FORMULATION AND EVALUATION OF DOXORUBICIN CONTAINING NANOGELS FOR DELIVERY TO CANCER CELLS

  • Manish Kumar Research Scholar, College of Pharmacy, Sri Satya Sai University of Technology and Medical Sciences, Village-Pachama, District- Sehore, Madhya Pradesh-466001, India.
  • Hemant K. Sharma Dean & Professor, College of Pharmacy, Sri Satya Sai University of Technology and Medical Sciences, Village-Pachama, District- Sehore, Madhya Pradesh-466001, India.

Abstract

The objective of this study is to prepare nanogels were prepared via charged gellan gum. It was prepared by in situ cross linking reaction between two oppositely charged materials by green method without use of chemical cross linking agents. The prepared nanogels were characterized by Dynamic light scattering, scanning electron microscopy, differential scanning calorimetry and X- Ray diffractometry. The prepared formulation had average particle size of 226 nm with polydispersity index of 0.3. The doxorubicin loaded nanogel demonstrated sustained release for 20 h. The prepared nanogels were hemocompatible and cyctocompatible as revealed by hemocompatibility and MTT assay respectively. All results confirmed that these nanogels can be used for cancer treatment.


Keywords: Nanogel, Chitosan, Gellan gum, Doxorubicin, Cancer.

Keywords: Nanogel, Chitosan, Gellan gum, Doxorubicin

Downloads

Download data is not yet available.

Author Biographies

Manish Kumar, Research Scholar, College of Pharmacy, Sri Satya Sai University of Technology and Medical Sciences, Village-Pachama, District- Sehore, Madhya Pradesh-466001, India.

Research Scholar, College of Pharmacy, Sri Satya Sai University of Technology and Medical Sciences, Village-Pachama, District- Sehore, Madhya Pradesh-466001, India.

Hemant K. Sharma, Dean & Professor, College of Pharmacy, Sri Satya Sai University of Technology and Medical Sciences, Village-Pachama, District- Sehore, Madhya Pradesh-466001, India.

Dean & Professor, College of Pharmacy, Sri Satya Sai University of Technology and Medical Sciences, Village-Pachama, District- Sehore, Madhya Pradesh-466001, India.

References

1. Anitha A, Divya RVV, Krishna R, Sreeja V, Selvamurugan N, Nair SV. Synthesis, characterization, cytotoxicity and antibacterial studies of chitosan,O-carboxymethyl and N, O-carboxymethyl chitosan nanoparticles. Carbohyd Polym. 2009; 78(4):672–7.
2. Jayakumar R, Chennazhi KP, Muzzarelli RAA, Tamura H, NairSV, Selvamurugan N. Chitosan conjugated DNA nanoparticles in gene therapy. Carbohyd Polym. 2010; 79(1):1–8.
3. Janssen PE, Lindeberg B, Sandford PA. Structural studies of gellan gum, an extra cellular polysaccharide elaborated by Pseudomonas eloda. Carbohyd Res. 1983; 124(1):135-9.
4. Malcolm A, O’Neill R, Selvendran R, Morris VJ. Structure of the acidic extracellular gelling polysaccharide produced by Pseudomonas elodea. Carbohyd Res. 1983;124(1):123–33.
5. Sasaki Y, Akiyoshi K. Nanogel engineering for new nanobiomaterials: from chaperoning engineering to biomedical applications. Chemical record (New York, N.Y.) 2010; 10(6):366-76.
6. Rhee Y, Mansour HM. Nanopharmaceuticals I: nanocarrier systems in drug delivery. Int J of Nanotech. 2011; 8(1-2):84-114.
7. Wu X, Mansour HM. Nanopharmaceuticals II: application of nanoparticles and nanocarrier systems in pharmaceutics and nanomedicine. Int J of Nanotech. 2011; 8(1-2):115-45.
8. Gupta P, Vermani K, Garg S. Hydrogels: from controlled release to pH-responsive drug delivery. Drug discovery today. 2002; 7(10):569-79.
9. Matsumura Y, Maeda HA. New concept for macromolecular therapeutics in cancer chemotherapy: mechanism of tumoritropic accumulation of proteins and the antitumor agent smancs. Cancer Res. 1986; 46(12):6387-92.
10. Anitha A, Deepagan VG, Divya R VV, Deepthy M, Nair SV, Jayakumar R. Preparation, characterization, in vitro drug release and biological studies of curcumin loaded dextran sulphate–chitosan nanoparticles. Carbohyd Polym. 2011; 84(3):1158-64.
11. O’Leary RR, Watkins WD, Guess WL. Comparative chemical and toxicological evaluation of residual ethylene oxide in sterilized plastics. J Pharm Sci. 1969; 58(8):1007-10.
12. Madane RG, Mahajan HS. Curcumin-loaded nanostructured lipid carriers (NLCs) for nasal administration: design, characterization, and in vivo study. Drug Del. 2014; 23(4):1-9.
13. Zahr AS, Davis CA, Pishko MV. Macrophage uptake of core shell nanoparticles surface modified with poly (ethylene glycol). Langmuir. 2006; 22(19):8178-85.
14. Peppas NA. Analysis of fickian and non fickian drug release from polymers Pharm Acta Helv. 1985; 60:110–11.
15. Sarika PR, Nirmala RJ. Preparation and characterisation of gelatin–gum arabic aldehyde nanogels via inverse miniemulsion technique. Int Journal of Biol Mac. 2015; 76:181-7.
16. Zhao L, Feng S. Enhanced oral bioavailability of paclitaxel formulated in vitamin E-TPGS emulsified nanoparticles of biodegradable polymers: in vitro and in vivo studies. J of Pharm Sci. 2010; 99(8):3552–60.
Statistics
48 Views | 91 Downloads
How to Cite
Kumar, M., & Sharma, H. K. (2018). FORMULATION AND EVALUATION OF DOXORUBICIN CONTAINING NANOGELS FOR DELIVERY TO CANCER CELLS. Journal of Drug Delivery and Therapeutics, 8(5), 178-183. https://doi.org/10.22270/jddt.v8i5.1890