RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ASPIRIN AND OMEPRAZOLE IN BULK AND DOSAGE FORM
RP-HPLC method was developed for the determination of Omeprazole (OME) & Aspirin (ASP) in bulk and dosage form. Mobile phase use for the separation of OME & ASP is methanol and 0.05%OPA in water (pH= 3.5) with ratio of 60:40. The Colum used as C18 (Cosmosil) 4.6×150mm and flow rate 0.7mL/min. UV detector is used and the detection wavelength is 231nm. Retention time of OME and ASP are 4.61 & 8.03 min, respectively. This method was validated as per ICH guidelines. Linearity was observed at 10-50µg/mL of OME and 20-100µg/mL of ASP. The % RSD is found to be less than 2%.The resolution between OME and ASP is 11.55 and the tailing factors of both are less than 2.0.Therotical plates for OME and ASP are 5060, and 9367, respectively. Total run time is 15min. The developed RP-HPLC method was accurate, precise, selective and rapid for simultaneous estimation of Omeprazole and Aspirin in the pharmaceutical dosage form.”
Keyword: Omeprazole, Aspirin, RP-HPLC validation.
4. Indian Pharmacopoeia, Government of India, Ministry of Health and Family Welfare, Published by the controller of publication, Delhi 1, 1996, 469.
5. Kranthi KK, Supriya D, Divya D, Rani D, Neelima Munni G, International journal of pharmaceutical Investigations and Research Analytical method development and validation for the estimation of aspirin and omeprazole using RP-HPLC method, ICJPIR, 2017; 4(1).
6. Saravanan V., Revathi R., Meera N. Method development and validation for the simultaneous estimation of lycopene and ubidecarenone by RP-HPLC in combined pharmaceutical dosage form. Journal of Drug Delivery and Therapeutics, 2016; 6(5):46-51. https://doi.org/10.22270/jddt.v6i5.1295
7. Practical HPLC methods developments ACS short course attendees’ onl. Available from:www.bioanalytical.com/NWL/pdfs/acs.pd f. Accessed on 2006 Jan.
8. Notes for guidance on guidance on validation of analytical procedures: Methodology (CPMP/ICH/281/95).
9. Ravisankar P, Rajyalakshmi G, Devadasu C, Devala Rao G, Instant tips for right and effective approach to solve HPLC trouble shooting, Journal of chemical and pharmaceutical sciences. 2014; 7(3):259-274.
10. Javali B, Sravanthi B, Rao BM, Gopi K, Vamsi K, Rao AK, Prasanthi T, Global journal of Pharmacy & pharmaceutical Science, Development and Validation of UV Spectrophotom- etric Method for Simultaneous Estimation of Omeprazole and Domperidone in Capsule Dosage forms. 2017; 1(2):1-4
11. Gousuddin M, Sengupta P, Tripathi V, Das A, Malaysian Journal of Analytical Sciences, Malaysian Journal of Analytical Sciences, 2016; 20(2):247-257.
12. Singh D, Yadav H, Hinge M, Patel A, J Pharm Sci Bioscientific Res. Development and Validation of Analytical Methods for Simultaneous Estimation of Rosuvastatin, Clopidogrel and Aspirin in Pharmaceutical Dosage Form. 2016; 20(2):247-257.
13. Foram J. Chodvadiya, Thula K, Maheshwari D, International Journal of Recent Scientific Research, Simultaneous Estimation of Aspirin and Lansoprazol by RP–HPLC method, 2016; 6(4):3385-3390.
14. Kayesh P, ARahman, Sultan M, et al. Development and Validation of a RP-HPLC Method for the Quantification of Omeprazole in Pharmaceutical Dosage Form. J. Sci. Res. 2013; 5(2):335-3425.
15. Bhowmick M., Bhowmick P., Sengodan T., Thangavel S. Development and validation of bioanalytical RP HPLC method for the estimation of metoprolol tartrate in rabbit plasma after transdermal and oral administration: application in pharmacokinetic studies. Journal of Drug Delivery and Therapeutics, 2015; 5(4):43-53. https://doi.org/10.22270/jddt.v5i4.1118
16. Kumar S, Jamadar L, Bhat K, Musmade P. A Ssantharaju, Udupa N, Analytical method development and validation for Aspirin, J. International Journal of ChemTech Research, 2010; 2(1):389-399.
17. Khristi A, Mardia R, Suhagia B, UV Spectrophotometric method development and validation of first derivative method for simultaneous estimation of sildenafil citrate and aspirin in bulk and tablet dosage form, J. Indo American Journal of Pharmaceutical Research, 2015; 2837-2843.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeÂ The Effect of Open Access).