CHLOROFORM FRACTION OF PARKIA JAVANICA BARK POSSESSES ANTIBACTERIAL ACTIVITY AGAINST MULTIDRUG RESISTANT GRAM NEGATIVE BACTERIA PREDOMINANTLY FOUND IN SKIN WOUND
Abstract
Aim: To evaluate the antibacterial activity of Parkia javanica against gram negative MDR bacterial strains which are predominantly found in skin wound. Methods: The 5 different solvent fractions of Parkia javanica were screened for antibacterial activity against gram negative multi drug resistant bacterial strains namely Enterobacter aerugenes, Pseudomonas aeruginosa and Klebsiella pneumonia by serial dilution technique. Growth kinetics study was performed and percentage of ROS production was measured by NBT reduction assay. Results: The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were obtained with a range of IC100 0.08-0.31 mg/ml in case of MDR bacterial strains. The lag phase of all extract treated bacteria is extended compared to untreated cells. The normalized % of ROS is increased in presence of Parkia javanica extract. Conclusions: This study suggests that, chloroform fraction of Parkia javanica possesses promising antimicrobial substances which are having activity against MDR bacterial strains and ROS induced bacterial cell damage could be the possible mediator of its antimicrobial activity.
Keywords: Parkia javanica, antibacterial activity, MDR bacterial strains, growth curve, ROS.
Keywords:
Parkia javanica, antibacterial activit, MDR bacterial strains, growth curve, ROS
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References
REFERENCES
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17. Nikolic M., Vasic S., Durđevic J., Stefanovic O., Comic L., Antibacterial and anti-biofilm activity of ginger (Zingiber officinale (roscoe)) ethanolic extract. Kragujevac J. Sci 2014; 36(2014):129-136.
18. Bhattacharya D., Samanta S., Mukherjee A., Santra C. R., Ghosh A. N., Niyogi S. K., Karmakar P., Antibacterial Activities of Polyethylene Glycol, Tween 80 and Sodium Dodecyl Sulphate Coated Silver Nanoparticles in Normal and Multi-Drug Resistant Bacteria. J Nanosci and Nanotech 2012; 12:1–9.
19. Pramanik A., Laha D., Bhattacharya D., Pramanik P., Karmakar P., A novel study of antibacterial activity of copper iodide nanoparticle mediated by DNA and membrane damage. Colloids and Surfaces B: Biointerfaces 2012; 96:50-55.
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26. James A., Imlay J.A., Cellular defences against superoxide and hydrogen peroxide. Annu Rev Biochem 2008; 77:755-776.
1. Cosgrove SE, Carmeli Y. The impact of antimicrobial resistance on health and economic outcomes. Clin infect Dis 2003; 36(11):1433-7.
2. Hancock E.W., Mechanisms of action of newer antibiotics for gram positive pathogens. Lancet Infect Dis 2005; 5:209-218.
3. Lomovskaya O., Bostian K. A., Practical applications and feasibility of efflux pump inhibitors in the clinic-a vision for applied use. Biochem pharmacol 2006; 71:910-918.
4. Coates A., Hu Y., Bax R., Page C. The future challenges facing the development of new antimicrobial drugs. Nat. Rev. Drug Discov 2010; 1: 895-910.
5. Bader M.S, Diabetic foot infection. Am Fam Physician 2008; 78(1):71-79.
6. Demetrio L.V.J., Jeannie I.A., Juliana J.M.P., Esperanza C. C., Antibacterial activities of ethanol extracts of Philippine medicinal plants against multidrug-resistant bacteria. Asian Pac J Trop Biomed 2015; 5(7):532-540.
7. Trentin DS, Giordani RB, Zimmer KR, da Silva AG, da Silva MV, Correia MTS, Baumvol IJR, Macedo AJ. Potential of medicinal plants from the Brazilian semi-arid region (Caatinga) against Staphylococcus epidermidis planktonic and biofilm lifestyles. Journal ethnopharmacology 2012; 137:327-335.
8. Gunasekar M, Geemon K, Mariwala SJ. Health benefits of bioactive molecules from spices and aromatic plants. Journal of Spices and Aromatic Crops 2012; 21:87-101.
9. Kavitha D, Niranjali S. Inhibition of Enteropathogenic Escherichia coli Adhesion on host epithelial cells by Holarrhena antidysenteriea (L.) WALL. Phototherapy Research 2009; 23:1229-1236.
10. Birdi T, Daswani P, Brijesh S, Tetali P, Natu A, Anita N. Newer insights into the mechanism of action of Psidium guajava L. Leaves in infectious diarrhoea. BMC complementary and alternative medicine 2010; 10-33.
11. Sinha J., Medicinal plants of Manipur, Mass and Sinha Manipur Cultural Integration Conference, Imphal, 1996.
12. Majumder K., Dutta B.K., Roy D., Inventory and status of medicinal trees of Tripura, Indian Medicinal Plants. Editor P. C. Trivedi. Avishkar publishers, Distributors, Jaipur 2009; 93- 123.
13. Bhardwaj S., Gakhar SK, Ethnomedicinal plants used by the tribals of Mizoram to cure cuts & wounds. Indian J. Traditional knowledge 2005; 4(1):75- 80.
14. Saha S, Karmakar P and Sil SK. Antibacterial activities of Parkia javanica extract against multidrug resistant gram negative bacteria predominantly found in skin wound. Int J Pharm Bio Sci 2018; 8(1):96-102.
15. Sil SK, Saha S and Karmakar P. Reactive Oxygen Species as possible mediator of antimicrobial activity of Parkia javanica, against bacterial species predominantly found in chronic wound. J. Drug Deliv. Ther. 2018; 8(1):43-47. https://doi.org/10.22270/jddt.v8i1.1657
16. Saha S, Basu Mullick J, Ray Choudhury P, Saha P, Chakraborty D and Sil SK. Anti-Vibrio Activity of Parkia javanica: Studies on MIC, MBC, Growth Curve Analysis and ROS Generation on Four Vibrio cholarae Strains. Int. J. Curr. Microbiol. App. Sci. 2016; 5(8):538-544.
17. Nikolic M., Vasic S., Durđevic J., Stefanovic O., Comic L., Antibacterial and anti-biofilm activity of ginger (Zingiber officinale (roscoe)) ethanolic extract. Kragujevac J. Sci 2014; 36(2014):129-136.
18. Bhattacharya D., Samanta S., Mukherjee A., Santra C. R., Ghosh A. N., Niyogi S. K., Karmakar P., Antibacterial Activities of Polyethylene Glycol, Tween 80 and Sodium Dodecyl Sulphate Coated Silver Nanoparticles in Normal and Multi-Drug Resistant Bacteria. J Nanosci and Nanotech 2012; 12:1–9.
19. Pramanik A., Laha D., Bhattacharya D., Pramanik P., Karmakar P., A novel study of antibacterial activity of copper iodide nanoparticle mediated by DNA and membrane damage. Colloids and Surfaces B: Biointerfaces 2012; 96:50-55.
20. Anbukkarasi M., Dhamotharan R., Janarthanam B., Studies on phytochemical screening, tannins content and antibacterial activity from leaf and callus extracts of Memecylon umbellatum. Asian J Pharm Clin Res 2017; 10(5):265-269.
21. Lai C C., Lee K., Xiao Y.H., Ahmad N., Veeraraghavan B., Thamlikitkul V., High burden of antimicrobial drug resistance. Asia J Glob Antimicrob Resist 2014; 2(3):141-147.
22. Quisumbing E., Medicinal plants of Philippines. Quezon: Katha Publishing Co., Inc (1978).
23. Cabiscol E., Tamarit J., Ros J., Oxidative stress in bacteria and protein damage by reactive oxygen species. Int. Microbiol 2000; 3:3-8.
24. De Orue Lucana D.O., Wedderhoff I., Groves M. R., ROS mediated signalling in bacteria: zinc-containing cys-x-x-cys redox centres and iron based oxidative stress. J Sig Trans 2012; doi: 10.1155/2012/605905.
25. Storz G., imlay J.A., Oxidative stress. Curr opin Microbial 1999; 2:188-194.
26. James A., Imlay J.A., Cellular defences against superoxide and hydrogen peroxide. Annu Rev Biochem 2008; 77:755-776.
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Saha S, Karmakar P, Sil S. CHLOROFORM FRACTION OF PARKIA JAVANICA BARK POSSESSES ANTIBACTERIAL ACTIVITY AGAINST MULTIDRUG RESISTANT GRAM NEGATIVE BACTERIA PREDOMINANTLY FOUND IN SKIN WOUND. JDDT [Internet]. 15Sep.2018 [cited 8Dec.2022];8(5):184-9. Available from: http://jddtonline.info/index.php/jddt/article/view/1847
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