PHYTOCHEMICAL STANDARDIZATION AND ANTIOXIDANT POTENTIAL OF AYURVEDA FORMULATION DARVYADI RASKRIYA

  • Biresh Kumar Sarkar Central Ayurveda Research Institute for Respiratory Disorder, (CCRAS, Ministry of AYUSH, Govt. of India) Motibag Road, Patiala, Punjab, India, 147001.
  • Ravi Kumar Central Ayurveda Research Institute for Respiratory Disorder, (CCRAS, Ministry of AYUSH, Govt. of India) Motibag Road, Patiala, Punjab, India, 147001.
  • Vikas Kumar Central Ayurveda Research Institute for Respiratory Disorder, (CCRAS, Ministry of AYUSH, Govt. of India) Motibag Road, Patiala, Punjab, India, 147001.
  • Reeta Reeta Central Ayurveda Research Institute for Respiratory Disorder, (CCRAS, Ministry of AYUSH, Govt. of India) Motibag Road, Patiala, Punjab, India, 147001.
  • Shashi Pal Manav Bharati University, Solan, HP, India.
  • C. Murali Krishna Regional Ayurveda Research Institute for Skin Disorders, (CCRAS, Ministry of AYUSH, Govt. of India), Vijayawada, India.
  • SC Verma Pharmacopoeial Laboratory for Indian Medicine (PLIM) Ghaziabad, UP, India
  • Ravindra Singh Central Council for Research in Ayurvedic Sciences (CCRAS Ministry of AYUSH, Govt. of India), New Delhi-110058 India
  • Ramaiah Maddi Dept. of Pharmacognosy, Hindu College of Pharmacy, Amaravathi Road, Guntur 522002, A.P., India.

Abstract

The study represents phytochemical standardization and antioxidant potential of ayurveda formulation Darvyadi Raskriya. The study involves development of high-performance thin-layer chromatographic (HPTLC) method for analysis of formulation. The study utilizes analysis of glycyrrhizin in formulation which is the phytoconstituent of Glycyrrhiza glabra one of the component of formulation. The sample in ethanol was applied on aluminium TLC plates using Linomat 5 spray (CAMAG). Linear ascending development was performed in twin trough glass chamber saturated with mobile phase. The mobile phase consisted of ethyl acetate-methanol-formic acid (10:5:1 v/v/v). The spectrodensitometric detection was performed at the wavelength of 254 nm. The regression analysis was found to be linear with r2=0.997 in the concentration range 5-25 ppm. The antioxidant activity of formulation was also found to be significant as compared to control.

Keywords: Standardization, Glycyrrhiza glabra, Glycyrrhizin, HPTLC, Antioxidant.

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Author Biographies

Biresh Kumar Sarkar, Central Ayurveda Research Institute for Respiratory Disorder, (CCRAS, Ministry of AYUSH, Govt. of India) Motibag Road, Patiala, Punjab, India, 147001.

Central Ayurveda Research Institute for Respiratory Disorder, (CCRAS, Ministry of AYUSH, Govt. of India) Motibag Road, Patiala, Punjab, India, 147001.

Ravi Kumar, Central Ayurveda Research Institute for Respiratory Disorder, (CCRAS, Ministry of AYUSH, Govt. of India) Motibag Road, Patiala, Punjab, India, 147001.

Central Ayurveda Research Institute for Respiratory Disorder, (CCRAS, Ministry of AYUSH, Govt. of India) Motibag Road, Patiala, Punjab, India, 147001.

Vikas Kumar, Central Ayurveda Research Institute for Respiratory Disorder, (CCRAS, Ministry of AYUSH, Govt. of India) Motibag Road, Patiala, Punjab, India, 147001.

Central Ayurveda Research Institute for Respiratory Disorder, (CCRAS, Ministry of AYUSH, Govt. of India) Motibag Road, Patiala, Punjab, India, 147001.

Reeta Reeta, Central Ayurveda Research Institute for Respiratory Disorder, (CCRAS, Ministry of AYUSH, Govt. of India) Motibag Road, Patiala, Punjab, India, 147001.

Central Ayurveda Research Institute for Respiratory Disorder, (CCRAS, Ministry of AYUSH, Govt. of India) Motibag Road, Patiala, Punjab, India, 147001.

Shashi Pal, Manav Bharati University, Solan, HP, India.

Manav Bharati University, Solan, HP, India.

C. Murali Krishna, Regional Ayurveda Research Institute for Skin Disorders, (CCRAS, Ministry of AYUSH, Govt. of India), Vijayawada, India.

Regional Ayurveda Research Institute for Skin Disorders, (CCRAS, Ministry of AYUSH, Govt. of India), Vijayawada, India.

SC Verma, Pharmacopoeial Laboratory for Indian Medicine (PLIM) Ghaziabad, UP, India
Pharmacopoeial Laboratory for Indian Medicine (PLIM) Ghaziabad, UP, India
Ravindra Singh, Central Council for Research in Ayurvedic Sciences (CCRAS Ministry of AYUSH, Govt. of India), New Delhi-110058 India
Central Council for Research in Ayurvedic Sciences (CCRAS Ministry of AYUSH, Govt. of India), New Delhi-110058 India
Ramaiah Maddi, Dept. of Pharmacognosy, Hindu College of Pharmacy, Amaravathi Road, Guntur 522002, A.P., India.

Dept. of Pharmacognosy, Hindu College of Pharmacy, Amaravathi Road, Guntur 522002, A.P., India.

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How to Cite
Sarkar, B. K., Kumar, R., Kumar, V., Reeta, R., Pal, S., Krishna, C. M., Verma, S., Singh, R., & Maddi, R. (2018). PHYTOCHEMICAL STANDARDIZATION AND ANTIOXIDANT POTENTIAL OF AYURVEDA FORMULATION DARVYADI RASKRIYA. Journal of Drug Delivery and Therapeutics, 8(4), 331-334. https://doi.org/10.22270/jddt.v8i4.1806