Application of ImageJ for processing Shilajit exposed PBMC images
Objective: In this study, application of ImageJ for processing Shilajit exposed PBMC images were studied.
Methods: In present study experiment was designed with human PBMC treated with Shilajit in high concentration (18mg/ml).Digital images were taken after one hour exposure with Shilajit and image processing steps were implemented.
Results: Acquired images from Shilajit exposed human PBMC had low contrast and substantial background noise as media was mixed with Shilajit. So images were processed by adjusting brightness and contrast, applying median filter, thresholding and watershed algorithm.
Conclusion: A high concentration of shilajit (18mg/ml) was detrimental to human PBMC. ImageJ can be efficiently used to process and extract information from low resolution images.
Keywords: ImageJ, Shilajit, PBMC
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