STUDY ON BIOSYSTEMATIC AND BIOACTIVITY OF Nocardiopsis flavescencs RRMVCBNR OBTAINED FROM NICHE HABITATS OF VALPARAI HILL STATION
In this work, the soil samples were collected niche habitats of ValparaiÂ hill station to screen the diversity of actinomycetes. The actinobacterial were isolated by serial dilution and plating method on starch peptone agar media. In totally 8 different morphological were isolated on the basis of colony characteristics on starch peptone agar and dominative isolate were screened and plated on point inoculation. RRMVCBNR 1 isolate was biosystamatically characterized on the basis of microscopic, colony morphology, biochemical and phenotypic studies. Phenotypic studies indicated that strains belonged to genus of Nocardiopsis which was further confirmed by genotypic studies based on 16S rRNA gene sequences followed by phylogenetic tree construction. 16S rRNA gene sequences of strain used in this study exhibited sequence similarity in the range of 99-100% with those of selected isolate and it was identified as of Nocardiopsis flavescencs RRMVCBNR. The sequences of Nocardiopsis flavescencs 16S rRNA genes were deposited in genbank http://www.ncbi.nlm.nih.gov/genbank and received the accession number MG287120.The biological activity of Nocardiopsis flavescencs strain showed detectable antibacterial activity against Staphylococcus aureus and Klebsiella pneumonia has been studied.
Keywords: Nocardiopsis flavescencs, Starch peptone agar, Muller Hinton Agar, Mountain soil.SEM
2. Srinivasan M C, Laxman RS, Deshpande M V, â€œPhysiology and nutrition aspects of actinomycetes â€“ an overviewâ€ World J. Microb. Biotechnol, 1991, 7:171-184.
3. Ravi Kumara, Smita Zinjardea, Vaishali Javdekarb, Tahsin Bennura, Ameeta, â€œNocardiopsis species: Incidence, ecological roles and adaptationsâ€Microbiological Research, 2015; 174:33-47
4. Anuprita A, Deshmukh, Narendra N, Vidhale, â€œCharacterization of Novel Antibacterial Actinomycetes Strain N8 from Saline Soil of Vidarbha Regionâ€ Int. J. Life Sci. Biotech. Pharm. Res, 2015; 4(1):1-4
5. Chen YG, Cui XL, Kroppenstedt RM, Stackebrandt E, Wen ML, Xu LH, â€œNocardiopsis quinghaiensis sp. nov isolated from saline soil in Chinaâ€ Int. J. Syst. Evol. Microbiol, 2008; 58:699-705
6. Bouras N, Meklat A, Zitouni A, Mathieu F, Schumann P, Sproer C, â€œNocardiopsis algeriensis sp. nov an alkalitolerant actinomycete isolated from Saharan soilâ€.Antonie Van Leeuwenhoek, 2015; 107; 313-320.
7. Santhoshkumar S, Nagarajan N, AM fungal association in the Rhizosphere soil of some Pteridophytic plant species in Valparai Hills, Western Ghats of Tamilnadu, India, Int. J. of Life Sciences, 2014; 3:201-206.
8. Meena B, Rajan LA, Vinithkumar NV, Kirubagaran R, â€œNovel marine actinobacteria from emerald Andaman and Nicobar Islands: a prospective source for industrial and pharmaceutical byproductsâ€ BMC Microbiol, 2013; 13:145.
9. Meklat A, Sabaou N, Zitouni A, Mathieu F, Lebrihi A, â€œIsolation, taxonomy, and antagonistic properties of halophilic actinomycetes in Saharan soils of Algeriaâ€ Appl.Environ. Microbiol, 2011; 77:6710-6714.
10. Hotam S Chaudhary, Jayprakash Yadav, Anju R Shrivastava, Smriti Singh, Anil K Singh, Natrajan Gopalan, â€œAntibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city of central India)â€, 2013; 4(2):118-123
11. Tahsin Bennur, Ameeta Ravi Kumar, Smita Zinjarde, Vaishali Javdekar, â€œNocardiopsis species: Incidence, ecological roles and adaptationsâ€ Microbiological Research, 2015; 174:33-47
12. Shade A, Hogan CS, Klimowicz AK, Linske M, McManus PS, Handelsman J, â€œCulturing captures members of the soil rare biosphereâ€ Environmental Microbiology, 2012; 14:2247-2252.
13. Gayathri A, Madhanraj P, Panneerselvam A, â€œDiversity, Antibacterial Activity And Molecular Characterization of Actinomycetes Isolated From Salt Pan Region of Kodiakarai,Nagapattinam DTâ€ Asian J. Pharm. Tech, 2011; 1(3):79-81
14. Jeffrey LSH, Sahilah AM, Son R. Tosiah S, â€œIsolation and screening of actinomycetes from Malaysian soil for their enzymatic and antimicrobial activitiesâ€ J. Trop. Agric. and Fd. Sc, 2007; 35(1):159â€“164
15. Ali Mohammed Abdullah Bawazir1, Ahmed Al-Haddad, Abdullah AL-Mahdi, Manjula Shantaram, â€œActinomycetes from Mountains of Hadhramout â€“ Yemenâ€ Int.J.Curr.Microbiol.App.Sci, 2017; 8:3521-3530
16. Mukesh Sharma, Pinki Dangi, Meenakshi Choudhary, â€œActinomycetes: Source, Identification, and Their Applications,â€ Int.J.Curr.Microbiol.App.Sci, 2014; 3:801-832
17. Joseph Devadass B, Gabriel Paulraj M, Ignacimuthu S, Agastian Simiyon Theoder P , Al Dhabi NA, â€œIdentification of Antimicrobial Compounds from Streptomyces sp. Isolated from Western Ghats Soil in Tamil Nadu,â€ EC Microbiology, 2017, 222-231.
18. Hozzein WN, Goodfellow M, â€œNocardiopsis arabia sp. nov., a halotolerant actinomycete isolated from a sand-dune soil,â€ Int. J. Syst. Evol. Microbiol, 2008; 58:2520-2524.
19. Tian S, Yang Y, Liu K, Xiong Z, Xu L, Zhao L, â€œAntimicrobial metabolites from a novel halophilic actinomycete Nocardiopsis terrae YIM 90022,â€ Nat. Prod. Res, 2014; 28:344-346.
20. Jie Li, Jian Yang, Wen-Yong Zhu, Jie He, Xin-Peng Tian, Qiong Xie,Si Zhang , Wen-Jun Li, â€œNocardiopsis coralliicola sp. nov., isolated from the gorgonian coral, Menella praelonga,â€ International Journal of Systematic and Evolutionary Microbiology, 2012; 62:1653-1658
21. Sunita C,Mesta Onkarappa R, â€œCharacterization and Antimicrobial Potential of Marine Actinomycetes From Estuaries of Uttara Kannada District, Karnataka,â€ Journal of Drug Delivery & Therapeutics, 2017; 6:27-37
22. Virali MS, Vennila S, Vignesh S, Vikash K,Vijayakumar R, Panneerselvam A, â€œAntagonistic activity of actinomcyetes isolated from Marudhamalai Hills, Western Ghats,â€ J. Soil Biol. Ecol, 2007, 27; 1(2):19-25.
23. Arifuzzaman M, KhatunM R, Rahman H, â€œIsolation and screening of actinomycetes from Sundarbans soil for antibacterial activity,â€ Afr. J. Biotechnol, 2010; 9:4615-4619.
24. Basavaraj KN, Chandrashekhara S, Shamarez AM, Goudanavar PS, Manvi FV, â€œIsolation and morphological characterization of antibiotic producing actinomycetes,â€ Trop. J. Pharm. Res, 2010; 9:231-236.
25. Deepa S, Panneerselvam A, Dhanasekaran D, Thajuddin N, Vijayakumar R, â€œDiversity and antimicrobial potential of actinobacteria from saltpan environment,â€ Global Ad. Res. J. Microbiol, 2010; 1:140-148.
26. Kavitha A, Vijayalakshmi M, Sudhakar P, Narasimha G, â€œScreening of actinomycete strains for the production of antifungal metabolites,â€ Afr. J.Microbiol. Res, 2011; 4:27-32.
27. Monisha K, Renu S, â€œSelective isolation of rare actinomycetes producing novel antimicrobial compounds,â€ Int. J. Adv. Biotechnol. Res, 2011; 2:357-375.
28. Mythili B, Ayyappa Das MA, â€œStudies on antimicrobial activity of Streptomyces spp. isolates from tea plantation soil,â€ Res. J. Agri. Sci, 2011; 2:104-106.
29. Nithya B, Ponmurugan P, â€œStudies on actinomycetes diversity in Eastern Ghats (Yercaud Hills) of Southern India for secondary metabolite production,â€ Int. J. Agri. Res, 7:152-159.
30. Thakur D, Yadav A, Gogoi BK, Bora TC, â€œIsolation and screening of Streptomyces in soil of protected forest areas from the states of Assam and Tripura, India, for antimicrobial metabolitesâ€ J. Med. Mycol, 2007; 17:242-249.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeÂ The Effect of Open Access).