down-regulation of nuclear transcription factors [J]. PLo S One, 2013; 8(3):e58719.
 Shi H, Liu L, Chen L, et al. Inhibition of tumor growth by β-elemene through dow nregulation of the expression of u PAR, MMP-2, and MMP-9 in a murine intraocular melanoma modet [J]. Melanoma Res, 2015; 25(1):15-21.
 Mao Y, Zhang J, Hou L, et al. The effect of beta-elemene on alphatubulin polymerization in human hepatoma Hep G2 cells [J]. Chin J Cancer Res, 2013; 25(6):770-776.
 Li CL, Chang L, Guo L, et al. β-elemene Induces Caspase-dependent Apoptosis in Human Glioma Cell in vitro through the Up-regulation of Bax and Fas / Fas L and Downregulation of Bcl-2[J]. Asian Pac Asian Pac J Cancer Prev, 2014; 15(23):10407-10412.
 Li Q, Wang G, Huang F, et al. Antineoplastic effect of beta-elemene on prostate cancer cells and other types of solid tumour cells [J]. J Pharm Pharmacol, 2010; 62(8):1018-1027.
 Chen W, Lu Y, Wu J, et al. Beta-elemene inhibits melanoma growth and metastasis via suppressing vascular endothelial growth factor-mediated angiogenesis [J]. Cancer Chemother Pharmacol, 2011; 67(4):799-808.
 Andrianantoandro E, Basu S, Karig DK, et al. Synthetic biology: new engineering rules for an emerging discipline. Mol Syst Biol, 2006; 2(1):2006.0028.
 Zhou T, Huang LQ, Lü DM. Modes and types of protecting natural resources of Chinese medicine [J]. China J Chin Mater Med, 2008; 33:1353−1356.
 Xu H, Li L, Fu J, et al. Reversion of Multidrug Resistance in a Chemoresistant Human Breast Cancer Cell Line by β-Elemene[J]. Pharmacology, 2012; 89(5/6):303-312.
 Yao C, Tu Y, Jiang J, et al. β-elemene reverses the drug resistance of lung cancer A549/DDP cells via the mitochondrial apoptosis pathway [J]. Oncol Rep, 2014; 31(5):2131-2138.
 Zhao HM, Medema MH. Standardization for natural product synthetic biology. Nat Prod Rep, 2016; 33(8):920-924.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeÂ The Effect of Open Access).