IMMUNOMODULATORY EFFECT OF PARKIA JAVANICA EXTRACT ON INTRACELLULAR EXPRESSION OF IL-6, IL-8, IL-12 AND TNF-Î±
The crude methanol extract of Parkia javanica (crude MEPJ) was screened for immunomodulatory activity on macrophage cells, RAW 264.7, by cell proliferation, migration and qRT PCR based interleukin expression assays. The maximum proliferation and migration of macrophage cells were observed at the dose of 20Î¼g/ml. The expression of proinflammatory cytokines, IL-6, IL-8, IL-12 and TNF-Î± were increased after treatment with crude MEPJ. This study clearly suggests the immune stimulant property of Parkia javanica on macrophage cells.
Keywords: Parkia javanica, macrophage cells, interleukins, immunomodulatory.
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