IMMUNOMODULATORY EFFECT OF PARKIA JAVANICA EXTRACT ON INTRACELLULAR EXPRESSION OF IL-6, IL-8, IL-12 AND TNF-α

  • S Saha Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.
  • M Dinda Department of Life Science and Biotechnology, Jadavpur University, 188, Raja S. C. Mallick Road, Kolkata, West Bengal-700032, India.
  • P Karmakar Department of Life Science and Biotechnology, Jadavpur University, 188, Raja S. C. Mallick Road, Kolkata, West Bengal-700032, India.
  • S K Sil Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Abstract

The crude methanol extract of Parkia javanica (crude MEPJ) was screened for immunomodulatory activity on macrophage cells, RAW 264.7, by cell proliferation, migration and qRT PCR based interleukin expression assays. The maximum proliferation and migration of macrophage cells were observed at the dose of 20μg/ml. The expression of proinflammatory cytokines, IL-6, IL-8, IL-12 and TNF-α were increased after treatment with crude MEPJ. This study clearly suggests the immune stimulant property of Parkia javanica on macrophage cells.

Keywords: Parkia javanica, macrophage cells, interleukins, immunomodulatory.

 

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Author Biographies

S Saha, Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

M Dinda, Department of Life Science and Biotechnology, Jadavpur University, 188, Raja S. C. Mallick Road, Kolkata, West Bengal-700032, India.

Department of Life Science and Biotechnology, Jadavpur University, 188, Raja S. C. Mallick Road, Kolkata, West Bengal-700032, India.

P Karmakar, Department of Life Science and Biotechnology, Jadavpur University, 188, Raja S. C. Mallick Road, Kolkata, West Bengal-700032, India.

Department of Life Science and Biotechnology, Jadavpur University, 188, Raja S. C. Mallick Road, Kolkata, West Bengal-700032, India.

S K Sil, Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

Molecular Genetics and Cell Physiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura-799022, India.

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How to Cite
Saha, S., Dinda, M., Karmakar, P., & Sil, S. (2018). IMMUNOMODULATORY EFFECT OF PARKIA JAVANICA EXTRACT ON INTRACELLULAR EXPRESSION OF IL-6, IL-8, IL-12 AND TNF-α. Journal of Drug Delivery and Therapeutics, 8(3), 58-63. https://doi.org/10.22270/jddt.v8i3.1768