STUDY ON BIOSYSTEMATIC AND BIOACTIVITY OF Nocardiopsis flavescencs RRMVCBNR OBTAINED FROM NICHE HABITATS OF VALPARAI HILL STATION

  • M Vineeth Assistant Professor, Department of Biotechnology, Rathinavel Subramaniam College of Arts and Science, Coimbatore -641402, Tamilnadu, India.
  • R Ragunathan Director, Centre for Bioscience and Nanoscience Research, Coimbatore -641021, Tamilnadu, India

Abstract

In this work, the soil samples were collected niche habitats of Valparai hill station to screen the diversity of actinomycetes. The actinobacterial were isolated by serial dilution and plating method on starch peptone agar media. In totally 8 different morphological were isolated on the basis of colony characteristics on starch peptone agar and dominative isolate were screened and plated on point inoculation. RRMVCBNR 1 isolate was biosystamatically characterized on the basis of microscopic, colony morphology, biochemical and phenotypic studies. Phenotypic studies indicated that strains belonged to genus of Nocardiopsis which was further confirmed by genotypic studies based on 16S rRNA gene sequences followed by phylogenetic tree construction. 16S rRNA gene sequences of strain used in this study exhibited sequence similarity in the range of 99-100% with those of selected isolate and it was identified as of Nocardiopsis flavescencs RRMVCBNR. The sequences of Nocardiopsis flavescencs 16S rRNA genes were deposited in genbank http://www.ncbi.nlm.nih.gov/genbank and received the accession number MG287120.The biological activity of Nocardiopsis flavescencs strain showed detectable antibacterial activity against Staphylococcus aureus and Klebsiella pneumonia has been studied.

Keywords: Nocardiopsis flavescencs, Starch peptone agar, Muller Hinton Agar, Mountain soil.SEM

Downloads

Download data is not yet available.

Author Biographies

M Vineeth, Assistant Professor, Department of Biotechnology, Rathinavel Subramaniam College of Arts and Science, Coimbatore -641402, Tamilnadu, India.

Assistant Professor, Department of Biotechnology, Rathinavel Subramaniam College of Arts and Science, Coimbatore -641402, Tamilnadu, India.

R Ragunathan, Director, Centre for Bioscience and Nanoscience Research, Coimbatore -641021, Tamilnadu, India
Director, Centre for Bioscience and Nanoscience Research, Coimbatore -641021, Tamilnadu, India

References

1. Meyer J, “Nocardiopsis, a new genus of the order Actinomycetales” Int. J. Syst Bacteriol, 1976; 26:487-493.
2. Srinivasan M C, Laxman RS, Deshpande M V, “Physiology and nutrition aspects of actinomycetes – an overview” World J. Microb. Biotechnol, 1991, 7:171-184.
3. Ravi Kumara, Smita Zinjardea, Vaishali Javdekarb, Tahsin Bennura, Ameeta, “Nocardiopsis species: Incidence, ecological roles and adaptations”Microbiological Research, 2015; 174:33-47
4. Anuprita A, Deshmukh, Narendra N, Vidhale, “Characterization of Novel Antibacterial Actinomycetes Strain N8 from Saline Soil of Vidarbha Region” Int. J. Life Sci. Biotech. Pharm. Res, 2015; 4(1):1-4
5. Chen YG, Cui XL, Kroppenstedt RM, Stackebrandt E, Wen ML, Xu LH, “Nocardiopsis quinghaiensis sp. nov isolated from saline soil in China” Int. J. Syst. Evol. Microbiol, 2008; 58:699-705
6. Bouras N, Meklat A, Zitouni A, Mathieu F, Schumann P, Sproer C, “Nocardiopsis algeriensis sp. nov an alkalitolerant actinomycete isolated from Saharan soil”.Antonie Van Leeuwenhoek, 2015; 107; 313-320.
7. Santhoshkumar S, Nagarajan N, AM fungal association in the Rhizosphere soil of some Pteridophytic plant species in Valparai Hills, Western Ghats of Tamilnadu, India, Int. J. of Life Sciences, 2014; 3:201-206.
8. Meena B, Rajan LA, Vinithkumar NV, Kirubagaran R, “Novel marine actinobacteria from emerald Andaman and Nicobar Islands: a prospective source for industrial and pharmaceutical byproducts” BMC Microbiol, 2013; 13:145.
9. Meklat A, Sabaou N, Zitouni A, Mathieu F, Lebrihi A, “Isolation, taxonomy, and antagonistic properties of halophilic actinomycetes in Saharan soils of Algeria” Appl.Environ. Microbiol, 2011; 77:6710-6714.
10. Hotam S Chaudhary, Jayprakash Yadav, Anju R Shrivastava, Smriti Singh, Anil K Singh, Natrajan Gopalan, “Antibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city of central India)”, 2013; 4(2):118-123
11. Tahsin Bennur, Ameeta Ravi Kumar, Smita Zinjarde, Vaishali Javdekar, “Nocardiopsis species: Incidence, ecological roles and adaptations” Microbiological Research, 2015; 174:33-47
12. Shade A, Hogan CS, Klimowicz AK, Linske M, McManus PS, Handelsman J, “Culturing captures members of the soil rare biosphere” Environmental Microbiology, 2012; 14:2247-2252.
13. Gayathri A, Madhanraj P, Panneerselvam A, “Diversity, Antibacterial Activity And Molecular Characterization of Actinomycetes Isolated From Salt Pan Region of Kodiakarai,Nagapattinam DT” Asian J. Pharm. Tech, 2011; 1(3):79-81
14. Jeffrey LSH, Sahilah AM, Son R. Tosiah S, “Isolation and screening of actinomycetes from Malaysian soil for their enzymatic and antimicrobial activities” J. Trop. Agric. and Fd. Sc, 2007; 35(1):159–164
15. Ali Mohammed Abdullah Bawazir1, Ahmed Al-Haddad, Abdullah AL-Mahdi, Manjula Shantaram, “Actinomycetes from Mountains of Hadhramout – Yemen” Int.J.Curr.Microbiol.App.Sci, 2017; 8:3521-3530
16. Mukesh Sharma, Pinki Dangi, Meenakshi Choudhary, “Actinomycetes: Source, Identification, and Their Applications,” Int.J.Curr.Microbiol.App.Sci, 2014; 3:801-832
17. Joseph Devadass B, Gabriel Paulraj M, Ignacimuthu S, Agastian Simiyon Theoder P , Al Dhabi NA, “Identification of Antimicrobial Compounds from Streptomyces sp. Isolated from Western Ghats Soil in Tamil Nadu,” EC Microbiology, 2017, 222-231.
18. Hozzein WN, Goodfellow M, “Nocardiopsis arabia sp. nov., a halotolerant actinomycete isolated from a sand-dune soil,” Int. J. Syst. Evol. Microbiol, 2008; 58:2520-2524.
19. Tian S, Yang Y, Liu K, Xiong Z, Xu L, Zhao L, “Antimicrobial metabolites from a novel halophilic actinomycete Nocardiopsis terrae YIM 90022,” Nat. Prod. Res, 2014; 28:344-346.
20. Jie Li, Jian Yang, Wen-Yong Zhu, Jie He, Xin-Peng Tian, Qiong Xie,Si Zhang , Wen-Jun Li, “Nocardiopsis coralliicola sp. nov., isolated from the gorgonian coral, Menella praelonga,” International Journal of Systematic and Evolutionary Microbiology, 2012; 62:1653-1658
21. Sunita C,Mesta Onkarappa R, “Characterization and Antimicrobial Potential of Marine Actinomycetes From Estuaries of Uttara Kannada District, Karnataka,” Journal of Drug Delivery & Therapeutics, 2017; 6:27-37
22. Virali MS, Vennila S, Vignesh S, Vikash K,Vijayakumar R, Panneerselvam A, “Antagonistic activity of actinomcyetes isolated from Marudhamalai Hills, Western Ghats,” J. Soil Biol. Ecol, 2007, 27; 1(2):19-25.
23. Arifuzzaman M, KhatunM R, Rahman H, “Isolation and screening of actinomycetes from Sundarbans soil for antibacterial activity,” Afr. J. Biotechnol, 2010; 9:4615-4619.
24. Basavaraj KN, Chandrashekhara S, Shamarez AM, Goudanavar PS, Manvi FV, “Isolation and morphological characterization of antibiotic producing actinomycetes,” Trop. J. Pharm. Res, 2010; 9:231-236.
25. Deepa S, Panneerselvam A, Dhanasekaran D, Thajuddin N, Vijayakumar R, “Diversity and antimicrobial potential of actinobacteria from saltpan environment,” Global Ad. Res. J. Microbiol, 2010; 1:140-148.
26. Kavitha A, Vijayalakshmi M, Sudhakar P, Narasimha G, “Screening of actinomycete strains for the production of antifungal metabolites,” Afr. J.Microbiol. Res, 2011; 4:27-32.
27. Monisha K, Renu S, “Selective isolation of rare actinomycetes producing novel antimicrobial compounds,” Int. J. Adv. Biotechnol. Res, 2011; 2:357-375.
28. Mythili B, Ayyappa Das MA, “Studies on antimicrobial activity of Streptomyces spp. isolates from tea plantation soil,” Res. J. Agri. Sci, 2011; 2:104-106.
29. Nithya B, Ponmurugan P, “Studies on actinomycetes diversity in Eastern Ghats (Yercaud Hills) of Southern India for secondary metabolite production,” Int. J. Agri. Res, 7:152-159.
30. Thakur D, Yadav A, Gogoi BK, Bora TC, “Isolation and screening of Streptomyces in soil of protected forest areas from the states of Assam and Tripura, India, for antimicrobial metabolites” J. Med. Mycol, 2007; 17:242-249.
Statistics
55 Views | 81 Downloads
How to Cite
Vineeth, M., & Ragunathan, R. (2018). STUDY ON BIOSYSTEMATIC AND BIOACTIVITY OF Nocardiopsis flavescencs RRMVCBNR OBTAINED FROM NICHE HABITATS OF VALPARAI HILL STATION. Journal of Drug Delivery and Therapeutics, 8(4), 282-290. https://doi.org/10.22270/jddt.v8i4.1792