Application of ImageJ for processing Shilajit exposed PBMC images

  • Madhurima Ghosh Department of Biotechnology, PES Institute of Technology, Bangalore, India
  • Jhinuk Chatterjee Department of Biotechnology, PES University, Bangalore, India
  • V. Krishnamurthy Department of Biotechnology, PES University, Bangalore, India

Abstract

Objective: In this study, application of ImageJ for processing Shilajit exposed PBMC images were studied.

Methods: In present study experiment was designed with human PBMC treated with Shilajit in high concentration (18mg/ml).Digital images were taken after one hour exposure with Shilajit and image processing steps were implemented.

Results: Acquired images from Shilajit exposed human PBMC had low contrast and substantial background noise as media was mixed with Shilajit. So images were processed by adjusting brightness and contrast, applying median filter, thresholding and watershed algorithm.

Conclusion: A high concentration of shilajit (18mg/ml) was detrimental to human PBMC. ImageJ can be efficiently used to process and extract information from low resolution images.

Keywords: ImageJ, Shilajit, PBMC

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Author Biographies

Madhurima Ghosh, Department of Biotechnology, PES Institute of Technology, Bangalore, India

Department of Biotechnology, PES Institute of Technology, Bangalore, India

Jhinuk Chatterjee, Department of Biotechnology, PES University, Bangalore, India

Department of Biotechnology, PES University, Bangalore, India

V. Krishnamurthy, Department of Biotechnology, PES University, Bangalore, India

Department of Biotechnology, PES University, Bangalore, India

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How to Cite
Ghosh, M., Chatterjee, J., & Krishnamurthy, V. (2018). Application of ImageJ for processing Shilajit exposed PBMC images. Journal of Drug Delivery and Therapeutics, 8(4), 87-89. https://doi.org/10.22270/jddt.v8i4.1774