Effect of Eclipta alba and Ocimum sanctum on haloperidol induced parkinsonism
The aim of the study is protective effect of compound Eclipta alba and Ocimum sanctum on Parkinsonism induced mice by haloperidol injection. Parkinsonism is neurodegenerative disease due to the deficiency of dopamine in brain. The pathological hallmark of Parkinson’s disease in the cell loss within substantia nigra pars compacta (SNpc) region and the disease is charactrised by bradykinesia, rigidity, postural instability, orofacial dyskinesia, muscular stiffness and tremor1. Mice were injected 1mg/kg haloperidol and then treated with test and standard substance for 15 days. The impairment in catatonia in mice were tested using catatonic activity. Biochemical analysis of brain homogenate was performed so ass to assess brain Thiobarbituric acid reactive substance (TBARS) level and reduced glutathione (GSH) and TNF-α level were measured to assess total oxidative stress. EA 300mg/kg and OS 400mg/kg show slightly change in catatonic activity in mice while EA 600mg/kg and 800mg/kg significantly change in catatonic activity. Furthermore, Eclipta alba and Ocimum sanctum prevent the haloperidol induced changes in the level of brain TBARS, GSH and TNF-α. From the results we conclude that Eclipta alba and Ocimum sanctum has protective action against impairment in catatonic activity and pathological damage due to oxidative stress induced by intraperitoneally injection of haloperidol in mice.
Keywords: Eclipta alba, Ocimum Sanctum, Parkinsonism, Anti-oxidant.
2. Manoharan S, Guillemin GJ, Abiramasundari RS, Essa MM, Akbar M, Akbar MD, The role of reactive oxygen species in the pathogenesis of Alzheimer’s disease, Parkinson’s disease and Huntington’s disease: A mini review, Oxidative medicine and cellular Longevity, 2016; 15:8590578.
3. Raudenska M, Gumulee J, Babula P, Stracina T, Sztalmachova M, Polanska H, Adam V, Kizek R, Novakova M, Masarik M, Haloperidol Cytotoxicity and Its Relation to Oxidative Stress, Mini-Reviews in Medicinal Chemistry, 2013; 13:14
4. Pimentel C, Nascimento LB, Pousada CR, Menezes RA, Oxidative Stress in Alzheimer’s and Parkinson’s Diseases: Insights from the Yeast Saccharomyces cerevisiae, Oxidative Medicine and Cellular Longevity, 2012; 9:32146
5. Houshmand G, Tarahomi S, Arzi A, Goudarzi M, Bahadoram M, Nooshabadi MR, Red Lentil Extract: Neuroprotective Effects on Perphenazine Induced Catatonia in Rats, Journal of Clinical and Diagnostic Research. 2016; 10(6):FF05-FF08.
6. Thakur VD, Mengi SA: Neuropharmacological profile of Eclipta alba (Linn.) Hassk. Journal of Ethnopharmacology 2005; 102:23–31
7. Khanna N, Bhatia J, Antinociceptive action of Ocimum sanctum (Tulsi) in mice: possible mechanisms involved, J Ethnopharmacol. 2003; 88(2-3):293-6.
8. Ahmed M, Ahamed R, Aladakatti RH, Ghodesawar MAG, Effect of benzene extract of Ocimum sanctum leaves on cauda epididymal spermatozoa of rats, Iranian Journal of Reproductive Medicine, 2011; 9(3):177-186.
9. Velmurugan, et al., Anti-parkinsonism activity of saraca asoca by haloperidol induced model, Journal of harmonized research in Pharmacy 2015; 4(4):281-295.
10. Sathish kumar M, Farzana SK, Nadendla Ramarao, Evaluation of anti-catatonic effect of leaf extracts of Tragia plukenetti R.Smith on Phenothiazine induced catatonia in rats, RJPBCS 2014; 5(1):832.
11. Polydoro M, Schroder N, Lima MNM, Caldana F, Laranja DC, Bromberg E, Roesler R, Quevedo J, Moreira JCF. Haloperidol- and clozapine-induced oxidative stress in the rat brain, Pharmacology Biochemistry and Behaviour, 2004; 78(4):751-756. https://doi.org/10.1016/j.pbb.2004.05.018
12. Hoffman DC, Donovan H. Catalepsy as a rodent model for detecting antipsychotic drugs with extrapyramidal side effect liability. Psychopharmacology 1995; 120:128-33.
13. Moratalla R, Xu M, Tonegawa S, Graybiel AM. Cellular responses to psychomotor stimulant and neuroleptic drugs are abnormal in mice lacking the D1 dopamine receptor. Proc Natl Acad Sci USA 1996; 93:14928-33.
14. Wadenberg ML, Soliman A, VanderSpek SC, Kapur S. Dopamine D2 receptor occupancy is a common mechanism underlying animal models of antipsychotics and their clinical effects. Neuropsychopharmacology 2001; 25:633-41.
15. Góngora-Alfaro JL, Moo-Puc RE, Villanueva-Toledo JR, Alvarez-Cervera FJ, Bata-García JL, Heredia-López FJ et al. Long-lasting resistance to haloperidol-induced catalepsy in male rats chronically treated with caffeine. Neurosci Lett 2009; 463:210-4.
16. Chitra V, Manasa K, Tamilanban T, Narayanan J. Effect of Canscora decussata extract against the neurochemical and behavioral changes induced by 1-methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine in mice. Indian Journal of Pharmaceutical Education and Research. 2018; 52(1). DOI: 10.5530/ijper.52.1.10
17. Pimentel C, Nascimento B L, Pousada CR, Menezes RA. Oxidative Stress in Alzheimer's and Parkinson's Diseases: Insights from the Yeast Saccharomyces cerevisiae. Oxidative Medicine and cellular Longevity. 2012; 9:132146.
18. Schulz JB, Lindenau J, Seyfried J, Dichgans J. Glutathione, oxidative stress and neurodegeneration. Eur. J. Biochem. 2000; 267:4904-4911.
19. Ahmad MP, Arshad H, Kalam NB, Anshu M, Hasin MA, Shadma W. Effect of the Aqueous Extract of Mentha Arvensis on Haloperidol Induced Catalepsy in Albino Mice. Journal od clinical and diagnostic research. 2012; 6(3):542-546.
20. Pahaye DB, Bum EN, Taiwe GS, Ngoupaye GT, Ngoupaye GT, Sidiki N et al., Neuroprotective and Antiamnesic Effects of Mitragyna inermis Willd (Rubiaceae) on Scopolamine-Induced Memory Impairment in Mice. Behavioural Neurology. 2017; 11:5952897.
21. Thakur KS, Parkash A, Bisht R, Bansal PK. Beneficial effect of candesartan and lisinopril against haloperidol-induced tardive dyskinesia in rat. Journal of the Renin-Angiotensin Aldosterone system. 2014; 16(4):917-929.
22. Lewitus GM, Pribiag, Duseja R, Hilaire MS, Stellwagen D. An Adaptive Role of TNF in the Regulation of Striatal Synapses. The Journal of Neuroscience. 2014; 34(18):6146-6155.
23. Bishnoi M, Chopra K, Kulkarni SK. Differential striatal levels of TNF-α, NFκB p65 subunit and dopamine with chronic typical and atypical neuroleptic treatment: Role in orofacial dyskinesia. Neuro-Psychopharmacology & Biological Psychiatry 2008; 32:1473-1478.
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